Ephrin-B3 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of extracts from A2780 cells, using Ephrin-B3 Antibody.

AF9060 at 1/100 staining Mouse heart tissue by IHC-P.

Product:	Ephrin-B3 Antibody
Catalog:	AF9060
Description:	Rabbit polyclonal antibody to Ephrin-B3
Application:	WB IHC IF/ICC
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	36kDa; 36kD(Calculated).
Uniprot:	Q15768
RRID:	AB_2843251

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Ephrin-B3 Antibody detects endogenous levels of total Ephrin-B3.

RRID:

AB_2843251
Cite Format: Affinity Biosciences Cat# AF9060, RRID:AB_2843251.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

EFL 6; EFL6; EFNB 3; Efnb3; EFNB3_HUMAN; EPH related receptor transmembrane ligand ELK L3; EPH related receptor transmembrane ligand ELKL3; EPH related receptor tyrosine kinase ligand 8; EPH-related receptor transmembrane ligand ELK-L3; EPH-related receptor tyrosine kinase ligand 8; Ephrin B3; Ephrin-B3; EphrinB3; EPLG 8; EPLG8; LERK 8; LERK-8; LERK8;

Immunogens

Immunogen:

Uniprot:

Q15768(EFNB3_HUMAN) >>Visit HPA database.

Gene(ID):

EFNB3(1949)

Expression:

Q15768 EFNB3_HUMAN:

Highly expressed in brain; expressed in embryonic floor plate, roof plate and hindbrain segments.

Sequence:

Q15768 EFNB3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGPPHSGPGGVRVGALLLLGVLGLVSGLSLEPVYWNSANKRFQAEGGYVLYPQIGDRLDLLCPRARPPGPHSSPNYEFYKLYLVGGAQGRRCEAPPAPNLLLTCDRPDLDLRFTIKFQEYSPNLWGHEFRSHHDYYIIATSDGTREGLESLQGGVCLTRGMKVLLRVGQSPRGGAVPRKPVSEMPMERDRGAAHSLEPGKENLPGDPTSNATSRGAEGPLPPPSMPAVAGAAGGLALLLLGVAGAGGAMCWRRRRAKPSESRHPGPGSFGRGGSLGLGGGGGMGPREAEPGELGIALRGGGAADPPFCPHYEKVSGDYGHPVYIVQDGPPQSPPNIYYKV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15768 As Substrate

Q15768

Site	PTM Type	Source
S268	Phosphorylation	Uniprot
S274	Phosphorylation	Uniprot
Y311	Phosphorylation	Uniprot
Y318	Phosphorylation	Uniprot
Y323	Phosphorylation	Uniprot
S332	Phosphorylation	Uniprot
Y337	Phosphorylation	Uniprot
Y338	Phosphorylation	Uniprot

Research Backgrounds

Q15768_EFNB3_HUMAN

Function:

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity).

(Microbial infection) Acts as a receptor for nipah virus and hendra virus.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Tissue Specificity:

Highly expressed in brain; expressed in embryonic floor plate, roof plate and hindbrain segments.

Subunit Structure:

Interacts with GRIP1 and GRIP2.

(Microbial infection) Interacts with nipah virus and hendra virus glycoprotein.

Family&Domains:

Belongs to the ephrin family.

Research Fields

· Organismal Systems > Development > Axon guidance. (View pathway)

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Ephrin-B3 Antibody - #AF9060