Product: CMTM4 Antibody
Catalog: AF9042
Description: Rabbit polyclonal antibody to CMTM4
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Zebrafish, Bovine, Chicken
Mol.Wt.: 25kDa; 26kD(Calculated).
Uniprot: Q8IZR5
RRID: AB_2843233

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
CMTM4 Antibody detects endogenous levels of total CMTM4.
RRID:
AB_2843233
Cite Format: Affinity Biosciences Cat# AF9042, RRID:AB_2843233.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Chemokine like factor super family 4; Chemokine-like factor superfamily member 4; CKLF-like MARVEL transmembrane domain containing 4; CKLF4; CKLFSF4; CMTM4;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q8IZR5 CKLF4_HUMAN:

Highly expressed in testis and prostate.

Sequence:
MRSGEELDGFEGEASSTSMISGASSPYQPTTEPVSQRRGLAGLRCDPDYLRGALGRLKVAQVILALIAFICIETIMACSPCEGLYFFEFVSCSAFVVTGVLLIMFSLNLHMRIPQINWNLTDLVNTGLSAFLFFIASIVLAALNHRAGAEIAAVIFGFLATAAYAVNTFLAVQKWRVSVRQQSTNDYIRARTESRDVDSRPEIQRLDTFSYSTNVTVRKKSPTNLLSLNHWQLA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Zebrafish
100
Chicken
100
Horse
0
Sheep
0
Dog
0
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IZR5 As Substrate

Site PTM Type Enzyme
S25 Phosphorylation
Y27 Phosphorylation
S183 Phosphorylation
Y187 Phosphorylation
S194 Phosphorylation
S199 Phosphorylation
T208 Phosphorylation

Research Backgrounds

Function:

Acts as a backup for CMTM6 to regulate plasma membrane expression of PD-L1/CD274, an immune inhibitory ligand critical for immune tolerance to self and antitumor immunity. May protect PD-L1/CD274 from being polyubiquitinated and targeted for degradation.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in testis and prostate.

Subunit Structure:

Interacts with PD-L1/CD274 and CMTM6.

Family&Domains:

Belongs to the chemokine-like factor family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.