Product: STX6 Antibody
Catalog: DF9956
Description: Rabbit polyclonal antibody to STX6
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 29 kDa; 29kD(Calculated).
Uniprot: O43752
RRID: AB_2843150

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
STX6 Antibody detects endogenous levels of total STX6.
RRID:
AB_2843150
Cite Format: Affinity Biosciences Cat# DF9956, RRID:AB_2843150.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

STX 6; STX6; STX-6; STX6_HUMAN; Syntaxin-6; Syntaxin6;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MSMEDPFFVVKGEVQKAVNTAQGLFQRWTELLQDPSTATREEIDWTTNELRNNLRSIEWDLEDLDETISIVEANPRKFNLDATELSIRKAFITSTRQVVRDMKDQMSTSSVQALAERKNRQALLGDSGSQNWSTGTTDKYGRLDRELQRANSHFIEEQQAQQQLIVEQQDEQLELVSGSIGVLKNMSQRIGGELEEQAVMLEDFSHELESTQSRLDNVMKKLAKVSHMTSDRRQWCAIAILFAVLLVVLILFLVL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
83
Zebrafish
83
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43752 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
K77 Ubiquitination
T83 Phosphorylation
S86 Phosphorylation
K89 Ubiquitination
S107 Phosphorylation
K118 Ubiquitination
S127 Phosphorylation
S129 Phosphorylation
T134 Phosphorylation
T137 Phosphorylation
K139 Ubiquitination
Y140 Phosphorylation
S152 Phosphorylation
K184 Ubiquitination
T229 Phosphorylation
S230 Phosphorylation

Research Backgrounds

Function:

Involved in intracellular vesicle trafficking.

Subcellular Location:

Golgi apparatus membrane>Single-pass type IV membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Identified in a complex containing STX6, STX12, VAMP4 and VTI1A. Binds EEA1 (By similarity). Interacts with VPS45A (By similarity). Interacts with MARCHF2 and MARCHF3 (By similarity). Interacts with GOPC. Interacts with UHRF1BP1L (via C-terminal coiled-coil domain). Interacts with BAIAP3; this interaction is increased in the presence of calcium.

Family&Domains:

Belongs to the syntaxin family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > SNARE interactions in vesicular transport.

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For Research Use Only.
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