Phospho-GFAP (Ser38) Antibody - #AF3166
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF3166, RRID:AB_2834598.
Fold/Unfold
wu:fb34h11; ALXDRD; cb345; etID36982.3; FLJ42474; FLJ45472; GFAP; GFAP_HUMAN; gfapl; Glial fibrillary acidic protein; Intermediate filament protein; wu:fk42c12; xx:af506734; zgc:110485;
Immunogens
- P14136 GFAP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MERRRITSAARRSYVSSGEMMVGGLAPGRRLGPGTRLSLARMPPPLPTRVDFSLAGALNAGFKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNLAQDLATVRQKLQDETNLRLEAENNLAAYRQEADEATLARLDLERKIESLEEEIRFLRKIHEEEVRELQEQLARQQVHVELDVAKPDLTAALKEIRTQYEAMASSNMHEAEEWYRSKFADLTDAAARNAELLRQAKHEANDYRRQLQSLTCDLESLRGTNESLERQMREQEERHVREAASYQEALARLEEEGQSLKDEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P14136 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T7 | Phosphorylation | Q96GD4 (AURKB) , P17612 (PRKACA) , Q13464 (ROCK1) | Uniprot |
| S8 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| R11 | Methylation | Uniprot | |
| R12 | Methylation | Uniprot | |
| S13 | Phosphorylation | Q13464 (ROCK1) , P17612 (PRKACA) , Q96GD4 (AURKB) | Uniprot |
| Y14 | Phosphorylation | Uniprot | |
| S17 | Phosphorylation | Uniprot | |
| S38 | Phosphorylation | Q96GD4 (AURKB) , Q13464 (ROCK1) , P17612 (PRKACA) | Uniprot |
| K95 | Acetylation | Uniprot | |
| Y116 | Phosphorylation | Uniprot | |
| T131 | Phosphorylation | Uniprot | |
| T150 | Phosphorylation | Uniprot | |
| K154 | Acetylation | Uniprot | |
| Y172 | Phosphorylation | Uniprot | |
| K189 | Acetylation | Uniprot | |
| K228 | Acetylation | Uniprot | |
| T240 | Phosphorylation | Uniprot | |
| Y242 | Phosphorylation | Uniprot | |
| K260 | Acetylation | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| Y324 | Phosphorylation | Uniprot | |
| K339 | Acetylation | Uniprot | |
| Y349 | Phosphorylation | Uniprot |
Research Backgrounds
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Phosphorylated by PKN1.
Cytoplasm.
Note: Associated with intermediate filaments.
Expressed in cells lacking fibronectin.
Interacts with SYNM (By similarity). Isoform 3 interacts with PSEN1 (via N-terminus).
Belongs to the intermediate filament family.
Research Fields
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
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