UBE4B Antibody | Affinity Biosciences

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Western blot analysis of extracts from EC304 cells, using UBE4B Antibody.

Product:	UBE4B Antibody
Catalog:	DF9997
Description:	Rabbit polyclonal antibody to UBE4B
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	146 kDa; 146kD(Calculated).
Uniprot:	O95155
RRID:	AB_2843189

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Zebrafish(86%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(92%), Dog(92%), Chicken(86%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

UBE4B Antibody detects endogenous levels of total UBE4B.

RRID:

AB_2843189
Cite Format: Affinity Biosciences Cat# DF9997, RRID:AB_2843189.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

4930551I19Rik; 4933406G05Rik; AU014668; D4Bwg0973e; E4; HDNB1; Homozygously deleted in neuroblastoma 1; KIAA0684; mKIAA0684; RP23 173D8.1; Ube4b; UBE4B_HUMAN; Ubiquitin conjugation factor E4 B; Ubiquitin fusion degradation protein 2; UBOX3; UFD2; UFD2a; Ufd2p;

Immunogens

Immunogen:

Uniprot:

O95155(UBE4B_HUMAN) >>Visit HPA database.

Gene(ID):

UBE4B(10277)

Expression:

O95155 UBE4B_HUMAN:

Expressed in differentiated myotubes (at protein level) (PubMed:17369820). Highest expression in ovary, testis, heart and skeletal muscle (PubMed:11802788). Expression is low in colon, thymus and peripheral blood leukocytes (PubMed:11802788). Almost undetectable in lung and spleen (PubMed:11802788).

Sequence:

O95155 UBE4B_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPPGPPIAASAPGPSQSLGLNVHNMTPATSPIGASGVAHRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDGVSCEKSMSQVDVDSGIENMEVDENDRREKRSLSDKEPSSGPEVSEEQALQLVCKIFRVSWKDRDRDVIFLSSLSAQFKQNPKEVFSDFKDLIGQILMEVLMMSTQTRDENPFASLTATSQPIAAAARSPDRNLLLNTGSNPGTSPMFCSVASFGASSLSSLYESSPAPTPSFWSSVPVMGPSLASPSRAASQLAVPSTPLSPHSAASGTAAGSQPSSPRYRPYTVTHPWASSGVSILSSSPSPPALASSPQAVPASSSRQRPSSTGPPLPPASPSATSRRPSSLRISPSLGASGGASNWDSYSDHFTIETCKETDMLNYLIECFDRVGIEEKKAPKMCSQPAVSQLLSNIRSQCISHTALVLQGSLTQPRSLQQPSFLVPYMLCRNLPYGFIQELVRTTHQDEEVFKQIFIPILQGLALAAKECSLDSDYFKYPLMALGELCETKFGKTHPVCNLVASLRLWLPKSLSPGCGRELQRLSYLGAFFSFSVFAEDDVKVVEKYFSGPAITLENTRVVSQSLQHYLELGRQELFKILHSILLNGETREAALSYMAAVVNANMKKAQMQTDDRLVSTDGFMLNFLWVLQQLSTKIKLETVDPTYIFHPRCRITLPNDETRVNATMEDVNDWLTELYGDQPPFSEPKFPTECFFLTLHAHHLSILPSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHREMLKRCKTQLKKLVRCKACADAGLLDESFLRRCLNFYGLLIQLLLRILDPAYPDITLPLNSDVPKVFAALPEFYVEDVAEFLFFIVQYSPQALYEPCTQDIVMFLVVMLCNQNYIRNPYLVAKLVEVMFMTNPAVQPRTQKFFEMIENHPLSTKLLVPSLMKFYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEVQEEMKNKEQWDQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHILTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCARFAKAIADDQRSYSKELFEEVISKMRKAGIKSTIAIEKFKLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPSGTIMDRSIILRHLLNSPTDPFNRQTLTESMLEPVPELKEQIQAWMREKQNSDH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Xenopus

