PI 3-kinase p101 Antibody | Affinity Biosciences

1/1

Western blot analysis of PI 3-kinase p101 using HeLa whole cell lysates.

Product:	PI 3-kinase p101 Antibody
Catalog:	AF9158
Description:	Rabbit polyclonal antibody to PI 3-kinase p101
Application:	WB
Reactivity:	Human
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.:	100kDa; 97kD(Calculated).
Uniprot:	Q8WYR1
RRID:	AB_2843348

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(88%), Sheep(100%), Dog(100%), Chicken(88%)

Clonality:

Polyclonal

Specificity:

PI 3-kinase p101 Antibody detects endogenous levels of total PI 3-kinase p101.

RRID:

AB_2843348
Cite Format: Affinity Biosciences Cat# AF9158, RRID:AB_2843348.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

5-bisphosphate 3-kinase regulatory subunit; F730038I15Rik; FOAP 2; FOAP2; OTTHUMP00000135339; p101; P101 PI3K; p101-PI3K; Phosphatidylinositol 4; Phosphatidylinositol 4,5 bisphosphate 3 kinase regulatory subunit; Phosphoinositide 3 kinase, regulatory subunit 5; Phosphoinositide 3 kinase, regulatory subunit 5, p101; Phosphoinositide 3 kinase, regulatory subunit, polypeptide p101; Phosphoinositide 3-kinase regulatory subunit 5; PI3 kinase p101 subunit; PI3 kinase regulatory subunit 5; PI3-kinase p101 subunit; PI3-kinase regulatory subunit 5; PI3R5_HUMAN; PIK3 R5; PIK3R5; Protein FOAP 2; Protein FOAP-2; PtdIns 3 kinase p101; PtdIns 3 kinase regulatory subunit; PtdIns-3-kinase p101; PtdIns-3-kinase regulatory subunit;

Immunogens

Immunogen:

Uniprot:

Q8WYR1(PI3R5_HUMAN) >>Visit HPA database.

Gene(ID):

PIK3R5(23533)

Expression:

Q8WYR1 PI3R5_HUMAN:

Ubiquitously expressed with high expression in fetal brain compared to adult brain. Abundant expression is observed in cerebellum, cerebral cortex, cerebral meninges, and vermis cerebelli.

Sequence:

Q8WYR1 PI3R5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MQPGATTCTEDRIQHALERCLHGLSLSRRSTSWSAGLCLNCWSLQELVSRDPGHFLILLEQILQKTREVQEKGTYDLLTPLALLFYSTVLCTPHFPPDSDLLLKAASTYHRFLTWPVPYCSICQELLTFIDAELKAPGISYQRLVRAEQGLPIRSHRSSTVTVLLLNPVEVQAEFLAVANKLSTPGHSPHSAYTTLLLHAFQATFGAHCDVPGLHCRLQAKTLAELEDIFTETAEAQELASGIGDAAEARRWLRTKLQAVGEKAGFPGVLDTAKPGKLHTIPIPVARCYTYSWSQDSFDILQEILLKEQELLQPGILGDDEEEEEEEEEVEEDLETDGHCAERDSLLSTSSLASHDSTLSLASSQASGPALSRHLLTSFVSGLSDGMDSGYVEDSEESSSEWPWRRGSQERRGHRRPGQKFIRIYKLFKSTSQLVLRRDSRSLEGSSDTALPLRRAGSLCSPLDEPVSPPSRAQRSRSLPQPKLGTQLPSWLLAPASRPQRRRPFLSGDEDPKASTLRVVVFGSDRISGKVARAYSNLRRLENNRPLLTRFFKLQFFYVPVKRSHGTSPGACPPPRSQTPSPPTDSPRHASPGELGTTPWEESTNDISHYLGMLDPWYERNVLGLMHLPPEVLCQQSLKAEAQALEGSPTQLPILADMLLYYCRFAARPVLLQVYQTELTFITGEKTTEIFIHSLELGHSAATRAIKASGPGSKRLGIDGDREAVPLTLQIIYSKGAISGRSRWSNLEKVCTSVNLNKACRKQEELDSSMEALTLNLTEVVKRQNSKSKKGFNQISTSQIKVDKVQIIGSNSCPFAVCLDQDERKILQSVVRCEVSPCYKPEKSDLSSPPQTPPDLPAQAAPDLCSLLCLPIMTFSGALP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Zebrafish

100

Horse

Chicken

Rabbit

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WYR1 As Substrate

Q8WYR1

Site	PTM Type	Source
T114	Phosphorylation	Uniprot
S121	Phosphorylation	Uniprot
K256	Ubiquitination	Uniprot
K263	Ubiquitination	Uniprot
K429	Ubiquitination	Uniprot
S430	Phosphorylation	Uniprot
S432	Phosphorylation	Uniprot
S440	Phosphorylation	Uniprot
S447	Phosphorylation	Uniprot
S458	Phosphorylation	Uniprot
S468	Phosphorylation	Uniprot
S507	Phosphorylation	Uniprot
K562	Ubiquitination	Uniprot
K758	Ubiquitination	Uniprot
K782	Acetylation	Uniprot
K825	Ubiquitination	Uniprot

Research Backgrounds

Q8WYR1_PI3R5_HUMAN

Function:

Regulatory subunit of the PI3K gamma complex. Required for recruitment of the catalytic subunit to the plasma membrane via interaction with beta-gamma G protein dimers. Required for G protein-mediated activation of PIK3CG (By similarity).

Subcellular Location:

Nucleus. Cytoplasm. Cell membrane>Peripheral membrane protein.
Note: Predominantly localized in the nucleus in absence of PIK3CG/p120. Colocalizes with PIK3CG/p120 in the cytoplasm. Translocated to the plasma membrane in a beta-gamma G protein-dependent manner.

Tissue Specificity:

Ubiquitously expressed with high expression in fetal brain compared to adult brain. Abundant expression is observed in cerebellum, cerebral cortex, cerebral meninges, and vermis cerebelli.

Subunit Structure:

Heterodimer of a catalytic subunit (PIK3CG/p120) and a regulatory (PIK3R5a/p101) subunit. Interacts with beta-gamma G protein dimers.

Family&Domains:

The heterodimerization region allows the binding to the catalytic subunit.

Research Fields

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)

· Organismal Systems > Immune system > Platelet activation. (View pathway)

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

PI 3-kinase p101 Antibody - #AF9158