CD171/N-CAML1 Antibody - #AF9123

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Product: CD171/N-CAML1 Antibody
Catalog: AF9123
Description: Rabbit polyclonal antibody to CD171/N-CAML1
Application: WB IF/ICC
Reactivity: Human
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 180kDa; 140kD(Calculated).
Uniprot: P32004
RRID: AB_2843313

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
CD171/N-CAML1 Antibody detects endogenous levels of total CD171/N-CAML1.
RRID:
AB_2843313
Cite Format: Affinity Biosciences Cat# AF9123, RRID:AB_2843313.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Antigen identified by monoclonal antibody R1; CAML1; CD171; CD171 antigen; HSAS; HSAS1; Hyd; L1; L1 cell adhesion molecule; L1-NCAM; L1cam; L1CAM_HUMAN; MASA; MIC5; N CAML1; N-CAM-L1; NCAM-L1; NCAML1; Nerve-growth factor-inducible large external glycoprotein; Neural cell adhesion molecule L1; NILE; OTTHUMP00000025992; S10; SPG1;

Immunogens

Immunogen:
Uniprot:

P32004(L1CAM_HUMAN) >>Visit HPA database.

Gene(ID):
L1CAM(3897)
Sequence:
MVVALRYVWPLLLCSPCLLIQIPEEYEGHHVMEPPVITEQSPRRLVVFPTDDISLKCEASGKPEVQFRWTRDGVHFKPKEELGVTVYQSPHSGSFTITGNNSNFAQRFQGIYRCFASNKLGTAMSHEIRLMAEGAPKWPKETVKPVEVEEGESVVLPCNPPPSAEPLRIYWMNSKILHIKQDERVTMGQNGNLYFANVLTSDNHSDYICHAHFPGTRTIIQKEPIDLRVKATNSMIDRKPRLLFPTNSSSHLVALQGQPLVLECIAEGFPTPTIKWLRPSGPMPADRVTYQNHNKTLQLLKVGEEDDGEYRCLAENSLGSARHAYYVTVEAAPYWLHKPQSHLYGPGETARLDCQVQGRPQPEVTWRINGIPVEELAKDQKYRIQRGALILSNVQPSDTMVTQCEARNRHGLLLANAYIYVVQLPAKILTADNQTYMAVQGSTAYLLCKAFGAPVPSVQWLDEDGTTVLQDERFFPYANGTLGIRDLQANDTGRYFCLAANDQNNVTIMANLKVKDATQITQGPRSTIEKKGSRVTFTCQASFDPSLQPSITWRGDGRDLQELGDSDKYFIEDGRLVIHSLDYSDQGNYSCVASTELDVVESRAQLLVVGSPGPVPRLVLSDLHLLTQSQVRVSWSPAEDHNAPIEKYDIEFEDKEMAPEKWYSLGKVPGNQTSTTLKLSPYVHYTFRVTAINKYGPGEPSPVSETVVTPEAAPEKNPVDVKGEGNETTNMVITWKPLRWMDWNAPQVQYRVQWRPQGTRGPWQEQIVSDPFLVVSNTSTFVPYEIKVQAVNSQGKGPEPQVTIGYSGEDYPQAIPELEGIEILNSSAVLVKWRPVDLAQVKGHLRGYNVTYWREGSQRKHSKRHIHKDHVVVPANTTSVILSGLRPYSSYHLEVQAFNGRGSGPASEFTFSTPEGVPGHPEALHLECQSNTSLLLRWQPPLSHNGVLTGYVLSYHPLDEGGKGQLSFNLRDPELRTHNLTDLSPHLRYRFQLQATTKEGPGEAIVREGGTMALSGISDFGNISATAGENYSVVSWVPKEGQCNFRFHILFKALGEEKGGASLSPQYVSYNQSSYTQWDLQPDTDYEIHLFKERMFRHQMAVKTNGTGRVRLPPAGFATEGWFIGFVSAIILLLLVLLILCFIKRSKGGKYSVKDKEDTQVDSEARPMKDETFGEYRSLESDNEEKAFGSSQPSLNGDIKPLGSDDSLADYGGSVDVQFNEDGSFIGQYSGKKEKEAAGGNDSSGATSPINPAVALE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P32004 As Substrate

Site PTM Type Enzyme
Phosphorylation
K56 Ubiquitination
K77 Ubiquitination
K140 Ubiquitination
K144 Ubiquitination
S153 Phosphorylation
S163 Phosphorylation
K180 Ubiquitination
K222 Ubiquitination
K230 Ubiquitination
K295 Ubiquitination
K301 Ubiquitination
K338 Ubiquitination
K378 Ubiquitination
Y418 Phosphorylation
Y420 Phosphorylation
K515 Ubiquitination
T518 Phosphorylation
T521 Phosphorylation
T527 Phosphorylation
K530 Acetylation
K568 Ubiquitination
S611 Phosphorylation
K655 Ubiquitination
K661 Ubiquitination
K667 Ubiquitination
N671 N-Glycosylation
K694 Ubiquitination
K716 Ubiquitination
Y784 Phosphorylation
K998 Ubiquitination
K1150 Ubiquitination
Y1151 Phosphorylation
S1152 Phosphorylation
K1154 Ubiquitination
K1156 Ubiquitination
S1163 Phosphorylation
K1169 Ubiquitination
T1172 Phosphorylation P68400 (CSNK2A1) , P17252 (PRKCA)
Y1176 Phosphorylation P12931 (SRC)
S1181 Phosphorylation P68400 (CSNK2A1)
S1190 Phosphorylation
S1191 Phosphorylation
S1194 Phosphorylation
S1204 Phosphorylation P28482 (MAPK1)
Y1211 Phosphorylation
Y1229 Phosphorylation P54753 (EPHB3)
K1235 Ubiquitination
S1243 Phosphorylation
S1244 Phosphorylation
T1247 Phosphorylation
S1248 Phosphorylation P28482 (MAPK1)

Research Backgrounds

Function:

Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Cell projection>Growth cone. Cell projection>Axon. Cell projection>Dendrite.
Note: Colocalized with SHTN1 in close apposition with actin filaments in filopodia and lamellipodia of axonalne growth cones of hippocampal neurons (By similarity). In neurons, detected predominantly in axons and cell body, weak localization to dendrites (PubMed:20621658).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with SHTN1; the interaction occurs in axonal growth cones.

Family&Domains:

Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

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