SLC9A1 Antibody - #DF9933

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Product: SLC9A1 Antibody
Catalog: DF9933
Description: Rabbit polyclonal antibody to SLC9A1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 91 kDa; 91kD(Calculated).
Uniprot: P19634
RRID: AB_2843127

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
SLC9A1 Antibody detects endogenous levels of total SLC9A1.
RRID:
AB_2843127
Cite Format: Affinity Biosciences Cat# DF9933, RRID:AB_2843127.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

amiloride-sensitive; APNH; APNH1; FLJ42224; Na Li countertransporter; Na(+)/H(+) antiporter; Na(+)/H(+) exchanger 1; Na+ H+ antiporter amiloride-sensitive; Na+ H+ antiporter; Na+ H+ exchanger 1; NHE-1; NHE1; OTTHUMP00000004468; SL9A1_HUMAN; SLC9A1; Sodium hydrogen exchanger 1; Sodium/hydrogen exchanger 1; solute carrier family 9; Solute carrier family 9 member 1; Solute carrier family 9 sodium hydrogen exchanger isoform 1 antiporter Na+ H+ amiloride sensitive; Solute carrier family 9 subfamily A (NHE1 cation proton antiporter 1) member 1; Solute carrier family 9 subfamily A member 1;

Immunogens

Immunogen:
Uniprot:

P19634(SL9A1_HUMAN) >>Visit HPA database.

Gene(ID):
SLC9A1(6548)
Expression:
P19634 SL9A1_HUMAN:

Kidney and intestine.

Sequence:
MVLRSGICGLSPHRIFPSLLVVVALVGLLPVLRSHGLQLSPTASTIRSSEPPRERSIGDVTTAPPEVTPESRPVNHSVTDHGMKPRKAFPVLGIDYTHVRTPFEISLWILLACLMKIGFHVIPTISSIVPESCLLIVVGLLVGGLIKGVGETPPFLQSDVFFLFLLPPIILDAGYFLPLRQFTENLGTILIFAVVGTLWNAFFLGGLMYAVCLVGGEQINNIGLLDNLLFGSIISAVDPVAVLAVFEEIHINELLHILVFGESLLNDAVTVVLYHLFEEFANYEHVGIVDIFLGFLSFFVVALGGVLVGVVYGVIAAFTSRFTSHIRVIEPLFVFLYSYMAYLSAELFHLSGIMALIASGVVMRPYVEANISHKSHTTIKYFLKMWSSVSETLIFIFLGVSTVAGSHHWNWTFVISTLLFCLIARVLGVLGLTWFINKFRIVKLTPKDQFIIAYGGLRGAIAFSLGYLLDKKHFPMCDLFLTAIITVIFFTVFVQGMTIRPLVDLLAVKKKQETKRSINEEIHTQFLDHLLTGIEDICGHYGHHHWKDKLNRFNKKYVKKCLIAGERSKEPQLIAFYHKMEMKQAIELVESGGMGKIPSAVSTVSMQNIHPKSLPSERILPALSKDKEEEIRKILRNNLQKTRQRLRSYNRHTLVADPYEEAWNQMLLRRQKARQLEQKINNYLTVPAHKLDSPTMSRARIGSDPLAYEPKEDLPVITIDPASPQSPESVDLVNEELKGKVLGLSRDPAKVAEEDEDDDGGIMMRSKETSSPGTDDVFTPAPSDSPSSQRIQRCLSDPGPHPEPGEGEPFFPKGQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P19634 As Substrate

