eRF3/GSPT1 Antibody - #AF9062

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Product: eRF3/GSPT1 Antibody
Catalog: AF9062
Description: Rabbit polyclonal antibody to eRF3/GSPT1
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 55kDa; 56kD(Calculated).
Uniprot: P15170
RRID: AB_2843253

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
eRF3/GSPT1 Antibody detects endogenous levels of total eRF3/GSPT1.
RRID:
AB_2843253
Cite Format: Affinity Biosciences Cat# AF9062, RRID:AB_2843253.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

551G9.2; Anti Eukaryotic Release Factor 3a; Anti G1 to S phase transition 1; Anti GST1, homolog of yeast; eRF 3a; eRF3a; G1 to S phase transition 1; G1 to S phase transition protein 1 homolog; G1 to S phase transition protein; GSPT 1; GSPT1; GST 1; GST1; GTP binding protein GST1 HS; Transition protein 1;

Immunogens

Immunogen:
Uniprot:

P15170(ERF3A_HUMAN) >>Visit HPA database.

Gene(ID):
GSPT1(2935)
Sequence:
MELSEPIVENGETEMSPEESWEHKEEISEAEPGGGSLGDGRPPEESAHEMMEEEEEIPKPKSVVAPPGAPKKEHVNVVFIGHVDAGKSTIGGQIMYLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSNERYEECKEKLVPFLKKVGFNPKKDIHFMPCSGLTGANLKEQSDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVDKYKDMGTVVLGKLESGSICKGQQLVMMPNKHNVEVLGILSDDVETDTVAPGENLKIRLKGIEEEEILPGFILCDPNNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALICLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
82
Zebrafish
64
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P15170 As Substrate

Site PTM Type Enzyme
S16 Phosphorylation
S20 Phosphorylation
S36 Phosphorylation
S46 Phosphorylation
S62 Phosphorylation
K71 Ubiquitination
K108 Acetylation
K108 Ubiquitination
Y109 Phosphorylation
T120 Phosphorylation
T129 Phosphorylation
K138 Ubiquitination
Y146 Phosphorylation
K152 Ubiquitination
K187 Ubiquitination
T192 Phosphorylation
K196 Acetylation
K196 Methylation
K196 Ubiquitination
K208 Acetylation
K208 Ubiquitination
K238 Acetylation
K240 Ubiquitination
K253 Acetylation
K253 Ubiquitination
T315 Phosphorylation
K320 Ubiquitination
K328 Ubiquitination
K363 Ubiquitination
K367 Ubiquitination
K448 Ubiquitination
C453 S-Nitrosylation
K469 Acetylation
K469 Ubiquitination
T478 Phosphorylation
K484 Ubiquitination
K490 Acetylation
K490 Ubiquitination
K493 Acetylation
K493 Ubiquitination

Research Backgrounds

Function:

Involved in translation termination in response to the termination codons UAA, UAG and UGA (By similarity). Stimulates the activity of ETF1 (By similarity). Involved in regulation of mammalian cell growth. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes.

Subunit Structure:

Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. The ETF1-GSPT1 complex interacts with JMJD4. Interacts with PABPC1 (By similarity). Interacts with SHFL.

Family&Domains:

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. ERF3 subfamily.

Research Fields

· Genetic Information Processing > Translation > mRNA surveillance pathway.

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