Product: HC-II Antibody
Catalog: AF9084
Description: Rabbit polyclonal antibody to HC-II
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Rabbit, Dog
Mol.Wt.: 57kDa; 57kD(Calculated).
Uniprot: P05546
RRID: AB_2843275

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(88%), Horse(100%), Rabbit(88%), Dog(100%)
Clonality:
Polyclonal
Specificity:
HC-II Antibody detects endogenous levels of total HC-II.
RRID:
AB_2843275
Cite Format: Affinity Biosciences Cat# AF9084, RRID:AB_2843275.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

D22S673; HC II; HC-II; HC2; HCF2; HEP2_HUMAN; Heparin cofactor 2; Heparin cofactor II; HLS2; Leuserpin 2; LS2; Protease inhibitor leuserpin 2; Protease inhibitor leuserpin-2; serine (or cysteine) proteinase inhibitor, clade D (heparin cofactor), member 1; Serpin D1; serpin peptidase inhibitor, clade D (heparin cofactor), member 1; SERPIND1; THPH10;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P05546 HEP2_HUMAN:

Expressed predominantly in liver. Also present in plasma.

Sequence:
MKHSLNALLIFLIITSAWGGSKGPLDQLEKGGETAQSADPQWEQLNNKNLSMPLLPADFHKENTVTNDWIPEGEEDDDYLDLEKIFSEDDDYIDIVDSLSVSPTDSDVSAGNILQLFHGKSRIQRLNILNAKFAFNLYRVLKDQVNTFDNIFIAPVGISTAMGMISLGLKGETHEQVHSILHFKDFVNASSKYEITTIHNLFRKLTHRLFRRNFGYTLRSVNDLYIQKQFPILLDFKTKVREYYFAEAQIADFSDPAFISKTNNHIMKLTKGLIKDALENIDPATQMMILNCIYFKGSWVNKFPVEMTHNHNFRLNEREVVKVSMMQTKGNFLAANDQELDCDILQLEYVGGISMLIVVPHKMSGMKTLEAQLTPRVVERWQKSMTNRTREVLLPKFKLEKNYNLVESLKLMGIRMLFDKNGNMAGISDQRIAIDLFKHQGTITVNEEGTQATTVTTVGFMPLSTQVRFTVDRPFLFLIYEHRTSCLLFMGRVANPSRS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Bovine
88
Rabbit
88
Zebrafish
71
Sheep
63
Pig
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05546 As Substrate

Site PTM Type Enzyme
S37 Phosphorylation
N49 N-Glycosylation
T66 Phosphorylation
Y79 Phosphorylation
Y138 Phosphorylation
N188 N-Glycosylation
N387 N-Glycosylation

Research Backgrounds

Function:

Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.

Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils.

PTMs:

Phosphorylated by FAM20C in the extracellular medium.

Tissue Specificity:

Expressed predominantly in liver. Also present in plasma.

Family&Domains:

The N-terminal acidic repeat region mediates, in part, the glycosaminoglycan-accelerated thrombin inhibition.

Belongs to the serpin family.

Research Fields

· Organismal Systems > Immune system > Complement and coagulation cascades.   (View pathway)

References

1). Genistein treatment duration effects biomarkers of cell motility in human prostate. PLoS One, 2019 (PubMed: 30917169) [IF=3.7]

Application: WB    Species: human    Sample: human prostate cell

S5 Fig. | Effect of BASP1 and HCF2 knockdown and overexpression on respective transcript and protein levels.E. comparison of BASP1 and HCF2 protein expression in native cell lines measured by Western blot.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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