InsP5 2-kinase Antibody | Affinity Biosciences

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Western blot analysis InsP5 2-kinase using K562 whole cell lysates.

Product:	InsP5 2-kinase Antibody
Catalog:	AF9097
Description:	Rabbit polyclonal antibody to InsP5 2-kinase
Application:	WB IHC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	56kDa; 56kD(Calculated).
Uniprot:	Q9H8X2
RRID:	AB_2843288

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(100%), Horse(91%), Sheep(100%), Rabbit(100%), Dog(91%)

Clonality:

Polyclonal

Specificity:

InsP5 2-kinase Antibody detects endogenous levels of total InsP5 2-kinase.

RRID:

AB_2843288
Cite Format: Affinity Biosciences Cat# AF9097, RRID:AB_2843288.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

3; 4; 5; 6)P5 2-kinase; 6-pentakisphosphate 2-kinase; bA476B13.1 (novel protein); bA476B13.1; C9orf12; inositol 1,3,4,5,6 pentakisphosphate 2 kinase; Inositol pentakisphosphate 2 kinase; Inositol-1; Inositol-pentakisphosphate 2-kinase; Ins; Ins(1; Ins(1,3,4,5,6)P5 2 kinase; InsP5 2 kinase; InsP5 2-kinase; INSP5K2; IP5K; IPK1; IPK1 homolog; IPPK; IPPK_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q9H8X2(IPPK_HUMAN) >>Visit HPA database.

Gene(ID):

IPPK(64768)

Expression:

Q9H8X2 IPPK_HUMAN:

Ubiquitously expressed, with high expression in heart, brain, testis and placenta.

Sequence:

Q9H8X2 IPPK_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEEGKMDENEWGYHGEGNKSLVVAHAQRCVVLRFLKFPPNRKKTSEEIFQHLQNIVDFGKNVMKEFLGENYVHYGEVVQLPLEFVKQLCLKIQSERPESRCDKDLDTLSGYAMCLPNLTRLQTYRFAEHRPILCVEIKPKCGFIPFSSDVTHEMKHKVCRYCMHQHLKVATGKWKQISKYCPLDLYSGNKQRMHFALKSLLQEAQNNLKIFKNGELIYGCKDARSPVADWSELAHHLKPFFFPSNGLASGPHCTRAVIRELVHVITRVLLSGSDKGRAGTLSPGLGPQGPRVCEASPFSRSLRCQGKNTPERSGLPKGCLLYKTLQVQMLDLLDIEGLYPLYNRVERYLEEFPEERKTLQIDGPYDEAFYQKLLDLSTEDDGTVAFALTKVQQYRVAMTAKDCSIMIALSPCLQDASSDQRPVVPSSRSRFAFSVSVLDLDLKPYESIPHQYKLDGKIVNYYSKTVRAKDNAVMSTRFKESEDCTLVLHKV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Rabbit

100

Horse

Dog

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H8X2 As Substrate

Q9H8X2

Site	PTM Type	Source
K173	Ubiquitination	Uniprot
K179	Ubiquitination	Uniprot
K190	Ubiquitination	Uniprot
K198	Methylation	Uniprot
K209	Ubiquitination	Uniprot
S282	Phosphorylation	Uniprot
K317	Ubiquitination	Uniprot
K357	Ubiquitination	Uniprot
S404	Phosphorylation	Uniprot
S418	Phosphorylation	Uniprot
K457	Ubiquitination	Uniprot
K479	Ubiquitination	Uniprot
K490	Ubiquitination	Uniprot

Research Backgrounds

Q9H8X2_IPPK_HUMAN

Function:

Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). InsP6 is involved in many processes such as mRNA export, non-homologous end-joining, endocytosis, ion channel regulation. It also protects cells from TNF-alpha-induced apoptosis.

Subcellular Location:

Cytoplasm. Nucleus.

Tissue Specificity:

Ubiquitously expressed, with high expression in heart, brain, testis and placenta.

Family&Domains:

The EXKPK motif is conserved in inositol-pentakisphosphate 2-kinases of both family 1 and 2.

Belongs to the IPK1 type 2 family.

Research Fields

· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.

· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

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For Research Use Only.
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InsP5 2-kinase Antibody - #AF9097