ME2 Antibody | Affinity Biosciences

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Western blot analysis of ME2 using RAW264.

AF9111 at 1/100 staining Human pancreatic cancer and adjacent nomal tissues by IHC-P.

AF9111 at 1/100 staining Human pancreatic cancer by IHC-P.

Product:	ME2 Antibody
Catalog:	AF9111
Description:	Rabbit polyclonal antibody to ME2
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	65kDa; 65kD(Calculated).
Uniprot:	P23368
RRID:	AB_2843301

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(82%), Bovine(82%), Horse(82%), Sheep(82%), Rabbit(82%), Dog(82%), Xenopus(91%)

Clonality:

Polyclonal

Specificity:

ME2 Antibody detects endogenous levels of total ME2.

RRID:

AB_2843301
Cite Format: Affinity Biosciences Cat# AF9111, RRID:AB_2843301.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Malate dehydrogenase; Malic enzyme 2; Malic enzyme 2 mitochondrial; Malic enzyme 2 NAD(+) dependent mitochondrial; Malic enzyme mitochondrial; Malic enzyme NAD(+) dependent mitochondrial; MAOM_HUMAN; ME 2; ME2; mitochondrial; NAD dependent malic enzyme mitochondrial; NAD ME; NAD-dependent malic enzyme; NAD-ME; ODS1; Pyruvic malic carboxylase;

Immunogens

Immunogen:

Uniprot:

P23368(MAOM_HUMAN) >>Visit HPA database.

Gene(ID):

ME2(4200)

Sequence:

P23368 MAOM_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLSRLRVVSTTCTLACRHLHIKEKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHRNLKKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLACSQYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIRPDRCLPVCIDVGTDNIALLKDPFYMGLYQKRDRTQQYDDLIDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYREKYCTFNDDIQGTAAVALAGLLAAQKVISKPISEHKILFLGAGEAALGIANLIVMSMVENGLSEQEAQKKIWMFDKYGLLVKGRKAKIDSYQEPFTHSAPESIPDTFEDAVNILKPSTIIGVAGAGRLFTPDVIRAMASINERPVIFALSNPTAQAECTAEEAYTLTEGRCLFASGSPFGPVKLTDGRVFTPGQGNNVYIFPGVALAVILCNTRHISDSVFLEAAKALTSQLTDEELAQGRLYPPLANIQEVSINIAIKVTEYLYANKMAFRYPEPEDKAKYVKERTWRSEYDSLLPDVYEWPESASSPPVITE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Xenopus

Pig

Horse

Bovine

Sheep

Dog

Rabbit

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P23368 As Substrate

P23368

Site	PTM Type	Source
K24	Acetylation	Uniprot
R67	Methylation	Uniprot
K94	Acetylation	Uniprot
K94	Ubiquitination	Uniprot
S106	Phosphorylation	Uniprot
K156	Acetylation	Uniprot
Y175	Phosphorylation	Uniprot
Y222	Phosphorylation	Uniprot
K224	Acetylation	Uniprot
K240	Acetylation	Uniprot
Y269	Phosphorylation	Uniprot
K272	Acetylation	Uniprot
T283	Phosphorylation	Uniprot
K339	Acetylation	Uniprot
K346	Acetylation	Uniprot
K346	Ubiquitination	Uniprot
Y347	Phosphorylation	Uniprot
T400	Phosphorylation	Uniprot
C441	S-Nitrosylation	Uniprot
K453	Ubiquitination	Uniprot
T503	Phosphorylation	Uniprot
Y533	Phosphorylation	Uniprot
K538	Ubiquitination	Uniprot
Y543	Phosphorylation	Uniprot

Research Backgrounds

P23368_MAOM_HUMAN

Subcellular Location:

Mitochondrion matrix.

Subunit Structure:

Homotetramer.

Family&Domains:

Belongs to the malic enzymes family.

Research Fields

· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.

· Metabolism > Global and overview maps > Carbon metabolism.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

ME2 Antibody - #AF9111