DNA pol lambda Antibody - #AF9055

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Product: DNA pol lambda Antibody
Catalog: AF9055
Description: Rabbit polyclonal antibody to DNA pol lambda
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 63kDa; 63kD(Calculated).
Uniprot: Q9UGP5
RRID: AB_2843246

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
DNA pol lambda Antibody detects endogenous levels of total DNA pol lambda.
RRID:
AB_2843246
Cite Format: Affinity Biosciences Cat# AF9055, RRID:AB_2843246.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BETA N; BETAN; DNA directed DNA polymerase lambda; DNA polymerase beta 2; DNA polymerase beta-2; DNA polymerase kappa; DNA polymerase kappa DNA polymerase beta N; DNA polymerase lambda; DNA polymerase lamda2; DPOLL_HUMAN; EC 2.7.7.7,EC 4.2.99.; FLJ46002; OTTHUMP00000020321; OTTHUMP00000020323; OTTHUMP00000059179; Pol beta2; POL KAPPA; Pol Lambda; POLKAPPA; POLL; Polymerase DNA directed lambda;

Immunogens

Immunogen:
Uniprot:

Q9UGP5(DPOLL_HUMAN) >>Visit HPA database.

Gene(ID):
POLL(27343)
Expression:
Q9UGP5 DPOLL_HUMAN:

Expressed in a number of tissues. Abundant in testis.

Sequence:
MDPRGILKAFPKRQKIHADASSKVLAKIPRREEGEEAEEWLSSLRAHVVRTGIGRARAELFEKQIVQHGGQLCPAQGPGVTHIVVDEGMDYERALRLLRLPQLPPGAQLVKSAWLSLCLQERRLVDVAGFSIFIPSRYLDHPQPSKAEQDASIPPGTHEALLQTALSPPPPPTRPVSPPQKAKEAPNTQAQPISDDEASDGEETQVSAADLEALISGHYPTSLEGDCEPSPAPAVLDKWVCAQPSSQKATNHNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVTSYQEACSIPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKTAQMWYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQTVQKAAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLLDSLRQEGFLTDDLVSQEENGQQQKYLGVCRLPGPGRRHRRLDIIVVPYSEFACALLYFTGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHGCKVGPGRVLPTPTEKDVFRLLGLPYREPAERDW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
92
Zebrafish
92
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UGP5 As Substrate

Site PTM Type Enzyme
K23 Ubiquitination
K27 Ubiquitination
S43 Phosphorylation
S167 Phosphorylation P24941 (CDK2)
T173 Phosphorylation
S177 Phosphorylation P24941 (CDK2)
T204 Phosphorylation
S230 Phosphorylation P24941 (CDK2)
S246 Phosphorylation
K248 Ubiquitination
K273 Ubiquitination
S359 Phosphorylation
Y380 Phosphorylation
S381 Phosphorylation
T553 Phosphorylation P24941 (CDK2)

Research Backgrounds

Function:

DNA polymerase that functions in several pathways of DNA repair. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Also contributes to DNA double-strand break repair by non-homologous end joining and homologous recombination. Has both template-dependent and template-independent (terminal transferase) DNA polymerase activities. Has also a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in a number of tissues. Abundant in testis.

Subunit Structure:

Binds PCNA.

Family&Domains:

Belongs to the DNA polymerase type-X family.

Research Fields

· Genetic Information Processing > Replication and repair > Base excision repair.

· Genetic Information Processing > Replication and repair > Non-homologous end-joining.

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