SART1 Antibody - #DF9978

(2)  
Product: SART1 Antibody
Catalog: DF9978
Description: Rabbit polyclonal antibody to SART1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.: 90 kDa; 90kD(Calculated).
Uniprot: O43290
RRID: AB_2843170

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
SART1 Antibody detects endogenous levels of total SART1.
RRID:
AB_2843170
Cite Format: Affinity Biosciences Cat# DF9978, RRID:AB_2843170.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Ara 1; ARA1; HOMS 1; HOMS1; hSART 1; hSART1; hSnu 66; hSnu66; IgE autoantigen; MGC2038; SART 1; SART1 259; SART1(259) protein; SART1(800) protein; SART1259; Snu 66; Snu66; Squamous cell carcinoma antigen recognised by T cells; Squamous cell carcinoma antigen recognized by T cells 1; U4/U6.U5 tri snRNP associated 110 kDa protein; U4/U6.U5 tri snRNP associated protein 1;

Immunogens

Immunogen:
Uniprot:

O43290(SNUT1_HUMAN) >>Visit HPA database.

Gene(ID):
SART1(9092)
Expression:
O43290 SNUT1_HUMAN:

Ubiquitously expressed.

Sequence:
MGSSKKHRGEKEAAGTTAAAGTGGATEQPPRHREHKKHKHRSGGSGGSGGERRKRSRERGGERGSGRRGAEAEARSSTHGRERSQAEPSERRVKREKRDDGYEAAASSKTSSGDASSLSIEETNKLRAKLGLKPLEVNAIKKEAGTKEEPVTADVINPMALRQREELREKLAAAKEKRLLNQKLGKIKTLGEDDPWLDDTAAWIERSRQLQKEKDLAEKRAKLLEEMDQEFGVSTLVEEEFGQRRQDLYSARDLQGLTVEHAIDSFREGETMILTLKDKGVLQEEEDVLVNVNLVDKERAEKNVELRKKKPDYLPYAEDESVDDLAQQKPRSILSKYDEELEGERPHSFRLEQGGTADGLRERELEEIRAKLRLQAQSLSTVGPRLASEYLTPEEMVTFKKTKRRVKKIRKKEKEVVVRADDLLPLGDQTQDGDFGSRLRGRGRRRVSEVEEEKEPVPQPLPSDDTRVENMDISDEEEGGAPPPGSPQVLEEDEAELELQKQLEKGRRLRQLQQLQQLRDSGEKVVEIVKKLESRQRGWEEDEDPERKGAIVFNATSEFCRTLGEIPTYGLAGNREEQEELMDFERDEERSANGGSESDGEENIGWSTVNLDEEKQQQDFSASSTTILDEEPIVNRGLAAALLLCQNKGLLETTVQKVARVKAPNKSLPSAVYCIEDKMAIDDKYSRREEYRGFTQDFKEKDGYKPDVKIEYVDETGRKLTPKEAFRQLSHRFHGKGSGKMKTERRMKKLDEEALLKKMSSSDTPLGTVALLQEKQKAQKTPYIVLSGSGKSMNANTITK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Xenopus
83
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43290 As Substrate

Site PTM Type Enzyme
T22 Phosphorylation
S42 Phosphorylation
S45 Phosphorylation
S48 Phosphorylation
S84 Phosphorylation
K94 Sumoylation
Y102 Phosphorylation
S108 Phosphorylation
K109 Ubiquitination
S111 Phosphorylation
S112 Phosphorylation
S119 Phosphorylation
T123 Phosphorylation
K125 Ubiquitination
K129 Ubiquitination
K133 Acetylation
K133 Ubiquitination
K141 Sumoylation
K147 Sumoylation
K147 Ubiquitination
K183 Ubiquitination
K188 Sumoylation
K188 Ubiquitination
T189 Phosphorylation
T200 Phosphorylation
Y249 Phosphorylation
S250 Phosphorylation
T258 Phosphorylation
S265 Phosphorylation
T271 Phosphorylation
T275 Phosphorylation
K302 Ubiquitination
Y316 Phosphorylation
S321 Phosphorylation
K329 Ubiquitination
S332 Phosphorylation
S335 Phosphorylation
K336 Ubiquitination
S348 Phosphorylation
R385 Methylation
S388 Phosphorylation
Y390 Phosphorylation
T392 Phosphorylation
K400 Ubiquitination
T430 Phosphorylation
S437 Phosphorylation
S448 Phosphorylation
S463 Phosphorylation
T466 Phosphorylation
S474 Phosphorylation
S486 Phosphorylation
S521 Phosphorylation
K524 Ubiquitination
K530 Ubiquitination
S591 Phosphorylation
S596 Phosphorylation
S598 Phosphorylation
S621 Phosphorylation
S623 Phosphorylation
K648 Ubiquitination
K657 Ubiquitination
K666 Ubiquitination
S667 Phosphorylation
Y673 Phosphorylation
K678 Ubiquitination
K684 Ubiquitination
Y685 Phosphorylation
S686 Phosphorylation
R692 Methylation
T695 Phosphorylation
K699 Ubiquitination
K709 Sumoylation
Y712 Phosphorylation
K719 Ubiquitination
T721 Phosphorylation
K723 Ubiquitination
S730 Phosphorylation
K742 Sumoylation
K749 Ubiquitination
K757 Methylation
K758 Methylation
K758 Sumoylation
K758 Ubiquitination
S760 Phosphorylation
S761 Phosphorylation
S762 Phosphorylation
T764 Phosphorylation
T768 Phosphorylation
K775 Ubiquitination
T781 Phosphorylation
Y783 Phosphorylation
S789 Phosphorylation

Research Backgrounds

Function:

Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA.

PTMs:

Sumoylated with SUMO2.

Subcellular Location:

Nucleus.
Note: Found in the nucleus of mitogen-activated peripheral blood mononuclear cells (PBMCs), tumor cells, or normal cell lines, but not in normal tissues except testis and fetal liver or in unstimulated PBMCs, suggesting preferential expression in proliferating cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Identified in the spliceosome C complex. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39.

Family&Domains:

Belongs to the SNU66/SART1 family.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.