Site PTM Type Enzyme
S5 Phosphorylation
S11 Phosphorylation
S18 Phosphorylation
T42 Phosphorylation
N75 N-Glycosylation
Y96 Phosphorylation
S158 Phosphorylation
S372 Phosphorylation
K559 Ubiquitination
K560 Ubiquitination
Y577 Phosphorylation
K583 Ubiquitination
S599 Phosphorylation
S602 Phosphorylation
T603 Phosphorylation
S605 Phosphorylation
K612 Ubiquitination
S616 Phosphorylation
S624 Phosphorylation
K625 Ubiquitination
S648 Phosphorylation P31749 (AKT1)
T653 Phosphorylation O75116 (ROCK2) , Q13464 (ROCK1)
Y659 Phosphorylation
Y683 Phosphorylation
T685 Phosphorylation
S693 Phosphorylation P28482 (MAPK1)
T695 Phosphorylation
S697 Phosphorylation
S703 Phosphorylation P31749 (AKT1) , Q15418 (RPS6KA1)
Y708 Phosphorylation
T718 Phosphorylation Q16539 (MAPK14)
S723 Phosphorylation Q16539 (MAPK14)
S726 Phosphorylation Q16539 (MAPK14)
S729 Phosphorylation Q16539 (MAPK14)
K738 Ubiquitination
K750 Ubiquitination
S766 Phosphorylation
S770 Phosphorylation P28482 (MAPK1)
S771 Phosphorylation
T774 Phosphorylation
T779 Phosphorylation
S783 Phosphorylation
S785 Phosphorylation P28482 (MAPK1)
S787 Phosphorylation
S788 Phosphorylation
S796 Phosphorylation P31749 (AKT1)

Research Backgrounds

Function:

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.

PTMs:

O-glycosylated.

Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is reduced by CHP1 (By similarity).

Subcellular Location:

Membrane>Multi-pass membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein.
Note: Colocalizes with CHP1 at the reticulum endoplasmic (By similarity). Colocalizes with CHP1 and CHP2 at the plasma membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Kidney and intestine.

Subunit Structure:

Oligomer (By similarity). Interacts with CALM1 in a calcium-dependent manner. Interacts with TESC. Interacts (via the juxtamembrane region of the cytoplasmic C-terminal domain) with CHP1; the interaction occurs at the plasma membrane in a calcium-dependent manner. Interacts with CHP2; the interaction occurs in a calcium-dependent manner.

Family&Domains:

Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Circulatory system > Cardiac muscle contraction.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

· Organismal Systems > Digestive system > Salivary secretion.

· Organismal Systems > Digestive system > Gastric acid secretion.

· Organismal Systems > Digestive system > Pancreatic secretion.

References

1). Cardioprotective role of A-cycloglycosylated derivative of Rubiadin in diabetic cardiomyopathy in rats. International Immunopharmacology, 2023 (PubMed: 36989899) [IF=5.6]
2). Potential Therapeutic Effect of Citronellal on Diabetic Cardiomyopathy in Experimental Rats. Evidence-based Complementary and Alternative Medicine, 2021 (PubMed: 34840590)

Application: IHC    Species: Rat    Sample: myocardial tissues

Figure 4 CT repressing the apoptosis of DCM rat cardiomyocytes by influencing the expression of NHE1 protein. (a) Representative immunohistochemical staining of NHE1 expression in each group. (b) Representative immunofluorescence staining of Bcl-2 and Bax expression, respectively. (c) Western blot analysis of NHE1, Bcl-2, and Bax expression, respectively. (d) The quantitative analysis of the expression of NHE1, Bcl-2, and Bax, respectively. n = 6 per group. Data are mean ± SD; ∗p < 0.05 vs. the control group; #p < 0.05 vs. the DCM group; ns, not significant.

Application: WB    Species: Rat    Sample: myocardial tissues

Figure 4 CT repressing the apoptosis of DCM rat cardiomyocytes by influencing the expression of NHE1 protein. (a) Representative immunohistochemical staining of NHE1 expression in each group. (b) Representative immunofluorescence staining of Bcl-2 and Bax expression, respectively. (c) Western blot analysis of NHE1, Bcl-2, and Bax expression, respectively. (d) The quantitative analysis of the expression of NHE1, Bcl-2, and Bax, respectively. n = 6 per group. Data are mean ± SD; ∗p < 0.05 vs. the control group; #p < 0.05 vs. the DCM group; ns, not significant.

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