Casein Kinase Igamma1 Antibody - #AF9033

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Product: Casein Kinase Igamma1 Antibody
Catalog: AF9033
Description: Rabbit polyclonal antibody to Casein Kinase Igamma1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 49kDa; 49kD(Calculated).
Uniprot: Q9HCP0
RRID: AB_2843224

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(83%), Zebrafish(92%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(83%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
Casein Kinase Igamma1 Antibody detects endogenous levels of total Casein Kinase Igamma1.
RRID:
AB_2843224
Cite Format: Affinity Biosciences Cat# AF9033, RRID:AB_2843224.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

9130020E21Rik; Casein kinase 1 gamma 1; Casein kinase I isoform gamma 1; Casein kinase I isoform gamma-1; CKI gamma 1; CKI-gamma 1; CSNK1G1; EC 2.7.11.1; KC1G1_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q9HCP0(KC1G1_HUMAN) >>Visit HPA database.

Gene(ID):
CSNK1G1(53944)
Sequence:
MDHPSREKDERQRTTKPMAQRSAHCSRPSGSSSSSGVLMVGPNFRVGKKIGCGNFGELRLGKNLYTNEYVAIKLEPIKSRAPQLHLEYRFYKQLGSAGEGLPQVYYFGPCGKYNAMVLELLGPSLEDLFDLCDRTFTLKTVLMIAIQLLSRMEYVHSKNLIYRDVKPENFLIGRQGNKKEHVIHIIDFGLAKEYIDPETKKHIPYREHKSLTGTARYMSINTHLGKEQSRRDDLEALGHMFMYFLRGSLPWQGLKADTLKERYQKIGDTKRNTPIEALCENFPEEMATYLRYVRRLDFFEKPDYEYLRTLFTDLFEKKGYTFDYAYDWVGRPIPTPVGSVHVDSGASAITRESHTHRDRPSQQQPLRNQVVSSTNGELNVDDPTGAHSNAPITAHAEVEVVEEAKCCCFFKRKRKKTAQRHK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Bovine
92
Sheep
92
Zebrafish
92
Pig
83
Xenopus
83
Chicken
83
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HCP0 As Substrate

Site PTM Type Enzyme
T14 Phosphorylation
T15 Phosphorylation
S22 Phosphorylation
S31 Phosphorylation
K49 Ubiquitination
K62 Ubiquitination
Y65 Phosphorylation
T66 Phosphorylation
Y69 Phosphorylation
Y88 Phosphorylation
Y91 Phosphorylation
S96 Phosphorylation
K166 Ubiquitination
Y194 Phosphorylation
K255 Ubiquitination
T258 Phosphorylation
K265 Ubiquitination
T309 Phosphorylation
K317 Ubiquitination
S344 Phosphorylation
S361 Phosphorylation
K411 Ubiquitination

PTMs - Q9HCP0 As Enzyme

Substrate Site Source
O75581 (LRP6) T1479 Uniprot
P25054 (APC) S1504 Uniprot
P25054 (APC) S1505 Uniprot
P25054 (APC) S1507 Uniprot
P25054 (APC) S1510 Uniprot
Q04206 (RELA) S536 Uniprot
Q12778 (FOXO1) S322 Uniprot

Research Backgrounds

Function:

Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Regulates fast synaptic transmission mediated by glutamate (By similarity). Phosphorylates CLSPN.

PTMs:

Autophosphorylated.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Monomer.

Family&Domains:

Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

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