Rsk-4 Antibody - #AF9188

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Product: Rsk-4 Antibody
Catalog: AF9188
Description: Rabbit polyclonal antibody to Rsk-4
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Horse, Rabbit
Mol.Wt.: 84kDa; 84kD(Calculated).
Uniprot: Q9UK32
RRID: AB_2843378

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 100ul $280 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(90%), Horse(80%), Rabbit(80%)
Clonality:
Polyclonal
Specificity:
Rsk-4 Antibody detects endogenous levels of total Rsk-4.
RRID:
AB_2843378
Cite Format: Affinity Biosciences Cat# AF9188, RRID:AB_2843378.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

90 kDa ribosomal protein S6 kinase 6; KS6A6_HUMAN; p90 RSK 6; p90-RSK 6; p90RSK6; pp90RSK4; Ribosomal protein S6 kinase 90kDa polypeptide 6; Ribosomal protein S6 kinase alpha 6; Ribosomal protein S6 kinase alpha-6; Ribosomal S6 kinase 4; rps6ka6; RSK 4; RSK-4; S6K-alpha-6;

Immunogens

Immunogen:
Uniprot:

Q9UK32(KS6A6_HUMAN) >>Visit HPA database.

Gene(ID):
RPS6KA6(27330)
Sequence:
MLPFAPQDEPWDREMEVFSGGGASSGEVNGLKMVDEPMEEGEADSCHDEGVVKEIPITHHVKEGYEKADPAQFELLKVLGQGSFGKVFLVRKKTGPDAGQLYAMKVLKKASLKVRDRVRTKMERDILVEVNHPFIVKLHYAFQTEGKLYLILDFLRGGDVFTRLSKEVLFTEEDVKFYLAELALALDHLHQLGIVYRDLKPENILLDEIGHIKLTDFGLSKESVDQEKKAYSFCGTVEYMAPEVVNRRGHSQSADWWSYGVLMFEMLTGTLPFQGKDRNETMNMILKAKLGMPQFLSAEAQSLLRMLFKRNPANRLGSEGVEEIKRHLFFANIDWDKLYKREVQPPFKPASGKPDDTFCFDPEFTAKTPKDSPGLPASANAHQLFKGFSFVATSIAEEYKITPITSANVLPIVQINGNAAQFGEVYELKEDIGVGSYSVCKRCIHATTNMEFAVKIIDKSKRDPSEEIEILMRYGQHPNIITLKDVFDDGRYVYLVTDLMKGGELLDRILKQKCFSEREASDILYVISKTVDYLHCQGVVHRDLKPSNILYMDESASADSIRICDFGFAKQLRGENGLLLTPCYTANFVAPEVLMQQGYDAACDIWSLGVLFYTMLAGYTPFANGPNDTPEEILLRIGNGKFSLSGGNWDNISDGAKDLLSHMLHMDPHQRYTAEQILKHSWITHRDQLPNDQPKRNDVSHVVKGAMVATYSALTHKTFQPVLEPVAASSLAQRRSMKKRTSTGL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
90
Horse
80
Rabbit
80
Bovine
78
Sheep
78
Dog
73
Chicken
50
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UK32 As Substrate

Site PTM Type Enzyme
S83 Phosphorylation
K86 Acetylation
K86 Ubiquitination
T94 Phosphorylation
K105 Acetylation
K108 Acetylation
Y149 Phosphorylation
S220 Phosphorylation
Y231 Phosphorylation
S232 Phosphorylation
T236 Phosphorylation
Y239 Phosphorylation
K348 Acetylation
K367 Acetylation
S372 Phosphorylation
S378 Phosphorylation
K386 Acetylation
S389 Phosphorylation
Y437 Phosphorylation
K441 Ubiquitination
Y474 Phosphorylation
T482 Phosphorylation
S521 Phosphorylation
Y525 Phosphorylation
S547 Phosphorylation
S555 Phosphorylation
K570 Ubiquitination
T581 Phosphorylation
T710 Phosphorylation
Y711 Phosphorylation
T715 Phosphorylation
T718 Phosphorylation
S730 Phosphorylation
S736 Phosphorylation

PTMs - Q9UK32 As Enzyme

Substrate Site Source
Q15831 (STK11) S428 Uniprot

Research Backgrounds

Function:

Constitutively active serine/threonine-protein kinase that exhibits growth-factor-independent kinase activity and that may participate in p53/TP53-dependent cell growth arrest signaling and play an inhibitory role during embryogenesis.

PTMs:

Phosphorylated at Ser-232, Ser-372, and Ser-389 in serum-starved cells.

Subcellular Location:

Cytoplasm>Cytosol. Nucleus.
Note: Predominantly cytosolic.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Forms a complex with MAPK3/ERK1 but not with MAPK9 or MAPK14 in serum-starved cells.

Family&Domains:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

Restrictive clause

 

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