RAB3IP Antibody - #AF9176

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Product: RAB3IP Antibody
Catalog: AF9176
Description: Rabbit polyclonal antibody to RAB3IP
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 53kDa; 53kD(Calculated).
Uniprot: Q96QF0
RRID: AB_2843366

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(92%), Rabbit(92%), Dog(92%), Chicken(92%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
RAB3IP Antibody detects endogenous levels of total RAB3IP.
RRID:
AB_2843366
Cite Format: Affinity Biosciences Cat# AF9176, RRID:AB_2843366.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Gtpat12; Rab-3A-interacting protein; RAB3A interacting protein; RAB3A interacting protein (rabin3); RAB3A interacting protein; RAB3A-interacting protein; RAB3I_HUMAN; Rab3ip; Rabin 3; Rabin-3; RABIN3; RABIN3, rat, homolog of; Rabin8; SSX2 interacting protein; SSX2 interacting protein; SSX2-interacting protein;

Immunogens

Immunogen:
Uniprot:

Q96QF0(RAB3I_HUMAN) >>Visit HPA database.

Gene(ID):
RAB3IP(117177)
Expression:
Q96QF0 RAB3I_HUMAN:

Expressed in brain, kidney, heart, pancreas and placenta. Not detected in skeletal muscle or liver.

Sequence:
MGLKKMKGLSYDEAFAMANDPLEGFHEVNLASPTSPDLLGVYESGTQEQTTSPSVIYRPHPSALSSVPIQANALDVSELPTQPVYSSPRRLNCAEISSISFHVTDPAPCSTSGVTAGLTKLTTRKDNYNAEREFLQGATITEACDGSDDIFGLSTDSLSRLRSPSVLEVREKGYERLKEELAKAQRELKLKDEECERLSKVRDQLGQELEELTASLFEEAHKMVREANIKQATAEKQLKEAQGKIDVLQAEVAALKTLVLSSSPTSPTQEPLPGGKTPFKKGHTRNKSTSSAMSGSHQDLSVIQPIVKDCKEADLSLYNEFRLWKDEPTMDRTCPFLDKIYQEDIFPCLTFSKSELASAVLEAVENNTLSIEPVGLQPIRFVKASAVECGGPKKCALTGQSKSCKHRIKLGDSSNYYYISPFCRYRITSVCNFFTYIRYIQQGLVKQQDVDQMFWEVMQLRKEMSLAKLGYFKEEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Horse
92
Dog
92
Xenopus
92
Zebrafish
92
Chicken
92
Rabbit
92
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96QF0 As Substrate

Site PTM Type Enzyme
Y85 Phosphorylation
S87 Phosphorylation
T115 Phosphorylation
S154 Phosphorylation
S163 Phosphorylation
S165 Phosphorylation
S262 Phosphorylation
S263 Phosphorylation
T265 Phosphorylation
S266 Phosphorylation
T268 Phosphorylation
T277 Phosphorylation
S288 Phosphorylation Q9Y2H1 (STK38L) , Q15208 (STK38)
T289 Phosphorylation
S296 Phosphorylation
S301 Phosphorylation
K311 Ubiquitination
K325 Ubiquitination
T329 Phosphorylation
K383 Acetylation
K383 Ubiquitination
Y416 Phosphorylation
K462 Methylation
Y471 Phosphorylation

Research Backgrounds

Function:

Guanine nucleotide exchange factor (GEF) which may activate RAB8A and RAB8B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Mediates the release of GDP from RAB8A and RAB8B but not from RAB3A or RAB5. Modulates actin organization and promotes polarized transport of RAB8A-specific vesicles to the cell surface. Together with RAB11A, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis.

Subcellular Location:

Cytoplasm. Nucleus. Cytoplasm>Cytoskeleton. Cell projection>Lamellipodium.
Note: Predominantly cytoplasmic but a small proportion colocalizes with SSX2 in the nucleus. Activation of protein kinase C results in redistribution to the periphery of lamellipodia. In the cytoskeleton, localizes to cortical actin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in brain, kidney, heart, pancreas and placenta. Not detected in skeletal muscle or liver.

Subunit Structure:

Interacts with the N-terminal region of SSX2. Interacts with the GDP-bound forms of RAB8A and RAB8B. The interaction with RAB8A is prevented by phosphorylation of RAB8A at 'Thr-72'. Interacts with the GDP-bound forms of RAB3A and RAB3D. Interacts with DCDC1.

Family&Domains:

Belongs to the SEC2 family.

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