PHKB Antibody - #AF9156
| Product: | PHKB Antibody |
| Catalog: | AF9156 |
| Description: | Rabbit polyclonal antibody to PHKB |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 124kDa; 125kD(Calculated). |
| Uniprot: | Q93100 |
| RRID: | AB_2843346 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF9156, RRID:AB_2843346.
Fold/Unfold
DKFZp781E15103; FLJ41698; KPBB_HUMAN; PHKB; Phosphorylase b kinase regulatory subunit beta; Phosphorylase kinase B; Phosphorylase kinase beta polypeptide; Phosphorylase kinase beta subunit; Phosphorylase kinase subunit beta;
Immunogens
- Q93100 KPBB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGAAGLTAEVSWKVLERRARTKRSGSVYEPLKSINLPRPDNETLWDKLDHYYRIVKSTLLLYQSPTTGLFPTKTCGGDQKAKIQDSLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPTTCLHSVFNVHTGDELLSYEEYGHLQINAVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCISYPAFALDDEVLFSQTLDKVVRKLKGKYGFKRFLRDGYRTSLEDPNRCYYKPAEIKLFDGIECEFPIFFLYMMIDGVFRGNPKQVQEYQDLLTPVLHHTTEGYPVVPKYYYVPADFVEYEKNNPGSQKRFPSNCGRDGKLFLWGQALYIIAKLLADELISPKDIDPVQRYVPLKDQRNVSMRFSNQGPLENDLVVHVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYLQLGINEKLGLSGRPDRPIGCLGTSKIYRILGKTVVCYPIIFDLSDFYMSQDVFLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDMLAALKKGIIGGVKVHVDRLQTLISGAVVEQLDFLRISDTEELPEFKSFEELEPPKHSKVKRQSSTPSAPELGQQPDVNISEWKDKPTHEILQKLNDCSCLASQAILLGILLKREGPNFITKEGTVSDHIERVYRRAGSQKLWLAVRYGAAFTQKFSSSIAPHITTFLVHGKQVTLGAFGHEEEVISNPLSPRVIQNIIYYKCNTHDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGDKPALDLYQLSPSEVKQLLLDILQPQQNGRCWLNRRQIDGSLNRTPTGFYDRVWQILERTPNGIIVAGKHLPQQPTLSDMTMYEMNFSLLVEDTLGNIDQPQYRQIVVELLMVVSIVLERNPELEFQDKVDLDRLVKEAFNEFQKDQSRLKEIEKQDDMTSFYNTPPLGKRGTCSYLTKAVMNLLLEGEVKPNNDDPCLIS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q93100 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S27 | Phosphorylation | Uniprot | |
| Y29 | Phosphorylation | Uniprot | |
| K33 | Ubiquitination | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| T316 | Phosphorylation | Uniprot | |
| Y338 | Phosphorylation | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| Y388 | Phosphorylation | Uniprot | |
| T393 | Phosphorylation | Uniprot | |
| K408 | Ubiquitination | Uniprot | |
| K421 | Ubiquitination | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| K462 | Ubiquitination | Uniprot | |
| K474 | Ubiquitination | Uniprot | |
| S684 | Phosphorylation | Uniprot | |
| S694 | Phosphorylation | Uniprot | |
| S700 | Phosphorylation | Uniprot | |
| S701 | Phosphorylation | Uniprot | |
| T702 | Phosphorylation | Uniprot | |
| S704 | Phosphorylation | Uniprot | |
| K758 | Ubiquitination | Uniprot | |
| R944 | Methylation | Uniprot | |
| K1037 | Ubiquitination | Uniprot | |
| K1043 | Acetylation | Uniprot | |
| K1047 | Ubiquitination | Uniprot |
Research Backgrounds
Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation.
Ser-701 is probably phosphorylated by PKA.
Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated.
Cell membrane>Lipid-anchor>Cytoplasmic side.
Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.
Belongs to the phosphorylase b kinase regulatory chain family.
Research Fields
· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Glucagon signaling pathway.
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