PRPF31 Antibody - #AF9169

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Product: PRPF31 Antibody
Catalog: AF9169
Description: Rabbit polyclonal antibody to PRPF31
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Rabbit, Xenopus
Mol.Wt.: 55kDa; 55kD(Calculated).
Uniprot: Q8WWY3
RRID: AB_2843359

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
PRPF31 Antibody detects endogenous levels of total PRPF31.
RRID:
AB_2843359
Cite Format: Affinity Biosciences Cat# AF9169, RRID:AB_2843359.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp566J153; hPrp 31; hPrp31; NY BR 99; Pre mRNA processing factor 31; Pre mRNA processing factor 31 homolog (yeast); Pre mRNA processing factor 31 homolog; Pre-mRNA-processing factor 31; Precursor mRNA-processing factor 31, S. cerevisiae, homolog of; Protein 61K; PRP 31; PRP31; PRP31 pre mRNA processing factor 31 homolog (yeast); PRP31 pre mRNA processing factor 31 homolog; PRP31_HUMAN; PRPF 31; prpf31; RP 11; RP11; Serologically defined breast cancer antigen NY BR 99; Serologically defined breast cancer antigen NY-BR-99; SNRNP61; U4/U6 small nuclear ribonucleoprotein Prp31; U4/U6 snRNP 61 kDa protein;

Immunogens

Immunogen:
Uniprot:

Q8WWY3(PRP31_HUMAN) >>Visit HPA database.

Gene(ID):
PRPF31(26121)
Expression:
Q8WWY3 PRP31_HUMAN:

Ubiquitously expressed.

Sequence:
MSLADELLADLEEAAEEEEGGSYGEEEEEPAIEDVQEETQLDLSGDSVKTIAKLWDSKMFAEIMMKIEEYISKQAKASEVMGPVEAAPEYRVIVDANNLTVEIENELNIIHKFIRDKYSKRFPELESLVPNALDYIRTVKELGNSLDKCKNNENLQQILTNATIMVVSVTASTTQGQQLSEEELERLEEACDMALELNASKHRIYEYVESRMSFIAPNLSIIIGASTAAKIMGVAGGLTNLSKMPACNIMLLGAQRKTLSGFSSTSVLPHTGYIYHSDIVQSLPPDLRRKAARLVAAKCTLAARVDSFHESTEGKVGYELKDEIERKFDKWQEPPPVKQVKPLPAPLDGQRKKRGGRRYRKMKERLGLTEIRKQANRMSFGEIEEDAYQEDLGFSLGHLGKSGSGRVRQTQVNEATKARISKTLQRTLQKQSVVYGGKSTIRDRSSGTASSVAFTPLQGLEIVNPQAAEKKVAEANQKYFSSMAEFLKVKGEKSGLMST

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Xenopus
100
Zebrafish
100
Rabbit
100
Sheep
36
Pig
0
Dog
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WWY3 As Substrate

Site PTM Type Enzyme
K53 Ubiquitination
Y70 Phosphorylation
K73 Ubiquitination
K76 Ubiquitination
Y90 Phosphorylation
K140 Ubiquitination
K148 Ubiquitination
Y205 Phosphorylation
Y207 Phosphorylation
S210 Phosphorylation
Y273 Phosphorylation
K298 Sumoylation
K298 Ubiquitination
S307 Phosphorylation
K321 Sumoylation
K321 Ubiquitination
K327 Sumoylation
K330 Ubiquitination
K338 Sumoylation
K338 Ubiquitination
S379 Phosphorylation
Y388 Phosphorylation
S395 Phosphorylation
K401 Ubiquitination
T410 Phosphorylation
T416 Phosphorylation
K417 Sumoylation
K417 Ubiquitination
K422 Sumoylation
K430 Sumoylation
K430 Ubiquitination
S432 Phosphorylation
Y435 Phosphorylation
K438 Acetylation
K438 Methylation
K438 Ubiquitination
S439 Phosphorylation
T440 Phosphorylation
S445 Phosphorylation P24941 (CDK2)
S446 Phosphorylation P24941 (CDK2)
T448 Phosphorylation
S450 Phosphorylation P24941 (CDK2)
S451 Phosphorylation P24941 (CDK2)
T455 Phosphorylation P24941 (CDK2)
K470 Acetylation
K478 Sumoylation
K478 Ubiquitination
Y479 Phosphorylation
S481 Phosphorylation
S482 Phosphorylation
K493 Ubiquitination
S494 Phosphorylation
S498 Phosphorylation
T499 Phosphorylation

Research Backgrounds

Function:

Involved in pre-mRNA splicing as component of the spliceosome. Required for the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.

Subcellular Location:

Nucleus. Nucleus speckle. Nucleus>Cajal body.
Note: Predominantly found in speckles and in Cajal bodies.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Identified in the spliceosome B complex. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39. Interacts with a complex formed by SNU13 and U4 snRNA, but not with SNU13 or U4 snRNA alone. The complex formed by SNU13 and PRPF31 binds also U4atac snRNA, a characteristic component of specific, less abundant spliceosomal complexes. Interacts with PRPF6/U5 snRNP-associated 102 kDa protein. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts (via its NLS) with CTNNBL1.

Family&Domains:

Interacts with the snRNP via the Nop domain.

The coiled coil domain is formed by two non-contiguous helices.

Belongs to the PRP31 family.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

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