Product: IL13 receptor alpha 1 Antibody
Catalog: AF9095
Description: Rabbit polyclonal antibody to IL13 receptor alpha 1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Horse, Sheep, Rabbit, Dog
Mol.Wt.: 49kDa; 49kD(Calculated).
Uniprot: P78552
RRID: AB_2843286

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(89%), Sheep(89%), Rabbit(100%), Dog(89%)
Clonality:
Polyclonal
Specificity:
IL13 receptor alpha 1 Antibody detects endogenous levels of total IL13 receptor alpha 1.
RRID:
AB_2843286
Cite Format: Affinity Biosciences Cat# AF9095, RRID:AB_2843286.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

bB128O4.2.1 (interleukin 13 receptor, alpha 1); Cancer/testis antigen 19; CD213a1; CD213A1 Antigen; CT 19; CT19; I13R1_HUMAN; IL 13 receptor subunit alpha 1; IL 13R alpha 1; IL 13R subunit alpha 1; IL 13Ra; IL 13RA1; IL-13 receptor subunit alpha-1; IL-13R subunit alpha-1; IL-13R-alpha-1; IL-13RA1; IL13 receptor alpha 1 chain; IL13R; IL13RA; Il13ra1; Interleukin 13 receptor alpha 1; Interleukin 13 receptor alpha 1 chain; interleukin 13 receptor subunit alpha 1; Interleukin-13 receptor subunit alpha-1; NR 4; NR4;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P78552 I13R1_HUMAN:

Ubiquitous. Highest levels in heart, liver, skeletal muscle and ovary; lowest levels in brain, lung and kidney. Also found in B-cells, T-cells and endothelial cells.

Sequence:
MEWPARLCGLWALLLCAGGGGGGGGAAPTETQPPVTNLSVSVENLCTVIWTWNPPEGASSNCSLWYFSHFGDKQDKKIAPETRRSIEVPLNERICLQVGSQCSTNESEKPSILVEKCISPPEGDPESAVTELQCIWHNLSYMKCSWLPGRNTSPDTNYTLYYWHRSLEKIHQCENIFREGQYFGCSFDLTKVKDSSFEQHSVQIMVKDNAGKIKPSFNIVPLTSRVKPDPPHIKNLSFHNDDLYVQWENPQNFISRCLFYEVEVNNSQTETHNVFYVQEAKCENPEFERNVENTSCFMVPGVLPDTLNTVRIRVKTNKLCYEDDKLWSNWSQEMSIGKKRNSTLYITMLLIVPVIVAGAIIVLLLYLKRLKIIIFPPIPDPGKIFKEMFGDQNDDTLHWKKYDIYEKQTKEETDSVVLIENLKKASQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
89
Sheep
89
Dog
89
Pig
0
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P78552 As Substrate

Site PTM Type Enzyme
T82 Phosphorylation
Y366 Phosphorylation
K386 Ubiquitination
K400 Ubiquitination
Y402 Phosphorylation
Y405 Phosphorylation
K407 Ubiquitination
K410 Ubiquitination
T413 Phosphorylation
S415 Phosphorylation
K423 Ubiquitination

Research Backgrounds

Function:

Binds with low affinity to interleukin-13 (IL13). Together with IL4RA can form a functional receptor for IL13. Also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Highest levels in heart, liver, skeletal muscle and ovary; lowest levels in brain, lung and kidney. Also found in B-cells, T-cells and endothelial cells.

Subunit Structure:

Interleukin-13 receptor is a complex of IL4R, IL13RA1, and possibly other components. Interacts with TRAF3IP1.

Family&Domains:

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

The box 1 motif is required for JAK interaction and/or activation.

Belongs to the type I cytokine receptor family. Type 5 subfamily.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

References

1). miR-31 attenuates murine allergic rhinitis by suppressing interleukin-13-induced nasal epithelial inflammatory responses. Molecular Medicine Reports, 2021 (PubMed: 33179116) [IF=3.4]

Application: IHC    Species: Mouse    Sample: nasal mucosal tissues

Figure 4. Expression of IL-13Rα1, p-STAT6 and STAT6 in nasal mucosal tissues. IL-13Rα1, p-STAT6 and STAT6 expression was detected by immunohistochemical staining. Nasal mucosal tissues from different groups were subjected to single staining using IL-13Rα1, p-STAT6 and STAT6 antibodies. (A) Immunohistochemical staining image (original magnification, ×400). (B-D) Immunohistochemical staining scores. (E) Relative expression level of p-STAT6/STAT6. All data are presented as the median (IQR) (n=6/group). Differences among groups were compared using Kruskal-Wallis followed by a Dunn's post hoc test. *P<0.05. AR, allergic rhinitis; miR-31, microRNA-31; NC, negative control; Ctrl, control; IL-13Rα1, interleukin-13 receptor α1 chain; STAT6, signal transducer and activator of transcription 6; p-STAT6, phosphorylated-STAT6.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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