Product: PTP IA-2beta Antibody
Catalog: AF9171
Description: Rabbit polyclonal antibody to PTP IA-2beta
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Dog
Mol.Wt.: 111kDa; 111kD(Calculated).
Uniprot: Q92932
RRID: AB_2843361

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Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(80%), Dog(91%)
Clonality:
Polyclonal
Specificity:
PTP IA-2beta Antibody detects endogenous levels of total PTP IA-2beta.
RRID:
AB_2843361
Cite Format: Affinity Biosciences Cat# AF9171, RRID:AB_2843361.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

IA 2beta; IA2beta; IAR; IAR/receptor like protein tyrosine phosphatase; ICAAR; Islet cell autoantigen related protein; Islet cell autoantigen-related protein; Phogrin; Protein tyrosine phosphatase receptor pi; Protein tyrosine phosphatase, receptor type, N polypeptide 2; PTPR2_HUMAN; Ptprn2; PTPRP; R PTP N2; R-PTP-N2; Receptor-type tyrosine-protein phosphatase N2; Tyrosine phosphatase IA 2 beta;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q92932 PTPR2_HUMAN:

Highest levels in brain and pancreas (PubMed:8954911, PubMed:8798755). Lower levels in trachea, prostate, stomach and spinal chord (PubMed:8798755).

Sequence:
MGPPLPLLLLLLLLLPPRVLPAAPSSVPRGRQLPGRLGCLLEEGLCGASEACVNDGVFGRCQKVPAMDFYRYEVSPVALQRLRVALQKLSGTGFTWQDDYTQYVMDQELADLPKTYLRRPEASSPARPSKHSVGSERRYSREGGAALANALRRHLPFLEALSQAPASDVLARTHTAQDRPPAEGDDRFSESILTYVAHTSALTYPPGSRTQLREDLLPRTLGQLQPDELSPKVDSGVDRHHLMAALSAYAAQRPPAPPGEGSLEPQYLLRAPSRMPRPLLAPAAPQKWPSPLGDSEDPSSTGDGARIHTLLKDLQRQPAEVRGLSGLELDGMAELMAGLMQGVDHGVARGSPGRAALGESGEQADGPKATLRGDSFPDDGVQDDDDRLYQEVHRLSATLGGLLQDHGSRLLPGALPFARPLDMERKKSEHPESSLSSEEETAGVENVKSQTYSKDLLGQQPHSEPGAAAFGELQNQMPGPSKEEQSLPAGAQEALSDGLQLEVQPSEEEARGYIVTDRDPLRPEEGRRLVEDVARLLQVPSSAFADVEVLGPAVTFKVSANVQNVTTEDVEKATVDNKDKLEETSGLKILQTGVGSKSKLKFLPPQAEQEDSTKFIALTLVSLACILGVLLASGLIYCLRHSSQHRLKEKLSGLGGDPGADATAAYQELCRQRMATRPPDRPEGPHTSRISSVSSQFSDGPIPSPSARSSASSWSEEPVQSNMDISTGHMILSYMEDHLKNKNRLEKEWEALCAYQAEPNSSFVAQREENVPKNRSLAVLTYDHSRVLLKAENSHSHSDYINASPIMDHDPRNPAYIATQGPLPATVADFWQMVWESGCVVIVMLTPLAENGVRQCYHYWPDEGSNLYHIYEVNLVSEHIWCEDFLVRSFYLKNLQTNETRTVTQFHFLSWYDRGVPSSSRSLLDFRRKVNKCYRGRSCPIIVHCSDGAGRSGTYVLIDMVLNKMAKGAKEIDIAATLEHLRDQRPGMVQTKEQFEFALTAVAEEVNAILKALPQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
91
Pig
80
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92932 As Substrate

Site PTM Type Enzyme
S25 Phosphorylation
S26 Phosphorylation
S124 Phosphorylation
S129 Phosphorylation
Y139 Phosphorylation
T199 Phosphorylation
T220 O-Glycosylation
T220 Phosphorylation
S230 O-Glycosylation
S230 Phosphorylation
S247 Phosphorylation
Y249 Phosphorylation
S360 Phosphorylation
T398 O-Glycosylation
S433 Phosphorylation
S434 Phosphorylation
S436 Phosphorylation
S437 Phosphorylation
T619 Phosphorylation
S622 Phosphorylation
S643 Phosphorylation
Y666 Phosphorylation
S695 Phosphorylation
S704 Phosphorylation
S776 Phosphorylation
K970 Acetylation
T977 Phosphorylation

