PHKA1/2 Antibody - #AF9155

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Product: PHKA1/2 Antibody
Catalog: AF9155
Description: Rabbit polyclonal antibody to PHKA1/2
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 137kDa; 137kD,138kD(Calculated).
Uniprot: P46020 | P46019
RRID: AB_2843345

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
PHKA1/2 Antibody detects endogenous levels of total PHKA1/2.
RRID:
AB_2843345
Cite Format: Affinity Biosciences Cat# AF9155, RRID:AB_2843345.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5330411D17; 9830108K24Rik; kinase PHKA1; KPB1; KPB1_HUMAN; MGC132604; Pcyt1b; PHKA; PHKA1; Phosphorylase b kinase regulatory subunit alpha; Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform; Phosphorylase kinase alpha M subunit; phosphorylase kinase, alpha 1 (muscle); RP23 210E20.1; skeletal muscle isoform; dJ499B10.2 (phosphorylase kinase, alpha 2 (liver) (PYK)); GSD9A; PHK; PHKLA; Phosphorylase b kinase alpha regulatory chain, liver isoform; phosphorylase b kinase regulatory subunit alpha, liver isoform; Phosphorylase kinase , liver, alpha-2 subunit; Phosphorylase kinase alpha 2; phosphorylase kinase alpha L subunit; phosphorylase kinase alpha subunit; phosphorylase kinase deficiency, liver (glycogen storage disease type VIII; phosphorylase kinase deficiency, liver (glycogen storage disease); phosphorylase kinase, alpha 2 (liver); phosphorylase kinase, alpha 2 (liver), glycogen storage disease IX; PYK; PYKL; XLG; XLG2;

Immunogens

Immunogen:
Uniprot:

P46020(KPB1_HUMAN) >>Visit HPA database.

P46019(KPB2_HUMAN) >>Visit HPA database.

Gene(ID):
PHKA1(5255) | PHKA2(5256)
Expression:
P46020 KPB1_HUMAN:

Muscle specific. Isoform 1 is predominant in vastus lateralis muscle. Isoform 2 predominates slightly in heart, and it predominates clearly in the other tissues tested.

P46019 KPB2_HUMAN:

Predominantly expressed in liver and other non-muscle tissues.

Sequence:
MRSRSNSGVRLDGYARLVQQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSLLPRASTSKEVDASLLSVVSFPAFAVEDSQLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNAEQVQEYKEALEAVLIKGKNGVPLLPELYSVPPDRVDEEYQNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKTILKDKGIYVETIAEVYPIRVQPARILSHIYSSLGCNNRMKLSGRPYRHMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNKMIVEMLRTDLSYLCSRWRMTGQPTITFPISHSMLDEDGTSLNSSILAALRKMQDGYFGGARVQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDDNYDYLESGNWMNDYDSTSHARCGDEVARYLDHLLAHTAPHPKLAPTSQKGGLDRFQAAVQTTCDLMSLVTKAKELHVQNVHMYLPTKLFQASRPSFNLLDSPHPRQENQVPSVRVEIHLPRDQSGEVDFKALVLQLKETSSLQEQADILYMLYTMKGPDWNTELYNERSATVRELLTELYGKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSHQKHLTVGLPPEPREKTISAPLPYEALTQLIDEASEGDMSISILTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATEGLMNLSPSAMKNLLHHILSGKEFGVERSVRPTDSNVSPAISIHEIGAVGATKTERTGIMQLKSEIKQVEFRRLSISAESQSPGTSMTPSSGSFPSAYDQQSSKDSRQGQWQRRRRLDGALNRVPVGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMLADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDTMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSICAMQ

