Glucosidase II alpha Antibody - #AF9072

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Product: Glucosidase II alpha Antibody
Catalog: AF9072
Description: Rabbit polyclonal antibody to Glucosidase II alpha
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 107kDa; 107kD(Calculated).
Uniprot: Q14697
RRID: AB_2843263

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(91%), Zebrafish(80%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(100%), Dog(91%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Glucosidase II alpha Antibody detects endogenous levels of total Glucosidase II alpha.
RRID:
AB_2843263
Cite Format: Affinity Biosciences Cat# AF9072, RRID:AB_2843263.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Alpha glucosidase II alpha subunit; Alpha-glucosidase 2; G2AN; GANAB; GANAB_HUMAN; Glu II; Glucosidase alpha neutral AB; Glucosidase II alpha; Glucosidase II subunit alpha; GluII; KIAA0088; Neutral alpha glucosidase AB; Neutral alpha glucosidase AB precursor; Neutral alpha-glucosidase AB;

Immunogens

Immunogen:
Uniprot:

Q14697(GANAB_HUMAN) >>Visit HPA database.

Gene(ID):
GANAB(23193)
Expression:
Q14697 GANAB_HUMAN:

Detected in placenta (PubMed:3881423). Isoform 1 and isoform 2 are expressed in the kidney and liver (PubMed:27259053).

Sequence:
MAAVAAVAARRRRSWASLVLAFLGVCLGITLAVDRSNFKTCEESSFCKRQRSIRPGLSPYRALLDSLQLGPDSLTVHLIHEVTKVLLVLELQGLQKNMTRFRIDELEPRRPRYRVPDVLVADPPIARLSVSGRDENSVELTMAEGPYKIILTARPFRLDLLEDRSLLLSVNARGLLEFEHQRAPRVSQGSKDPAEGDGAQPEETPRDGDKPEETQGKAEKDEPGAWEETFKTHSDSKPYGPMSVGLDFSLPGMEHVYGIPEHADNLRLKVTEGGEPYRLYNLDVFQYELYNPMALYGSVPVLLAHNPHRDLGIFWLNAAETWVDISSNTAGKTLFGKMMDYLQGSGETPQTDVRWMSETGIIDVFLLLGPSISDVFRQYASLTGTQALPPLFSLGYHQSRWNYRDEADVLEVDQGFDDHNLPCDVIWLDIEHADGKRYFTWDPSRFPQPRTMLERLASKRRKLVAIVDPHIKVDSGYRVHEELRNLGLYVKTRDGSDYEGWCWPGSAGYPDFTNPTMRAWWANMFSYDNYEGSAPNLFVWNDMNEPSVFNGPEVTMLKDAQHYGGWEHRDVHNIYGLYVHMATADGLRQRSGGMERPFVLARAFFAGSQRFGAVWTGDNTAEWDHLKISIPMCLSLGLVGLSFCGADVGGFFKNPEPELLVRWYQMGAYQPFFRAHAHLDTGRREPWLLPSQHNDIIRDALGQRYSLLPFWYTLLYQAHREGIPVMRPLWVQYPQDVTTFNIDDQYLLGDALLVHPVSDSGAHGVQVYLPGQGEVWYDIQSYQKHHGPQTLYLPVTLSSIPVFQRGGTIVPRWMRVRRSSECMKDDPITLFVALSPQGTAQGELFLDDGHTFNYQTRQEFLLRRFSFSGNTLVSSSADPEGHFETPIWIERVVIIGAGKPAAVVLQTKGSPESRLSFQHDPETSVLVLRKPGINVASDWSIHLR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Xenopus
100
Pig
91
Horse
91
Bovine
91
Sheep
91
Dog
91
Zebrafish
80
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14697 As Substrate

Site PTM Type Enzyme
S36 Phosphorylation
K39 Ubiquitination
T40 Phosphorylation
C47 S-Nitrosylation
K48 Acetylation
K48 Methylation
K48 Ubiquitination
S52 Phosphorylation
S58 Phosphorylation
Y60 Phosphorylation
N97 N-Glycosylation
T141 Phosphorylation
R164 Methylation
S165 Phosphorylation
R182 Methylation
S190 Phosphorylation
K191 Ubiquitination
T204 Phosphorylation
R206 Methylation
K217 Ubiquitination
K220 Ubiquitination
K231 Ubiquitination
K237 Ubiquitination
K269 Ubiquitination
K337 Ubiquitination
Y341 Phosphorylation
S345 Phosphorylation
T348 Phosphorylation
T351 Phosphorylation
Y379 Phosphorylation
Y396 Phosphorylation
K436 Acetylation
K462 Ubiquitination
K472 Sumoylation
K472 Ubiquitination
R484 Methylation
Y489 Phosphorylation
K491 Ubiquitination
Y563 Phosphorylation
Y578 Phosphorylation
R590 Methylation
S608 Phosphorylation
R698 Methylation
T790 Phosphorylation
Y792 Phosphorylation
T796 Phosphorylation
T808 Phosphorylation
S820 Phosphorylation
R863 Methylation
S866 Phosphorylation
S874 Phosphorylation
S876 Phosphorylation
K899 Ubiquitination
K908 Ubiquitination
K930 Ubiquitination
S937 Phosphorylation
R944 Methylation

Research Backgrounds

Function:

Catalytic subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. Required for PKD1/Polycystin-1 and PKD2/Polycystin-2 maturation and localization to the cell surface and cilia.

Subcellular Location:

Endoplasmic reticulum. Golgi apparatus. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in placenta. Isoform 1 and isoform 2 are expressed in the kidney and liver.

Subunit Structure:

Heterodimer of a catalytic alpha subunit (GANAB) and a beta subunit (PRKCSH). Binds glycosylated PTPRC (By similarity).

Family&Domains:

Belongs to the glycosyl hydrolase 31 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Metabolism > Glycan biosynthesis and metabolism > N-Glycan biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

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