Research Backgrounds

Function:

Plays a role in vesicle-mediated secretory processes. Required for normal accumulation of secretory vesicles in hippocampus, pituitary and pancreatic islets. Required for the accumulation of normal levels of insulin-containing vesicles and preventing their degradation. Plays a role in insulin secretion in response to glucose stimuli. Required for normal accumulation of the neurotransmitters norepinephrine, dopamine and serotonin in the brain. In females, but not in males, required for normal accumulation and secretion of pituitary hormones, such as luteinizing hormone (LH) and follicle-stimulating hormone (FSH) (By similarity). Required to maintain normal levels of renin expression and renin release (By similarity). May regulate catalytic active protein-tyrosine phosphatases such as PTPRA through dimerization (By similarity). Has phosphatidylinositol phosphatase activity; the PIPase activity is involved in its ability to regulate insulin secretion. Can dephosphorylate phosphatidylinositol 4,5-biphosphate (PI(4,5)P2), phosphatidylinositol 5-phosphate and phosphatidylinositol 3-phosphate (By similarity). Regulates PI(4,5)P2 level in the plasma membrane and localization of cofilin at the plasma membrane and thus is indirectly involved in regulation of actin dynamics related to cell migration and metastasis; upon hydrolyzation of PI(4,5)P2 cofilin is released from the plasma membrane and acts in the cytoplasm in severing F-actin filaments.

PTMs:

Subject to proteolytic cleavage at multiple sites.

Subcellular Location:

Cytoplasmic vesicle>Secretory vesicle membrane>Single-pass type I membrane protein. Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle membrane>Single-pass type I membrane protein.
Note: Predominantly found on dense-core secretory granules. Sorting to secretory granules in part is dependent of the N-terminal propeptide domain of the precursor and its interaction with CPE (By similarity). Transiently found at the cell membrane, when secretory vesicles fuse with the cell membrane to release their cargo. Is then endocytosed and recycled to secretory vesicles involving clathrin-dependent AP2-mediated endocytosis. Recycled via STX6- but not TTTGN1/TGN38-containing compartments (By similarity).

Cytoplasmic vesicle>Secretory vesicle membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highest levels in brain and pancreas. Lower levels in trachea, prostate, stomach and spinal chord.

Subunit Structure:

Self-associates. Interacts (via cytoplasmic domain) with PTPRN (via cytoplasmic domain). Interacts (precursor form) with CPE. Interacts with HAP1. Interacts with AP2A1 or AP2A2 and AP1G1; indicative for an association with adaptor protein complex 2 (AP-2) and adaptor protein complex 1 (AP-1) (By similarity). Interacts with AP2M1; indicative for an association with adaptor protein complex 2 (AP-2). Interacts with MYO5A (By similarity).

Family&Domains:

The cytoplasmic domain appears to contain the autoantigenic epitopes.

The leucine-based sorting signal is proposed to function in trafficking at the plasma membrane.

The tyrosine-based internalization signal is proposed to function at the level of clathrin-mediated endocytosis and recycling.

Belongs to the protein-tyrosine phosphatase family. Receptor class 8 subfamily.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Type I diabetes mellitus.

References

1). HOXD13 promotes the malignant progression of colon cancer by upregulating PTPRN2. Cancer Medicine, 2021 (PubMed: 34272834) [IF=4.0]

Application: WB    Species: Human    Sample: LoVo and CW- 2 cells

FIGURE 4 PTPRN2 promotes colon cancer cell progression. (A) Protein levels of PTPRN2 in LoVo and CW- 2 cells after treatment. (B) Cell invasive ability determined by Transwell assay analysis. (C) Cell migration detected by wound healing. (D) Proliferation ability detected by clone formation experiment

Application: IHC    Species: Human    Sample: LoVo and CW- 2 cells

FIGURE 4 PTPRN2 promotes colon cancer cell progression. (A) Protein levels of PTPRN2 in LoVo and CW- 2 cells after treatment. (B) Cell invasive ability determined by Transwell assay analysis. (C) Cell migration detected by wound healing. (D) Proliferation ability detected by clone formation experiment

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