DEF-3 Antibody - #AF9053

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Product: DEF-3 Antibody
Catalog: AF9053
Description: Rabbit polyclonal antibody to DEF-3
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 160kDa; 129kD(Calculated).
Uniprot: P78332
RRID: AB_2843244

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Bovine(92%), Horse(92%), Sheep(92%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
DEF-3 Antibody detects endogenous levels of total DEF-3.
RRID:
AB_2843244
Cite Format: Affinity Biosciences Cat# AF9053, RRID:AB_2843244.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

3G2; 4930506F14Rik; DEF 3; DEF3; DKFZp686B0877; FLJ36517; g16; HLC 11; KIAA4015; Lung cancer antigen NY LU 12; Lung cancer antigen NY-LU-12; Lung cancer protooncogene 11; mKIAA4015; NY LU 12; Protein G16; RBM6; RBM6/RBM5 fusion; RBM6_HUMAN; RGD1560367; RNA binding motif protein 6; RNA-binding motif protein 6; RNA-binding protein 6; RNA-binding protein DEF-3;

Immunogens

Immunogen:
Uniprot:

P78332(RBM6_HUMAN) >>Visit HPA database.

Gene(ID):
RBM6(10180)
Expression:
P78332 RBM6_HUMAN:

Ubiquitous in adults.

Sequence:
MWGDSRPANRTGPFRGSQEERFAPGWNRDYPPPPLKSHAQERHSGNFPGRDSLPFDFQGHSGPPFANVEEHSFSYGARDGPHGDYRGGEGPGHDFRGGDFSSSDFQSRDSSQLDFRGRDIHSGDFRDREGPPMDYRGGDGTSMDYRGREAPHMNYRDRDAHAVDFRGRDAPPSDFRGRGTYDLDFRGRDGSHADFRGRDLSDLDFRAREQSRSDFRNRDVSDLDFRDKDGTQVDFRGRGSGTTDLDFRDRDTPHSDFRGRHRSRTDQDFRGREMGSCMEFKDREMPPVDPNILDYIQPSTQDREHSGMNVNRREESTHDHTIERPAFGIQKGEFEHSETREGETQGVAFEHESPADFQNSQSPVQDQDKSQLSGREEQSSDAGLFKEEGGLDFLGRQDTDYRSMEYRDVDHRLPGSQMFGYGQSKSFPEGKTARDAQRDLQDQDYRTGPSEEKPSRLIRLSGVPEDATKEEILNAFRTPDGMPVKNLQLKEYNTGYDYGYVCVEFSLLEDAIGCMEANQGTLMIQDKEVTLEYVSSLDFWYCKRCKANIGGHRSSCSFCKNPREVTEAKQELITYPQPQKTSIPAPLEKQPNQPLRPADKEPEPRKREEGQESRLGHQKREAERYLPPSRREGPTFRRDRERESWSGETRQDGESKTIMLKRIYRSTPPEVIVEVLEPYVRLTTANVRIIKNRTGPMGHTYGFIDLDSHAEALRVVKILQNLDPPFSIDGKMVAVNLATGKRRNDSGDHSDHMHYYQGKKYFRDRRGGGRNSDWSSDTNRQGQQSSSDCYIYDSATGYYYDPLAGTYYDPNTQQEVYVPQDPGLPEEEEIKEKKPTSQGKSSSKKEMSKRDGKEKKDRGVTRFQENASEGKAPAEDVFKKPLPPTVKKEESPPPPKVVNPLIGLLGEYGGDSDYEEEEEEEQTPPPQPRTAQPQKREEQTKKENEEDKLTDWNKLACLLCRRQFPNKEVLIKHQQLSDLHKQNLEIHRKIKQSEQELAYLERREREGKFKGRGNDRREKLQSFDSPERKRIKYSRETDSDRKLVDKEDIDTSSKGGCVQQATGWRKGTGLGYGHPGLASSEEAEGRMRGPSVGASGRTSKRQSNETYRDAVRRVMFARYKELD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Dog
100
Horse
92
Bovine
92
Sheep
92
Chicken
75
Zebrafish
70
Pig
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P78332 As Substrate

Site PTM Type Enzyme
R15 Methylation
S17 Phosphorylation
K36 Methylation
S44 Phosphorylation
R96 Methylation
S111 Phosphorylation
S122 Phosphorylation
T141 Phosphorylation
R166 Methylation
R176 Methylation
R178 Methylation
S201 Phosphorylation
R238 Methylation
S240 Phosphorylation
T252 Phosphorylation
S255 Phosphorylation
R258 Methylation
S263 Phosphorylation
K331 Sumoylation
T344 Phosphorylation
S353 Phosphorylation
S360 Phosphorylation
S362 Phosphorylation
S370 Phosphorylation
S373 Phosphorylation
K386 Sumoylation
K386 Ubiquitination
T399 Phosphorylation
Y401 Phosphorylation
S403 Phosphorylation
Y406 Phosphorylation
K453 Sumoylation
S461 Phosphorylation
T468 Phosphorylation
K469 Sumoylation
K469 Ubiquitination
S554 Phosphorylation
S555 Phosphorylation
S557 Phosphorylation
K569 Sumoylation
Y701 Phosphorylation
K741 Sumoylation
K741 Ubiquitination
S746 Phosphorylation
S750 Phosphorylation
T836 Phosphorylation
S837 Phosphorylation
K840 Acetylation
K840 Ubiquitination
K844 Acetylation
K849 Acetylation
K871 Sumoylation
K887 Sumoylation
S891 Phosphorylation
Y908 Phosphorylation
S912 Phosphorylation
Y914 Phosphorylation
T923 Phosphorylation
K948 Sumoylation
K972 Acetylation
S977 Phosphorylation
K981 Acetylation
K991 Sumoylation
K1019 Ubiquitination
S1022 Phosphorylation
S1025 Phosphorylation
S1039 Phosphorylation
K1046 Sumoylation
T1051 Phosphorylation
S1052 Phosphorylation
S1053 Phosphorylation
T1062 Phosphorylation
K1066 Sumoylation

Research Backgrounds

Function:

Specifically binds poly(G) RNA homopolymers in vitro.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous in adults.

Subunit Structure:

May interact with FAM168B.

Restrictive clause

 

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