ARRDC2 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of ARRDC2 using HeLa whole cell lysates.

Product:	ARRDC2 Antibody
Catalog:	AF9017
Description:	Rabbit polyclonal antibody to ARRDC2
Application:	WB IF/ICC
Reactivity:	Human
Mol.Wt.:	44kDa; 44kD(Calculated).
Uniprot:	Q8TBH0
RRID:	AB_2843208

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, WB 1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

ARRDC2 Antibody detects endogenous levels of total ARRDC2.

RRID:

AB_2843208
Cite Format: Affinity Biosciences Cat# AF9017, RRID:AB_2843208.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ARRDC2; Arrestin domain-containing protein 2; PP2703;

Immunogens

Immunogen:

Uniprot:

Q8TBH0(ARRD2_HUMAN) >>Visit HPA database.

Gene(ID):

ARRDC2(27106)

Sequence:

Q8TBH0 ARRD2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLFDKVKAFSVQLDGATAGVEPVFSGGQAVAGRVLLELSSAARVGALRLRARGRAHVHWTESRSAGSSTAYTQSYSERVEVVSHRATLLAPDTGETTTLPPGRHEFLFSFQLPPTLVTSFEGKHGSVRYCIKATLHRPWVPARRARKVFTVIEPVDINTPALLAPQAGAREKVARSWYCNRGLVSLSAKIDRKGYTPGEVIPVFAEIDNGSTRPVLPRAAVVQTQTFMARGARKQKRAVVASLAGEPVGPGQRALWQGRALRIPPVGPSILHCRVLHVDYALKVCVDIPGTSKLLLELPLVIGTIPLHPFGSRSSSVGSHASFLLDWRLGALPERPEAPPEYSEVVADTEEAALGQSPFPLPQDPDMSLEGPFFAYIQEFRYRPPPLYSEEDPNPLLGDMRPRCMTC

PTMs - Q8TBH0 As Substrate

Q8TBH0

Site	PTM Type	Source
S10	Phosphorylation	Uniprot
S25	Phosphorylation	Uniprot
S40	Phosphorylation	Uniprot
T96	Phosphorylation	Uniprot
T115	Phosphorylation	Uniprot
T118	Phosphorylation	Uniprot
S119	Phosphorylation	Uniprot
K147	Ubiquitination	Uniprot
K189	Ubiquitination	Uniprot
K193	Ubiquitination	Uniprot

Research Backgrounds

Q8TBH0_ARRD2_HUMAN

Subunit Structure:

Interacts with WWP1 (via WW domains).

Family&Domains:

Belongs to the arrestin family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

ARRDC2 Antibody - #AF9017