IL13 receptor alpha 1 Antibody | Affinity Biosciences

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Western blot analysis IL-13Ralpha1 using COLO205 whole cell lysates.

Product:	IL13 receptor alpha 1 Antibody
Catalog:	AF9095
Description:	Rabbit polyclonal antibody to IL13 receptor alpha 1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Horse, Sheep, Rabbit, Dog
Mol.Wt.:	49kDa; 49kD(Calculated).
Uniprot:	P78552
RRID:	AB_2843286

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Horse(89%), Sheep(89%), Rabbit(100%), Dog(89%)

Clonality:

Polyclonal

Specificity:

IL13 receptor alpha 1 Antibody detects endogenous levels of total IL13 receptor alpha 1.

RRID:

AB_2843286
Cite Format: Affinity Biosciences Cat# AF9095, RRID:AB_2843286.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

bB128O4.2.1 (interleukin 13 receptor, alpha 1); Cancer/testis antigen 19; CD213a1; CD213A1 Antigen; CT 19; CT19; I13R1_HUMAN; IL 13 receptor subunit alpha 1; IL 13R alpha 1; IL 13R subunit alpha 1; IL 13Ra; IL 13RA1; IL-13 receptor subunit alpha-1; IL-13R subunit alpha-1; IL-13R-alpha-1; IL-13RA1; IL13 receptor alpha 1 chain; IL13R; IL13RA; Il13ra1; Interleukin 13 receptor alpha 1; Interleukin 13 receptor alpha 1 chain; interleukin 13 receptor subunit alpha 1; Interleukin-13 receptor subunit alpha-1; NR 4; NR4;

Immunogens

Immunogen:

Uniprot:

P78552(I13R1_HUMAN) >>Visit HPA database.

Gene(ID):

IL13RA1(3597)

Expression:

P78552 I13R1_HUMAN:

Ubiquitous. Highest levels in heart, liver, skeletal muscle and ovary; lowest levels in brain, lung and kidney. Also found in B-cells, T-cells and endothelial cells.

Sequence:

P78552 I13R1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEWPARLCGLWALLLCAGGGGGGGGAAPTETQPPVTNLSVSVENLCTVIWTWNPPEGASSNCSLWYFSHFGDKQDKKIAPETRRSIEVPLNERICLQVGSQCSTNESEKPSILVEKCISPPEGDPESAVTELQCIWHNLSYMKCSWLPGRNTSPDTNYTLYYWHRSLEKIHQCENIFREGQYFGCSFDLTKVKDSSFEQHSVQIMVKDNAGKIKPSFNIVPLTSRVKPDPPHIKNLSFHNDDLYVQWENPQNFISRCLFYEVEVNNSQTETHNVFYVQEAKCENPEFERNVENTSCFMVPGVLPDTLNTVRIRVKTNKLCYEDDKLWSNWSQEMSIGKKRNSTLYITMLLIVPVIVAGAIIVLLLYLKRLKIIIFPPIPDPGKIFKEMFGDQNDDTLHWKKYDIYEKQTKEETDSVVLIENLKKASQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Horse

Sheep

Dog

Pig

Bovine

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P78552 As Substrate

P78552

Site	PTM Type	Source
T82	Phosphorylation	Uniprot
Y366	Phosphorylation	Uniprot
K386	Ubiquitination	Uniprot
K400	Ubiquitination	Uniprot
Y402	Phosphorylation	Uniprot
Y405	Phosphorylation	Uniprot
K407	Ubiquitination	Uniprot
K410	Ubiquitination	Uniprot
T413	Phosphorylation	Uniprot
S415	Phosphorylation	Uniprot
K423	Ubiquitination	Uniprot

Research Backgrounds

P78552_I13R1_HUMAN

Function:

Binds with low affinity to interleukin-13 (IL13). Together with IL4RA can form a functional receptor for IL13. Also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Tissue Specificity:

Ubiquitous. Highest levels in heart, liver, skeletal muscle and ovary; lowest levels in brain, lung and kidney. Also found in B-cells, T-cells and endothelial cells.

Subunit Structure:

Interleukin-13 receptor is a complex of IL4R, IL13RA1, and possibly other components. Interacts with TRAF3IP1.

Family&Domains:

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

The box 1 motif is required for JAK interaction and/or activation.

Belongs to the type I cytokine receptor family. Type 5 subfamily.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

References

1). miR-31 attenuates murine allergic rhinitis by suppressing interleukin-13-induced nasal epithelial inflammatory responses. Molecular Medicine Reports, 2021 (PubMed: 33179116) [IF=3.4]

Application: IHC Species: Mouse Sample: nasal mucosal tissues

Figure 4. Expression of IL-13Rα1, p-STAT6 and STAT6 in nasal mucosal tissues. IL-13Rα1, p-STAT6 and STAT6 expression was detected by immunohistochemical staining. Nasal mucosal tissues from different groups were subjected to single staining using IL-13Rα1, p-STAT6 and STAT6 antibodies. (A) Immunohistochemical staining image (original magnification, ×400). (B-D) Immunohistochemical staining scores. (E) Relative expression level of p-STAT6/STAT6. All data are presented as the median (IQR) (n=6/group). Differences among groups were compared using Kruskal-Wallis followed by a Dunn's post hoc test. *P<0.05. AR, allergic rhinitis; miR-31, microRNA-31; NC, negative control; Ctrl, control; IL-13Rα1, interleukin-13 receptor α1 chain; STAT6, signal transducer and activator of transcription 6; p-STAT6, phosphorylated-STAT6.

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IL13 receptor alpha 1 Antibody - #AF9095