Product: SLC9A1 Antibody
Catalog: DF9933
Description: Rabbit polyclonal antibody to SLC9A1
Application: WB IHC IF/ICC
Cited expt.: WB, IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 91 kDa; 91kD(Calculated).
Uniprot: P19634
RRID: AB_2843127

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
SLC9A1 Antibody detects endogenous levels of total SLC9A1.
RRID:
AB_2843127
Cite Format: Affinity Biosciences Cat# DF9933, RRID:AB_2843127.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

amiloride-sensitive; APNH; APNH1; FLJ42224; Na Li countertransporter; Na(+)/H(+) antiporter; Na(+)/H(+) exchanger 1; Na+ H+ antiporter amiloride-sensitive; Na+ H+ antiporter; Na+ H+ exchanger 1; NHE-1; NHE1; OTTHUMP00000004468; SL9A1_HUMAN; SLC9A1; Sodium hydrogen exchanger 1; Sodium/hydrogen exchanger 1; solute carrier family 9; Solute carrier family 9 member 1; Solute carrier family 9 sodium hydrogen exchanger isoform 1 antiporter Na+ H+ amiloride sensitive; Solute carrier family 9 subfamily A (NHE1 cation proton antiporter 1) member 1; Solute carrier family 9 subfamily A member 1;

Immunogens

Immunogen:

A synthesized peptide derived from human SLC9A1, corresponding to a region within the internal amino acids.

Uniprot:
Gene(ID):
Expression:
P19634 SL9A1_HUMAN:

Kidney and intestine.

Sequence:
MVLRSGICGLSPHRIFPSLLVVVALVGLLPVLRSHGLQLSPTASTIRSSEPPRERSIGDVTTAPPEVTPESRPVNHSVTDHGMKPRKAFPVLGIDYTHVRTPFEISLWILLACLMKIGFHVIPTISSIVPESCLLIVVGLLVGGLIKGVGETPPFLQSDVFFLFLLPPIILDAGYFLPLRQFTENLGTILIFAVVGTLWNAFFLGGLMYAVCLVGGEQINNIGLLDNLLFGSIISAVDPVAVLAVFEEIHINELLHILVFGESLLNDAVTVVLYHLFEEFANYEHVGIVDIFLGFLSFFVVALGGVLVGVVYGVIAAFTSRFTSHIRVIEPLFVFLYSYMAYLSAELFHLSGIMALIASGVVMRPYVEANISHKSHTTIKYFLKMWSSVSETLIFIFLGVSTVAGSHHWNWTFVISTLLFCLIARVLGVLGLTWFINKFRIVKLTPKDQFIIAYGGLRGAIAFSLGYLLDKKHFPMCDLFLTAIITVIFFTVFVQGMTIRPLVDLLAVKKKQETKRSINEEIHTQFLDHLLTGIEDICGHYGHHHWKDKLNRFNKKYVKKCLIAGERSKEPQLIAFYHKMEMKQAIELVESGGMGKIPSAVSTVSMQNIHPKSLPSERILPALSKDKEEEIRKILRNNLQKTRQRLRSYNRHTLVADPYEEAWNQMLLRRQKARQLEQKINNYLTVPAHKLDSPTMSRARIGSDPLAYEPKEDLPVITIDPASPQSPESVDLVNEELKGKVLGLSRDPAKVAEEDEDDDGGIMMRSKETSSPGTDDVFTPAPSDSPSSQRIQRCLSDPGPHPEPGEGEPFFPKGQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.

PTMs:

O-glycosylated.

Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is reduced by CHP1 (By similarity).

Subcellular Location:

Membrane>Multi-pass membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein.
Note: Colocalizes with CHP1 at the reticulum endoplasmic (By similarity). Colocalizes with CHP1 and CHP2 at the plasma membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Kidney and intestine.

Family&Domains:

Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Circulatory system > Cardiac muscle contraction.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

· Organismal Systems > Digestive system > Salivary secretion.

· Organismal Systems > Digestive system > Gastric acid secretion.

· Organismal Systems > Digestive system > Pancreatic secretion.

References

1). Targeting macrophage to myofibroblast transition by circ_0001103 for subretinal fibrosis treatment. Journal of translational medicine, 2025 (PubMed: 40022123) [IF=7.4]

2). Cardioprotective role of A-cycloglycosylated derivative of Rubiadin in diabetic cardiomyopathy in rats. International Immunopharmacology, 2023 (PubMed: 36989899) [IF=4.8]

3). Potential Therapeutic Effect of Citronellal on Diabetic Cardiomyopathy in Experimental Rats. Evidence-based Complementary and Alternative Medicine, 2021 (PubMed: 34840590)

Application: IHC    Species: Rat    Sample: myocardial tissues

Figure 4 CT repressing the apoptosis of DCM rat cardiomyocytes by influencing the expression of NHE1 protein. (a) Representative immunohistochemical staining of NHE1 expression in each group. (b) Representative immunofluorescence staining of Bcl-2 and Bax expression, respectively. (c) Western blot analysis of NHE1, Bcl-2, and Bax expression, respectively. (d) The quantitative analysis of the expression of NHE1, Bcl-2, and Bax, respectively. n = 6 per group. Data are mean ± SD; ∗p < 0.05 vs. the control group; #p < 0.05 vs. the DCM group; ns, not significant.

Application: WB    Species: Rat    Sample: myocardial tissues

Figure 4 CT repressing the apoptosis of DCM rat cardiomyocytes by influencing the expression of NHE1 protein. (a) Representative immunohistochemical staining of NHE1 expression in each group. (b) Representative immunofluorescence staining of Bcl-2 and Bax expression, respectively. (c) Western blot analysis of NHE1, Bcl-2, and Bax expression, respectively. (d) The quantitative analysis of the expression of NHE1, Bcl-2, and Bax, respectively. n = 6 per group. Data are mean ± SD; ∗p < 0.05 vs. the control group; #p < 0.05 vs. the DCM group; ns, not significant.

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