LSM2 Antibody | Affinity Biosciences

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Western blot analysis of LSM2 using Jurkat whole cell lysates.

DF9979 at 1/100 staining mouse brain tissue by IHC-P.

DF9979 at 1/100 staining rat brain tissue by IHC-P.

Product:	LSM2 Antibody
Catalog:	DF9979
Description:	Rabbit polyclonal antibody to LSM2
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Xenopus
Mol.Wt.:	11 kDa; 11kD(Calculated).
Uniprot:	Q9Y333
RRID:	AB_2843171

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

LSM2 Antibody detects endogenous levels of total LSM2.

RRID:

AB_2843171
Cite Format: Affinity Biosciences Cat# DF9979, RRID:AB_2843171.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C6orf28; Chromosome 6 open reading frame 28; G7b; LSM 2; Lsm2; LSM2 homolog, U6 small nuclear RNA associated (S. cerevisiae); Lsm2 protein; LSM2_HUMAN; OTTHUMP00000029159; Protein G7b; Small nuclear ribonuclear protein D homolog; snRNP; snRNP core Sm like protein Sm x5; snRNP core Sm-like protein Sm-x5; U6 snRNA associated Sm like protein LSm2; U6 snRNA-associated Sm-like protein LSm2; YBL026W;

Immunogens

Immunogen:

Uniprot:

Q9Y333(LSM2_HUMAN) >>Visit HPA database.

Gene(ID):

LSM2(57819)

Sequence:

Q9Y333 LSM2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLFYSFFKSLVGKDVVVELKNDLSICGTLHSVDQYLNIKLTDISVTDPEKYPHMLSVKNCFIRGSVVRYVQLPADEVDTQLLQDAARKEALQQKQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Xenopus

100

Rabbit

100

Dog

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y333 As Substrate

Q9Y333

Site	PTM Type	Source
Y4	Phosphorylation	Uniprot
S5	Phosphorylation	Uniprot
K8	Ubiquitination	Uniprot
Y35	Phosphorylation	Uniprot
S44	Phosphorylation	Uniprot
T46	Phosphorylation	Uniprot
K50	Ubiquitination	Uniprot
Y51	Phosphorylation	Uniprot
S65	Phosphorylation	Uniprot
T79	Phosphorylation	Uniprot
K88	Ubiquitination	Uniprot
K94	Ubiquitination	Uniprot

Research Backgrounds

Q9Y333_LSM2_HUMAN

Function:

Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA.

Subcellular Location:

Nucleus.

Subunit Structure:

Component of the precatalytic spliceosome (spliceosome B complex). Component of the U4/U6-U5 tri-snRNP complex, a building block of the precatalytic spliceosome (spliceosome B complex). The U4/U6-U5 tri-snRNP complex is composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8. LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8 form a heptameric, ring-shaped subcomplex (the LSM2-8 complex) that is part of the U4/U6-U5 tri-snRNP complex and the precatalytic spliceosome.

Family&Domains:

Belongs to the snRNP Sm proteins family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

· Genetic Information Processing > Transcription > Spliceosome.

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LSM2 Antibody - #DF9979