MRSRSNSGVRLDGYARLVQQTILCYQNPVTGLLSASHEQKDAWVRDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVAKVEKFKHTQSTKDSLHAKYNTATCGTVVGDDQWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGMAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPNRLHYDPAELKLFENIECEWPVFWTYFIIDGVFSGDAVQVQEYREALEGILIRGKNGIRLVPELYAVPPNKVDEEYKNPHTVDRVPMGKVPHLWGQSLYILSSLLAEGFLAAGEIDPLNRRFSTSVKPDVVVQVTVLAENNHIKDLLRKHGVNVQSIADIHPIQVQPGRILSHIYAKLGRNKNMNLSGRPYRHIGVLGTSKLYVIRNQIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPISRTMLTNDGSDIHSAVLSTIRKLEDGYFGGARVKLGNLSEFLTTSFYTYLTFLDPDCDEKLFDNASEGTFSPDSDSDLVGYLEDTCNQESQDELDHYINHLLQSTSLRSYLPPLCKNTEDRHVFSAIHSTRDILSVMAKAKGLEVPFVPMTLPTKVLSAHRKSLNLVDSPQPLLEKVPESDFQWPRDDHGDVDCEKLVEQLKDCSNLQDQADILYILYVIKGPSWDTNLSGQHGVTVQNLLGELYGKAGLNQEWGLIRYISGLLRKKVEVLAEACTDLLSHQKQLTVGLPPEPREKIISAPLPPEELTKLIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFVEMLRLRIGLIIQVMATELARSLNCSGEEASESLMNLSPFDMKNLLHHILSGKEFGVERSVRPIHSSTSSPTISIHEVGHTGVTKTERSGINRLRSEMKQMTRRFSADEQFFSVGQAASSSAHSSKSARSSTPSSPTGTSSSDSGGHHIGWGERQGQWLRRRRLDGAINRVPVGFYQRVWKILQKCHGLSIDGYVLPSSTTREMTPHEIKFAVHVESVLNRVPQPEYRQLLVEAIMVLTLLSDTEMTSIGGIIHVDQIVQMASQLFLQDQVSIGAMDTLEKDQATGICHFFYDSAPSGAYGTMTYLTRAVASYLQELLPNSGCQMQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P46020/P46019 As Substrate

Site PTM Type Enzyme
S5 Phosphorylation
S7 Phosphorylation
K286 Ubiquitination
K379 Ubiquitination
K457 Ubiquitination
K485 Ubiquitination
S508 Phosphorylation
Y613 Phosphorylation
Y615 Phosphorylation
T617 Phosphorylation
K682 Ubiquitination
S691 Phosphorylation
S695 Phosphorylation
K707 Ubiquitination
T717 Phosphorylation
S724 Phosphorylation
K728 Ubiquitination
S729 Phosphorylation
S735 Phosphorylation
K742 Ubiquitination
K762 Ubiquitination
S771 Phosphorylation
S796 Phosphorylation
T802 Phosphorylation
Y811 Phosphorylation
K962 Ubiquitination
S969 Phosphorylation
S976 Phosphorylation
T977 Phosphorylation
S978 Phosphorylation
S979 Phosphorylation
T981 Phosphorylation
S983 Phosphorylation
T1011 Phosphorylation
S1015 Phosphorylation
S1022 Phosphorylation
T1041 Phosphorylation
S1043 Phosphorylation
S1044 Phosphorylation
T1046 Phosphorylation
S1051 Phosphorylation
Site PTM Type Enzyme
S5 Phosphorylation
S7 Phosphorylation
K73 Ubiquitination
K109 Ubiquitination
K115 Ubiquitination
Y312 Phosphorylation
K361 Ubiquitination
S405 Phosphorylation
Y407 Phosphorylation
S431 Phosphorylation
T432 Phosphorylation
K435 Ubiquitination
K452 Ubiquitination
T453 Phosphorylation
Y469 Phosphorylation
Y511 Phosphorylation
Y549 Phosphorylation
T561 Phosphorylation
T563 Phosphorylation
S581 Phosphorylation
Y636 Phosphorylation
Y638 Phosphorylation
Y648 Phosphorylation
K676 Ubiquitination
K683 Ubiquitination
Y717 Phosphorylation
S726 Phosphorylation
S729 Phosphorylation
S735 Phosphorylation
S758 Phosphorylation
K816 Ubiquitination
K836 Ubiquitination
S934 Phosphorylation
T943 Phosphorylation
K965 Ubiquitination
S972 Phosphorylation
T976 Phosphorylation
S978 Phosphorylation
S981 Phosphorylation
S985 Phosphorylation
K1006 Ubiquitination
S1007 Phosphorylation
S1018 Phosphorylation
S1020 Phosphorylation
S1033 Phosphorylation
S1034 Phosphorylation
S1036 Phosphorylation
S1049 Phosphorylation
T1135 Phosphorylation
S1144 Phosphorylation

Research Backgrounds

Function:

Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.

PTMs:

Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated.

Subcellular Location:

Cell membrane>Lipid-anchor>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Muscle specific. Isoform 1 is predominant in vastus lateralis muscle. Isoform 2 predominates slightly in heart, and it predominates clearly in the other tissues tested.

Subunit Structure:

Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.

Family&Domains:

Belongs to the phosphorylase b kinase regulatory chain family.

Function:

Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.

PTMs:

Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated.

Subcellular Location:

Cell membrane>Lipid-anchor>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Predominantly expressed in liver and other non-muscle tissues.

Subunit Structure:

Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.

Family&Domains:

Belongs to the phosphorylase b kinase regulatory chain family.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

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