MPHOSPH10 Antibody - #DF9977

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Product: MPHOSPH10 Antibody
Catalog: DF9977
Description: Rabbit polyclonal antibody to MPHOSPH10
Application: WB
Reactivity: Human
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 20,70 kDa; 79kD(Calculated).
Uniprot: O00566
RRID: AB_2843169

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
MPHOSPH10 Antibody detects endogenous levels of total MPHOSPH10.
RRID:
AB_2843169
Cite Format: Affinity Biosciences Cat# DF9977, RRID:AB_2843169.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

M phase phosphoprotein 10 (U3 small nucleolar ribonucleoprotein); M phase phosphoprotein 10; MPHOSPH 10; Mphosph10; MPP 10; MPP 10P; MPP10; MPP10_HUMAN; MPP10P; U3 small nucleolar ribonucleoprotein protein mpp10;

Immunogens

Immunogen:
Uniprot:

O00566(MPP10_HUMAN) >>Visit HPA database.

Gene(ID):
MPHOSPH10(10199)
Sequence:
MAPQVWRRRTLERCLTEVGKATGRPECFLTIQEGLASKFTSLTKVLYDFNKILENGRIHGSPLQKLVIENFDDEQIWQQLELQNEPILQYFQNAVSETINDEDISLLPESEEQEREEDGSEIEADDKEDLEDLEEEEVSDMGNDDPEMGERAENSSKSDLRKSPVFSDEDSDLDFDISKLEQQSKVQNKGQGKPREKSIVDDKFFKLSEMEAYLENIEKEEERKDDNDEEEEDIDFFEDIDSDEDEGGLFGSKKLKSGKSSRNLKYKDFFDPVESDEDITNVHDDELDSNKEDDEIAEEEAEELSISETDEDDDLQENEDNKQHKESLKRVTFALPDDAETEDTGVLNVKKNSDEVKSSFEKRQEKMNEKIASLEKELLEKKPWQLQGEVTAQKRPENSLLEETLHFDHAVRMAPVITEETTLQLEDIIKQRIRDQAWDDVVRKEKPKEDAYEYKKRLTLDHEKSKLSLAEIYEQEYIKLNQQKTAEEENPEHVEIQKMMDSLFLKLDALSNFHFIPKPPVPEIKVVSNLPAITMEEVAPVSVSDAALLAPEEIKEKNKAGDIKTAAEKTATDKKRERRKKKYQKRMKIKEKEKRRKLLEKSSVDQAGKYSKTVASEKLKQLTKTGKASFIKDEGKDKALKSSQAFFSKLQDQVKMQINDAKKTEKKKKKRQDISVHKLKL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
80
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00566 As Substrate

Site PTM Type Enzyme
T10 Phosphorylation
T22 Phosphorylation
S37 Phosphorylation
K38 Ubiquitination
K51 Ubiquitination
S61 Phosphorylation
S120 Phosphorylation
S139 Phosphorylation
S158 Phosphorylation
S163 Phosphorylation
S167 Phosphorylation
S171 Phosphorylation
S178 Phosphorylation
K185 Sumoylation
K189 Acetylation
K193 Acetylation
K197 Acetylation
S198 Phosphorylation
K203 Ubiquitination
S208 Phosphorylation
S242 Phosphorylation
S275 Phosphorylation
T280 Phosphorylation
S289 Phosphorylation
S305 Phosphorylation
S307 Phosphorylation
T309 Phosphorylation
T332 Phosphorylation Q96GD4 (AURKB)
T341 Phosphorylation
S373 Phosphorylation
K376 Ubiquitination
K382 Sumoylation
S399 Phosphorylation
T418 Phosphorylation
Y452 Phosphorylation
Y454 Phosphorylation
T459 Phosphorylation
K466 Ubiquitination
Y477 Phosphorylation
K479 Ubiquitination
K484 Ubiquitination
K498 Ubiquitination
S502 Phosphorylation
K518 Ubiquitination
K559 Acetylation
K564 Acetylation
K574 Acetylation
K609 Acetylation
S629 Phosphorylation
K632 Sumoylation
K641 Methylation

Research Backgrounds

Function:

Component of the 60-80S U3 small nucleolar ribonucleoprotein (U3 snoRNP). Required for the early cleavages during pre-18S ribosomal RNA processing.

PTMs:

Phosphorylated in M (mitotic) phase.

Subcellular Location:

Nucleus>Nucleolus. Chromosome.
Note: Fibrillar region of the nucleolus (PubMed:9450966). After dissolution of the nucleolus in early M phase becomes associated with chromosomes through metaphase and anaphase (PubMed:9450966). In telophase localized to small cellular prenucleolar bodies that not always contain fibrillarin (PubMed:9450966). The reassociation with nucleolus is preceeded by the arrival of fibrillarin (PubMed:9450966).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of a heterotrimeric complex containing IMP3, IMP4 and MPHOSPH10. Interacts with IMP3 and IMP4.

Family&Domains:

Belongs to the MPP10 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

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