SYT4 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis SYT4 using HeLa whole cell lysates.

DF9954 at 1/100 staining Rat brain tissue by IHC-P.

Product:	SYT4 Antibody
Catalog:	DF9954
Description:	Rabbit polyclonal antibody to SYT4
Application:	WB IHC IF/ICC
Reactivity:	Human
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	48 kDa; 48kD(Calculated).
Uniprot:	Q9H2B2
RRID:	AB_2843148

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

SYT4 Antibody detects endogenous levels of total SYT4.

RRID:

AB_2843148
Cite Format: Affinity Biosciences Cat# DF9954, RRID:AB_2843148.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

HsT1192; KIAA1342; Synaptotagmin IV; Synaptotagmin4; SynaptotagminIV; SYT 4; Syt IV; SYT4; SytIV;

Immunogens

Immunogen:

Uniprot:

Q9H2B2(SYT4_HUMAN) >>Visit HPA database.

Gene(ID):

SYT4(6860)

Expression:

Q9H2B2 SYT4_HUMAN:

Expressed in melanocytes (PubMed:23999003). Expressed in brain. Within brain, expression is highest in hippocampus, with substantial levels also detected in amygdala and thalamus (PubMed:23999003).

Sequence:

Q9H2B2 SYT4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAPITTSREEFDEIPTVVGIFSAFGLVFTVSLFAWICCQRKSSKSNKTPPYKFVHVLKGVDIYPENLNSKKKFGADDKNEVKNKPAVPKNSLHLDLEKRDLNGNFPKTNLKPGSPSDLENATPKLFLEGEKESVSPESLKSSTSLTSEEKQEKLGTLFFSLEYNFERKAFVVNIKEARGLPAMDEQSMTSDPYIKMTILPEKKHKVKTRVLRKTLDPAFDETFTFYGIPYTQIQELALHFTILSFDRFSRDDIIGEVLIPLSGIELSEGKMLMNREIIKRNVRKSSGRGELLISLCYQSTTNTLTVVVLKARHLPKSDVSGLSDPYVKVNLYHAKKRISKKKTHVKKCTPNAVFNELFVFDIPCEGLEDISVEFLVLDSERGSRNEVIGQLVLGAAAEGTGGEHWKEICDYPRRQIAKWHVLCDG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Chicken

100

Rabbit

100

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H2B2 As Substrate

Q9H2B2

Site	PTM Type	Enzyme	Source
K52	Ubiquitination		Uniprot
K58	Ubiquitination		Uniprot
Y63	Phosphorylation		Uniprot
K70	Ubiquitination		Uniprot
K78	Ubiquitination		Uniprot
T108	Phosphorylation		Uniprot
K111	Ubiquitination		Uniprot
S114	Phosphorylation		Uniprot
S135	Phosphorylation	P45983 (MAPK8)	Uniprot

Research Backgrounds

Q9H2B2_SYT4_HUMAN

Function:

Synaptotagmin family member which does not bind Ca(2+) (By similarity). Involved in neuronal dense core vesicles (DCVs) mobility through its interaction with KIF1A. Upon increased neuronal activity, phosphorylation by MAPK8/JNK1 destabilizes the interaction with KIF1A and captures DCVs to synapses (By similarity). Plays a role in dendrite formation by melanocytes.

PTMs:

Phosphorylation at Ser-135 by MAPK8/JNK1 reduces interaction with KIF1A and neuronal dense core vesicles mobility.

Subcellular Location:

Cytoplasmic vesicle>Secretory vesicle>Neuronal dense core vesicle membrane>Single-pass membrane protein.

Tissue Specificity:

Expressed in melanocytes. Expressed in brain. Within brain, expression is highest in hippocampus, with substantial levels also detected in amygdala and thalamus.

Subunit Structure:

Interacts with KIF1A; the interaction increases in presence of calcium and decreases when SYT4 is phosphorylated at Ser-135.

Family&Domains:

Unlike in other synaptotagmin family members, the first C2 domain/C2A does not bind Ca(2+) neither mediates Ca(2+)-dependent phospholipid binding. An aspartate-to-serine substitution in this domain inactivates Ca(2+)/phospho-lipid binding.

Belongs to the synaptotagmin family.

Restrictive clause

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SYT4 Antibody - #DF9954