MINK1 Antibody - #AF9112

(3)  
Product: MINK1 Antibody
Catalog: AF9112
Description: Rabbit polyclonal antibody to MINK1
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Zebrafish, Bovine, Horse, Dog, Xenopus
Mol.Wt.: 150kDa; 150kD(Calculated).
Uniprot: Q8N4C8
RRID: AB_2843302

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
MINK1 Antibody detects endogenous levels of total MINK1.
RRID:
AB_2843302
Cite Format: Affinity Biosciences Cat# AF9112, RRID:AB_2843302.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

B55; GCK family kinase MiNK; hMINK; Map4k6; MAPK/ERK kinase kinase kinase 6; MEK (MAP/ERK) kinase kinase 6; MEK kinase kinase 6; MEKKK6; MGC21111; MINK 1; MINK; MINK1; Misshapen like kinase 1; Misshapen NIKs Related kinase; Misshapen/NIK related kinase; Mitogen activated protein kinase kinase kinase kinase 6; RP23-122P1.6; Ysk2; ZC3;

Immunogens

Immunogen:
Uniprot:

Q8N4C8(MINK1_HUMAN) >>Visit HPA database.

Gene(ID):
MINK1(50488)
Expression:
Q8N4C8 MINK1_HUMAN:

Expressed in the brain, isoform 2 is more abundant than isoform 1. Isoform 3 is ubiquitously expressed. Isoform 1 is most abundant in the skeletal muscle. Isoform 4 is ubiquitously expressed with relative high levels in brain, skeletal muscle, pancreas and testis.

Sequence:
MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQLLPGDRKPLYHYGRGMNPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAIPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGTPKPPGPPAQPPGPPNASSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGVSSKPDSSPVLSPGNKAKPDDHRSRPGRPADFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDDEEEGEGGPAEGSRDTPGGRSDGDTDSVSTMVVHDVEEITGTQPPYGGGTMVVQRTPEEERNLLHADSNGYTNLPDVVQPSHSPTENSKGQSPPSKDGSGDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDSIPITALVGGEGTRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
82
Chicken
67
Rabbit
64
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8N4C8 As Substrate

Site PTM Type Enzyme
S9 Phosphorylation
S16 Phosphorylation
Y36 Phosphorylation
K46 Ubiquitination
K54 Ubiquitination
T59 Phosphorylation
Y86 Phosphorylation
K155 Ubiquitination
S175 Phosphorylation
T181 Phosphorylation
T187 Phosphorylation Q8N4C8 (MINK1)
T191 Phosphorylation Q8N4C8 (MINK1)
Y193 Phosphorylation Q8N4C8 (MINK1)
T280 Phosphorylation O14757 (CHEK1)
K303 Ubiquitination
Y321 Phosphorylation
Y323 Phosphorylation
S324 Phosphorylation
S326 Phosphorylation
S332 Phosphorylation
K363 Ubiquitination
K370 Ubiquitination
K388 Ubiquitination
Y475 Phosphorylation
K477 Ubiquitination
S478 Phosphorylation
K490 Ubiquitination
Y505 Phosphorylation
Y507 Phosphorylation
R509 Methylation
S535 Phosphorylation
S563 Phosphorylation
T565 Phosphorylation
S581 Phosphorylation
K590 Acetylation
K590 Methylation
Y592 Phosphorylation
S601 Phosphorylation
T629 Phosphorylation
S641 Phosphorylation
T644 Phosphorylation
S673 Phosphorylation
S674 Phosphorylation
S699 Phosphorylation
S701 Phosphorylation
Y706 Phosphorylation
S732 Phosphorylation
S733 Phosphorylation
S740 Phosphorylation
S747 Phosphorylation
S749 Phosphorylation
S754 Phosphorylation
S763 Phosphorylation
S772 Phosphorylation
S773 Phosphorylation
S777 Phosphorylation
S778 Phosphorylation
S782 Phosphorylation
T809 Phosphorylation
S903 Phosphorylation
Y906 Phosphorylation
T907 Phosphorylation
S916 Phosphorylation
S918 Phosphorylation
T920 Phosphorylation
S927 Phosphorylation
K931 Ubiquitination
Y937 Phosphorylation
S939 Phosphorylation
Y960 Phosphorylation
Y987 Phosphorylation
S993 Phosphorylation
T1008 Phosphorylation
S1088 Phosphorylation
K1103 Ubiquitination
K1146 Ubiquitination
K1150 Ubiquitination
K1291 Ubiquitination
K1297 Ubiquitination
K1305 Ubiquitination
S1317 Phosphorylation
Y1320 Phosphorylation
T1323 Phosphorylation

Research Backgrounds

Function:

Serine/threonine kinase which acts as a negative regulator of Ras-related Rap2-mediated signal transduction to control neuronal structure and AMPA receptor trafficking. Required for normal synaptic density, dendrite complexity, as well as surface AMPA receptor expression in hippocampal neurons. Can activate the JNK and MAPK14/p38 pathways and mediates stimulation of the stress-activated protein kinase MAPK14/p38 MAPK downstream of the Raf/ERK pathway. Phosphorylates: TANC1 upon stimulation by RAP2A, MBP and SMAD1. Has an essential function in negative selection of thymocytes, perhaps by coupling NCK1 to activation of JNK1.

Isoform 4 can activate the JNK pathway. Involved in the regulation of actin cytoskeleton reorganization, cell-matrix adhesion, cell-cell adhesion and cell migration.

PTMs:

Autophosphorylated.

Subcellular Location:

Cytoplasm. Cell junction>Synapse>Postsynaptic density. Cell projection>Axon. Cell projection>Dendrite.

Golgi apparatus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the brain, isoform 2 is more abundant than isoform 1. Isoform 3 is ubiquitously expressed. Isoform 1 is most abundant in the skeletal muscle. Isoform 4 is ubiquitously expressed with relative high levels in brain, skeletal muscle, pancreas and testis.

Subunit Structure:

Interacts with TANC1 (By similarity). Interacts with RAP2A. Isoform 4 interacts with NCK1.

Family&Domains:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Restrictive clause

 

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