PDE10A Antibody - #AF9150
| Product: | PDE10A Antibody |
| Catalog: | AF9150 |
| Description: | Rabbit polyclonal antibody to PDE10A |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 75kDa; 88kD(Calculated). |
| Uniprot: | Q9Y233 |
| RRID: | AB_2843340 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF9150, RRID:AB_2843340.
Fold/Unfold
5''-cyclic phosphodiesterase 10A; cAMP and cAMP inhibited cGMP 3'5' cyclic phosphodiesterase 10A; cAMP and cAMP-inhibited cGMP 3''; FLJ11894; HSPDE 10A; HSPDE10A; PDE 10A; PDE10_HUMAN; Pde10a; Phosphodiesterase 10A; Phosphodiesterase 10A1;
Immunogens
Abundant in the putamen and caudate nucleus regions of brain and testis, moderately expressed in the thyroid gland, pituitary gland, thalamus and cerebellum.
- Q9Y233 PDE10_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRIEERKSQHLTGLTDEKVKAYLSLHPQVLDEFVSESVSAETVEKWLKRKNNKSEDESAPKEVSRYQDTNMQGVVYELNSYIEQRLDTGGDNQLLLYELSSIIKIATKADGFALYFLGECNNSLCIFTPPGIKEGKPRLIPAGPITQGTTVSAYVAKSRKTLLVEDILGDERFPRGTGLESGTRIQSVLCLPIVTAIGDLIGILELYRHWGKEAFCLSHQEVATANLAWASVAIHQVQVCRGLAKQTELNDFLLDVSKTYFDNIVAIDSLLEHIMIYAKNLVNADRCALFQVDHKNKELYSDLFDIGEEKEGKPVFKKTKEIRFSIEKGIAGQVARTGEVLNIPDAYADPRFNREVDLYTGYTTRNILCMPIVSRGSVIGVVQMVNKISGSAFSKTDENNFKMFAVFCALALHCANMYHRIRHSECIYRVTMEKLSYHSICTSEEWQGLMQFTLPVRLCKEIELFHFDIGPFENMWPGIFVYMVHRSCGTSCFELEKLCRFIMSVKKNYRRVPYHNWKHAVTVAHCMYAILQNNHTLFTDLERKGLLIACLCHDLDHRGFSNSYLQKFDHPLAALYSTSTMEQHHFSQTVSILQLEGHNIFSTLSSSEYEQVLEIIRKAIIATDLALYFGNRKQLEEMYQTGSLNLNNQSHRDRVIGLMMTACDLCSVTKLWPVTKLTANDIYAEFWAEGDEMKKLGIQPIPMMDRDKKDEVPQGQLGFYNAVAIPCYTTLTQILPPTEPLLKACRDNLSQWEKVIRGEETATWISSPSVAQKAAASED
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y233 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S8 | Phosphorylation | Uniprot | |
| T15 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| T88 | Phosphorylation | Uniprot | |
| K297 | Ubiquitination | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| T363 | Phosphorylation | Uniprot | |
| K395 | Ubiquitination | Uniprot | |
| Y639 | Phosphorylation | Uniprot | |
| T661 | Phosphorylation | Uniprot | |
| T669 | Phosphorylation | Uniprot | |
| K754 | Ubiquitination | Uniprot | |
| S767 | Phosphorylation | Uniprot |
Research Backgrounds
Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate. May play a critical role in regulating cAMP and cGMP levels in the striatum, a region of the brain that contributes to the control of movement and cognition.
Phosphorylated on Thr-16.
Cytoplasm.
Note: Located mostly to soluble cellular fractions.
Abundant in the putamen and caudate nucleus regions of brain and testis, moderately expressed in the thyroid gland, pituitary gland, thalamus and cerebellum.
Homodimer.
The tandem GAF domains bind cAMP, and regulate enzyme activity. The binding of cAMP stimulates enzyme activity.
Composed of a C-terminal catalytic domain containing two divalent metal sites and an N-terminal regulatory domain which contains one cyclic nucleotide-binding region.
Belongs to the cyclic nucleotide phosphodiesterase family.
Research Fields
· Human Diseases > Substance dependence > Morphine addiction.
· Metabolism > Nucleotide metabolism > Purine metabolism.
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