KIR3.4/KCNJ5 Antibody - #AF9102

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Product: KIR3.4/KCNJ5 Antibody
Catalog: AF9102
Description: Rabbit polyclonal antibody to KIR3.4/KCNJ5
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 48kDa; 48kD(Calculated).
Uniprot: P48544
RRID: AB_2843293

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(89%)
Clonality:
Polyclonal
Specificity:
KIR3.4/KCNJ5 Antibody detects endogenous levels of total KIR3.4/KCNJ5.
RRID:
AB_2843293
Cite Format: Affinity Biosciences Cat# AF9102, RRID:AB_2843293.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cardiac ATP sensitive potassium channel; Cardiac inward rectifier; CIR; G protein activated inward rectifier potassium channel 4; G protein-activated inward rectifier potassium channel 4; GIRK 4; GIRK-4; GIRK4; Heart KATP channel; Inward rectifier K(+) channel Kir3.4; Inward rectifier K+ channel KIR3.4; Inward rectifier potassium channel KIR3.4; inwardly rectifying subfamily J member 5; IRK-4; IRK5_HUMAN; KATP 1; KATP-1; KATP1; KCNJ 5; Kcnj5; KIR 3.4; KIR3.4; LQT13; Potassium channel; Potassium channel inwardly rectifying subfamily J member 5; Potassium inwardly rectifying channel J5; Potassium inwardly rectifying channel subfamily J member 5; potassium voltage-gated channel subfamily J member 5;

Immunogens

Immunogen:
Uniprot:

P48544(KCNJ5_HUMAN) >>Visit HPA database.

Gene(ID):
KCNJ5(3762)
Expression:
P48544 KCNJ5_HUMAN:

Islets, exocrine pancreas and heart. Expressed in the adrenal cortex, particularly the zona glomerulosa.

Sequence:
MAGDSRNAMNQDMEIGVTPWDPKKIPKQARDYVPIATDRTRLLAEGKKPRQRYMEKSGKCNVHHGNVQETYRYLSDLFTTLVDLKWRFNLLVFTMVYTVTWLFFGFIWWLIAYIRGDLDHVGDQEWIPCVENLSGFVSAFLFSIETETTIGYGFRVITEKCPEGIILLLVQAILGSIVNAFMVGCMFVKISQPKKRAETLMFSNNAVISMRDEKLCLMFRVGDLRNSHIVEASIRAKLIKSRQTKEGEFIPLNQTDINVGFDTGDDRLFLVSPLIISHEINQKSPFWEMSQAQLHQEEFEVVVILEGMVEATGMTCQARSSYMDTEVLWGHRFTPVLTLEKGFYEVDYNTFHDTYETNTPSCCAKELAEMKREGRLLQYLPSPPLLGGCAEAGLDAEAEQNEEDEPKGLGGSREARGSV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
89
Zebrafish
78
Xenopus
40
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P48544 As Substrate

Site PTM Type Enzyme
S75 Phosphorylation
T79 Phosphorylation
T199 Phosphorylation P17612 (PRKACA)
S203 Phosphorylation
S412 Phosphorylation P17612 (PRKACA)

Research Backgrounds

Function:

This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Islets, exocrine pancreas and heart. Expressed in the adrenal cortex, particularly the zona glomerulosa.

Subunit Structure:

May associate with GIRK1 and GIRK2 to form a G-protein-activated heteromultimer pore-forming unit. The resulting inward current is much larger.

Family&Domains:

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ5 subfamily.

Research Fields

· Human Diseases > Substance dependence > Morphine addiction.

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

References

1). Avaliação dos Canais de Potássio na Fisiopatologia da Pré-eclâmpsia. , 2022

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