100

Dog

Rabbit

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95155 As Substrate

O95155

Site	PTM Type	Source
M1	Acetylation	Uniprot
R10	Methylation	Uniprot
T22	Phosphorylation	Uniprot
S23	Phosphorylation	Uniprot
T26	Phosphorylation	Uniprot
T27	Phosphorylation	Uniprot
T30	Phosphorylation	Uniprot
S31	Phosphorylation	Uniprot
T61	Phosphorylation	Uniprot
T64	Phosphorylation	Uniprot
S65	Phosphorylation	Uniprot
S76	Phosphorylation	Uniprot
S78	Phosphorylation	Uniprot
S79	Phosphorylation	Uniprot
S83	Phosphorylation	Uniprot
S84	Phosphorylation	Uniprot
S86	Phosphorylation	Uniprot
S87	Phosphorylation	Uniprot
S88	Phosphorylation	Uniprot
S90	Phosphorylation	Uniprot
S92	Phosphorylation	Uniprot
T95	Phosphorylation	Uniprot
S99	Phosphorylation	Uniprot
S101	Phosphorylation	Uniprot
S103	Phosphorylation	Uniprot
S105	Phosphorylation	Uniprot
C113	S-Nitrosylation	Uniprot
S116	Phosphorylation	Uniprot
S118	Phosphorylation	Uniprot
S124	Phosphorylation	Uniprot
S141	Phosphorylation	Uniprot
S143	Phosphorylation	Uniprot
K145	Ubiquitination	Uniprot
S149	Phosphorylation	Uniprot
S154	Phosphorylation	Uniprot
K192	Ubiquitination	Uniprot
S224	Phosphorylation	Uniprot
S238	Phosphorylation	Uniprot
S307	Phosphorylation	Uniprot
S352	Phosphorylation	Uniprot
S383	Phosphorylation	Uniprot
S392	Phosphorylation	Uniprot
K442	Ubiquitination	Uniprot
K542	Ubiquitination	Uniprot
K558	Ubiquitination	Uniprot
K575	Ubiquitination	Uniprot
K610	Ubiquitination	Uniprot
K702	Ubiquitination	Uniprot
T705	Phosphorylation	Uniprot
T709	Phosphorylation	Uniprot
Y710	Phosphorylation	Uniprot
K794	Ubiquitination	Uniprot
S798	Phosphorylation	Uniprot
K801	Sumoylation	Uniprot
K801	Ubiquitination	Uniprot
S803	Phosphorylation	Uniprot
T807	Phosphorylation	Uniprot
K827	Ubiquitination	Uniprot
Y929	Phosphorylation	Uniprot
K951	Ubiquitination	Uniprot
K964	Ubiquitination	Uniprot
S969	Phosphorylation	Uniprot
K972	Ubiquitination	Uniprot
K989	Ubiquitination	Uniprot
Y1026	Phosphorylation	Uniprot
T1034	Phosphorylation	Uniprot
K1056	Ubiquitination	Uniprot
K1058	Ubiquitination	Uniprot
K1106	Ubiquitination	Uniprot
K1132	Ubiquitination	Uniprot
K1143	Ubiquitination	Uniprot
K1150	Ubiquitination	Uniprot
K1169	Ubiquitination	Uniprot
K1180	Ubiquitination	Uniprot
K1189	Ubiquitination	Uniprot
K1196	Ubiquitination	Uniprot
K1203	Ubiquitination	Uniprot
K1205	Ubiquitination	Uniprot
K1210	Ubiquitination	Uniprot
K1217	Ubiquitination	Uniprot
S1249	Phosphorylation	Uniprot
S1256	Phosphorylation	Uniprot
S1265	Phosphorylation	Uniprot
T1267	Phosphorylation	Uniprot
T1274	Phosphorylation	Uniprot
S1278	Phosphorylation	Uniprot

Research Backgrounds

O95155_UBE4B_HUMAN

Function:

Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases (By similarity). May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase (By similarity). May regulate myosin assembly in striated muscles together with STUB1 and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation.

PTMs:

Proteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7.

Subcellular Location:

Cytoplasm. Nucleus.

Tissue Specificity:

Expressed in differentiated myotubes (at protein level). Highest expression in ovary, testis, heart and skeletal muscle. Expression is low in colon, thymus and peripheral blood leukocytes. Almost undetectable in lung and spleen.

Subunit Structure:

Interacts with VCP/p97. Interacts with STUB1/CHIP and UNC45B.

Family&Domains:

The U-box domain is required for the ubiquitin protein ligase activity.

Belongs to the ubiquitin conjugation factor E4 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)

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UBE4B Antibody - #DF9997