MRLC1/MRLC2 Antibody - #AF9118
| Product: | MRLC1/MRLC2 Antibody |
| Catalog: | AF9118 |
| Description: | Rabbit polyclonal antibody to MRLC1/MRLC2 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 19kDa; 20kD(Calculated). |
| Uniprot: | P24844 | O14950 |
| RRID: | AB_2843308 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF9118, RRID:AB_2843308.
Fold/Unfold
P24844; 20 kDa myosin light chain;Human 20kDa myosin light chain (MLC2) mRNA complete cds;LC20;MGC3505;MLC 2;MLC-2C;MLC2;MLY 9;MRLC1;MYL9;MYL9_HUMAN;Myosin light chain 9 regulatory;Myosin light polypeptide 9 regulatory;myosin regulatory light chain 1;Myosin regulatory light chain 2;Myosin regulatory light chain 2 smooth muscle isoform;Myosin regulatory light chain 9;Myosin regulatory light chain MRLC1;Myosin regulatory light polypeptide 9;Myosin RLC;Myosin vascular smooth muscle light chain 2;MYRL2;OTTHUMP00000030857;smooth muscle isoform; O14950; ML12B_HUMAN;MLC 2A;MLC-2;MLC-2a;MLC-B;MLC20;MRLC2;MYL12B;MYLC2B;Myosin light chain 12B regulatory;Myosin regulatory light chain 12B;myosin regulatory light chain 2;Myosin regulatory light chain 2-B;myosin regulatory light chain 2-B, smooth muscle isoform;Myosin regulatory light chain 20 kDa;Myosin regulatory light chain MRLC2;myosin, light chain 12B, regulatory;OTTHUMP00000162244;OTTHUMP00000165806;OTTHUMP00000165807;OTTHUMP00000165808;SHUJUN-1;smooth muscle isoform;
Immunogens
Smooth muscle tissues and in some, but not all, nonmuscle cells.
O14950 ML12B_HUMAN:Ubiquitously expressed in various hematopoietic cells.
- P24844 MYL9_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSKRAKAKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDHLRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKDD
- O14950 ML12B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSKKAKTKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKDD
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P24844/O14950 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S3 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| T10 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| T11 | Phosphorylation | Uniprot | |
| T19 | Phosphorylation | O43293 (DAPK3) , O14578 (CIT) , Q15746 (MYLK) , Q5VT25 (CDC42BPA) , Q13464 (ROCK1) | Uniprot |
| S20 | Phosphorylation | O43293 (DAPK3) , Q5VT25 (CDC42BPA) , O75116 (ROCK2) , Q14012 (CAMK1) , Q15746 (MYLK) , Q13153 (PAK1) , Q13177 (PAK2) , Q9Y5S2 (CDC42BPB) , O14578 (CIT) , Q13464 (ROCK1) | Uniprot |
| S29 | Phosphorylation | Uniprot | |
| K51 | Acetylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| T97 | Phosphorylation | Uniprot | |
| S115 | Phosphorylation | Uniprot | |
| T128 | Phosphorylation | Uniprot | |
| T135 | Phosphorylation | Uniprot | |
| Y143 | Phosphorylation | P00533 (EGFR) | Uniprot |
| K150 | Ubiquitination | Uniprot | |
| K151 | Sumoylation | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| Y156 | Phosphorylation | P00533 (EGFR) | Uniprot |
| K164 | Ubiquitination | Uniprot | |
| K168 | Acetylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T10 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| T11 | Phosphorylation | Uniprot | |
| T19 | Phosphorylation | Q12852 (MAP3K12) , Q13418 (ILK) , O43293 (DAPK3) , P17252 (PRKCA) , Q13464 (ROCK1) , Q15746 (MYLK) | Uniprot |
| S20 | Phosphorylation | Q13464 (ROCK1) , Q13418 (ILK) , Q15746 (MYLK) , Q96GD4 (AURKB) , P17252 (PRKCA) , Q12852 (MAP3K12) , O43293 (DAPK3) , P53355 (DAPK1) | Uniprot |
| S29 | Phosphorylation | Uniprot | |
| K51 | Acetylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| T97 | Phosphorylation | Uniprot | |
| T128 | Phosphorylation | Uniprot | |
| R133 | Methylation | Uniprot | |
| T135 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| Y143 | Phosphorylation | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| K151 | Sumoylation | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| Y156 | Phosphorylation | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| K168 | Acetylation | Uniprot |
Research Backgrounds
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
Smooth muscle tissues and in some, but not all, nonmuscle cells.
Myosin is a hexamer of 2 heavy chains and 4 light chains: interacts with myosin heavy chain MYO19.
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion.
Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is reduced following epigallocatechin-3-O-gallate treatment.
Ubiquitously expressed in various hematopoietic cells.
Myosin is a hexamer of 2 heavy chains and 4 light chains: interacts with myosin heavy chain MYO19.
Research Fields
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)
· Organismal Systems > Immune system > Platelet activation. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
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