Product: PIG3 Antibody
Catalog: AF9159
Description: Rabbit polyclonal antibody to PIG3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Bovine, Horse, Sheep
Mol.Wt.: 36kDa; 36kD(Calculated).
Uniprot: Q53FA7
RRID: AB_2843349

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Bovine(83%), Horse(92%), Sheep(83%)
Clonality:
Polyclonal
Specificity:
PIG3 Antibody detects endogenous levels of total PIG3.
RRID:
AB_2843349
Cite Format: Affinity Biosciences Cat# AF9159, RRID:AB_2843349.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

p53 induced gene 3 protein; p53-induced gene 3 protein; Putative quinone oxidoreductase; QORX_HUMAN; quinone oxidoreductase homolog; Quinone oxidoreductase PIG3; TP53I3; Tumor protein p53 inducible protein 3; Tumor protein p53-inducible protein 3;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MLAVHFDKPGGPENLYVKEVAKPSPGEGEVLLKVAASALNRADLMQRQGQYDPPPGASNILGLEASGHVAELGPGCQGHWKIGDTAMALLPGGGQAQYVTVPEGLLMPIPEGLTLTQAAAIPEAWLTAFQLLHLVGNVQAGDYVLIHAGLSGVGTAAIQLTRMAGAIPLVTAGSQKKLQMAEKLGAAAGFNYKKEDFSEATLKFTKGAGVNLILDCIGGSYWEKNVNCLALDGRWVLYGLMGGGDINGPLFSKLLFKRGSLITSLLRSRDNKYKQMLVNAFTEQILPHFSTEGPQRLLPVLDRIYPVTEIQEAHKYMEANKNIGKIVLELPQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
92
Bovine
83
Sheep
83
Pig
75
Dog
75
Rabbit
75
Xenopus
67
Chicken
58
Zebrafish
50
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q53FA7 As Substrate

Site PTM Type Enzyme
T85 Phosphorylation
T100 Phosphorylation
K194 Ubiquitination
K203 Ubiquitination
S260 Phosphorylation
T263 Phosphorylation
S264 Phosphorylation
K321 Acetylation

Research Backgrounds

Function:

May be involved in the generation of reactive oxygen species (ROS). Has low NADPH-dependent beta-naphthoquinone reductase activity, with a preference for 1,2-beta-naphthoquinone over 1,4-beta-naphthoquinone. Has low NADPH-dependent diamine reductase activity (in vitro).

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.

Research Fields

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.