LHR Antibody - #AF9104

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Product: LHR Antibody
Catalog: AF9104
Description: Rabbit polyclonal antibody to LHR
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Dog
Mol.Wt.: 80kDa; 79kD(Calculated).
Uniprot: P22888
RRID: AB_2843294

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Dog(89%)
Clonality:
Polyclonal
Specificity:
LHR Antibody detects endogenous levels of total LHR.
RRID:
AB_2843294
Cite Format: Affinity Biosciences Cat# AF9104, RRID:AB_2843294.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Gonadotropin receptor; Gpcr19-rs1; GTHR-II; HHG; LCGR; LGR2; LH-R; LH/CG R; LH/CG-R; LH/CGR; LHCGR; LHR; LHRHR; LSH R; LSH-R; LSHR_HUMAN; Luteinizing hormone receptor; Luteinizing hormone/choriogonadotropin receptor; Lutropin choriogonadotropic hormone receptor; Lutropin choriogonadotropic receptor; Lutropin-choriogonadotropic hormone receptor; ULG5;

Immunogens

Immunogen:
Uniprot:

P22888(LSHR_HUMAN) >>Visit HPA database.

Gene(ID):
LHCGR(3973)
Expression:
P22888 LSHR_HUMAN:

Gonadal and thyroid cells.

Sequence:
MKQRFSALQLLKLLLLLQPPLPRALREALCPEPCNCVPDGALRCPGPTAGLTRLSLAYLPVKVIPSQAFRGLNEVIKIEISQIDSLERIEANAFDNLLNLSEILIQNTKNLRYIEPGAFINLPRLKYLSICNTGIRKFPDVTKVFSSESNFILEICDNLHITTIPGNAFQGMNNESVTLKLYGNGFEEVQSHAFNGTTLTSLELKENVHLEKMHNGAFRGATGPKTLDISSTKLQALPSYGLESIQRLIATSSYSLKKLPSRETFVNLLEATLTYPSHCCAFRNLPTKEQNFSHSISENFSKQCESTVRKVNNKTLYSSMLAESELSGWDYEYGFCLPKTPRCAPEPDAFNPCEDIMGYDFLRVLIWLINILAIMGNMTVLFVLLTSRYKLTVPRFLMCNLSFADFCMGLYLLLIASVDSQTKGQYYNHAIDWQTGSGCSTAGFFTVFASELSVYTLTVITLERWHTITYAIHLDQKLRLRHAILIMLGGWLFSSLIAMLPLVGVSNYMKVSICFPMDVETTLSQVYILTILILNVVAFFIICACYIKIYFAVRNPELMATNKDTKIAKKMAILIFTDFTCMAPISFFAISAAFKVPLITVTNSKVLLVLFYPINSCANPFLYAIFTKTFQRDFFLLLSKFGCCKRRAELYRRKDFSAYTSNCKNGFTGSNKPSQSTLKLSTLHCQGTALLDKTRYTEC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
89
Pig
78
Bovine
78
Sheep
78
Chicken
78
Rabbit
78
Xenopus
71
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P22888 As Substrate

Site PTM Type Enzyme
S494 Phosphorylation
S681 Phosphorylation
T688 Phosphorylation

Research Backgrounds

Function:

Receptor for lutropin-choriogonadotropic hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.

PTMs:

Sulfated.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Gonadal and thyroid cells.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

References

1). Study on the changes of LHR, FSHR and AR with the development of testis cells in Hu sheep. Animal Reproduction Science, 2023 (PubMed: 37541020) [IF=2.2]
2). miR-17-5p affects porcine granulosa cell growth and estradiol synthesis by targeting E2F1 gene. REPRODUCTION IN DOMESTIC ANIMALS, 2019 (PubMed: 31424586) [IF=1.7]

Application: WB    Species: porcine    Sample: porcine GCs

FIGURE 3 |miR‐17‐5p up‐regulates LHR, CYP19A1 and AREG expression and oestradiol synthesis in pGCs. All the experiments were done in porcine GCs 48 hr after transfected. (a) The mRNA expression levels of LHR, CYP19A1 and AREG in pGCs transfected with miR‐17‐5p mimics or mimics NC were detected by qRT‐PCR. (b) Protein levels of LHR, CYP19A1 and AREG in pGCs transfected with miR‐17‐5p mimics or mimics NC were detected by Western blot.

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