PNPase Antibody - #AF9164

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Product: PNPase Antibody
Catalog: AF9164
Description: Rabbit polyclonal antibody to PNPase
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Rat, Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 85kDa; 86kD(Calculated).
Uniprot: Q8TCS8
RRID: AB_2843354

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Rat(%), Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(90%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
PNPase Antibody detects endogenous levels of total PNPase.
RRID:
AB_2843354
Cite Format: Affinity Biosciences Cat# AF9164, RRID:AB_2843354.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

3' 5' RNA exonuclease; COXPD13; DFNB70; old-35; OLD35; PNPASE; PNPase 1; Polynucleotide phosphorylase like protein; Polyribonucleotide nucleotidyltransferase 1, mitochondrial;

Immunogens

Immunogen:
Uniprot:

Q8TCS8(PNPT1_HUMAN) >>Visit HPA database.

Gene(ID):
PNPT1(87178)
Sequence:
MAACRYCCSCLRLRPLSDGPFLLPRRDRALTQLQVRALWSSAGSRAVAVDLGNRKLEISSGKLARFADGSAVVQSGDTAVMVTAVSKTKPSPSQFMPLVVDYRQKAAAAGRIPTNYLRREIGTSDKEILTSRIIDRSIRPLFPAGYFYDTQVLCNLLAVDGVNEPDVLAINGASVALSLSDIPWNGPVGAVRIGIIDGEYVVNPTRKEMSSSTLNLVVAGAPKSQIVMLEASAENILQQDFCHAIKVGVKYTQQIIQGIQQLVKETGVTKRTPQKLFTPSPEIVKYTHKLAMERLYAVFTDYEHDKVSRDEAVNKIRLDTEEQLKEKFPEADPYEIIESFNVVAKEVFRSIVLNEYKRCDGRDLTSLRNVSCEVDMFKTLHGSALFQRGQTQVLCTVTFDSLESGIKSDQVITAINGIKDKNFMLHYEFPPYATNEIGKVTGLNRRELGHGALAEKALYPVIPRDFPFTIRVTSEVLESNGSSSMASACGGSLALMDSGVPISSAVAGVAIGLVTKTDPEKGEIEDYRLLTDILGIEDYNGDMDFKIAGTNKGITALQADIKLPGIPIKIVMEAIQQASVAKKEILQIMNKTISKPRASRKENGPVVETVQVPLSKRAKFVGPGGYNLKKLQAETGVTISQVDEETFSVFAPTPSAMHEARDFITEICKDDQEQQLEFGAVYTATITEIRDTGVMVKLYPNMTAVLLHNTQLDQRKIKHPTALGLEVGQEIQVKYFGRDPADGRMRLSRKVLQSPATTVVRTLNDRSSIVMGEPISQSSSNSQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
90
Chicken
90
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8TCS8 As Substrate

Site PTM Type Enzyme
S59 Phosphorylation
S60 Phosphorylation
K62 Ubiquitination
S91 Phosphorylation
Y102 Phosphorylation
K126 Acetylation
Y200 Phosphorylation
K250 Acetylation
K264 Acetylation
T272 Phosphorylation
K275 Acetylation
K275 Ubiquitination
T278 Phosphorylation
S280 Phosphorylation
K285 Acetylation
K285 Ubiquitination
K289 Acetylation
Y296 Phosphorylation
T300 Phosphorylation
Y302 Phosphorylation
K306 Acetylation
K306 Ubiquitination
K315 Ubiquitination
K327 Ubiquitination
Y334 Phosphorylation
S339 Phosphorylation
S350 Phosphorylation
Y356 Phosphorylation
K357 Ubiquitination
K378 Ubiquitination
K419 Ubiquitination
K439 Acetylation
K456 Ubiquitination
Y459 Phosphorylation
T473 Phosphorylation
S474 Phosphorylation
K521 Acetylation
K521 Ubiquitination
K569 Methylation
K591 Acetylation
K591 Ubiquitination
T592 Phosphorylation
K601 Ubiquitination
Y626 Phosphorylation
K629 Ubiquitination
T665 Phosphorylation
T683 Phosphorylation
T687 Phosphorylation
T721 Phosphorylation
S754 Phosphorylation
T758 Phosphorylation
S776 Phosphorylation P78527 (PRKDC)
S782 Phosphorylation

Research Backgrounds

Function:

RNA-binding protein implicated in numerous RNA metabolic processes. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonulease activity. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules. Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration; regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity; degrades c-myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells; specifically and selectively degrades miR-221, preferentially. Plays also a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA.

Subcellular Location:

Cytoplasm. Mitochondrion matrix. Mitochondrion intermembrane space>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homotrimer; in free form. Homooligomer. Component of the mitochondrial degradosome (mtEXO) complex which is a heteropentamer containing 2 copies of SUPV3L1 and 3 copies of PNPT1. As part of the mitochondrial degradosome complex, interacts with GRSF1 in an RNA-dependent manner; the interaction enhances the activity of the complex. Interacts with TCL1A; the interaction has no effect on PNPT1 exonuclease activity.

Family&Domains:

Belongs to the polyribonucleotide nucleotidyltransferase family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

Restrictive clause

 

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