Phospho-Vitamin D Receptor (Ser208) Antibody

WB
IF/ICC
pElisa
Cites

1/4

Western blot analysis of Phospho-Vitamin D Receptor (Ser208) Antibody expression in heatshock treated HT29 cells lysates.

AF3159 staining A549 cells cells by ICC/IF.

peptide-ELISA analysis of AF3159.

Figure 2Podocyte-Specific Jaml Deletion Alleviates Renal Injury in Diabetic Mice.

Product:	Phospho-Vitamin D Receptor (Ser208) Antibody
Catalog:	AF3159
Description:	Rabbit polyclonal antibody to Phospho-Vitamin D Receptor (Ser208)
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Pig, Horse, Chicken
Mol.Wt.:	55kDa; 48kD(Calculated).
Uniprot:	P11473
RRID:	AB_2834594

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(86%), Horse(86%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

Phospho-Vitamin D Receptor (Ser208) Antibody detects endogenous levels of Vitamin D Receptor only when phosphorylated at Serine 208.

RRID:

AB_2834594
Cite Format: Affinity Biosciences Cat# AF3159, RRID:AB_2834594.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

1 25 dihydroxyvitamin D3 receptor; 1; 1,25 dihydroxyvitamin D3 receptor; 1,25-@dihydroxyvitamin D3 receptor; 25-dihydroxyvitamin D3 receptor; Member 1; NR1I1; Nuclear receptor subfamily 1 group I member 1; PPP1R163; Protein phosphatase 1, regulatory subunit 163; VDR; VDR_HUMAN; Vitamin D (1,25- dihydroxyvitamin D3) receptor; Vitamin D hormone receptor; Vitamin D nuclear receptor variant 1; Vitamin D receptor; Vitamin D3 receptor;

Immunogens

Immunogen:

Uniprot:

P11473(VDR_HUMAN) >>Visit HPA database.

Gene(ID):

VDR(7421)

Description:

Nuclear hormone receptor. VDR mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes.

Sequence:

P11473 VDR_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEAMAASTSLPDPGDFDRNVPRICGVCGDRATGFHFNAMTCEGCKGFFRRSMKRKALFTCPFNGDCRITKDNRRHCQACRLKRCVDIGMMKEFILTDEEVQRKREMILKRKEEEALKDSLRPKLSEEQQRIIAILLDAHHKTYDPTYSDFCQFRPPVRVNDGGGSHPSRPNSRHTPSFSGDSSSSCSDHCITSSDMMDSSSFSNLDLSEEDSDDPSVTLELSQLSMLPHLADLVSYSIQKVIGFAKMIPGFRDLTSEDQIVLLKSSAIEVIMLRSNESFTMDDMSWTCGNQDYKYRVSDVTKAGHSLELIEPLIKFQVGLKKLNLHEEEHVLLMAICIVSPDRPGVQDAALIEAIQDRLSNTLQTYIRCRHPPPGSHLLYAKMIQKLADLRSLNEEHSKQYRCLSFQPECSMKLTPLVLEVFGNEIS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Chicken

100

Pig

Horse

Bovine

Sheep

Dog

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P11473 As Substrate

P11473

Site	PTM Type	Enzyme	Source
S51	Phosphorylation	P17252 (PRKCA) , P05771 (PRKCB)	Uniprot
K117	Ubiquitination		Uniprot
S119	Phosphorylation		Uniprot
K123	Ubiquitination		Uniprot
S125	Phosphorylation		Uniprot
S172	Phosphorylation		Uniprot
T175	Phosphorylation		Uniprot
S182	Phosphorylation	P17612 (PRKACA)	Uniprot
S208	Phosphorylation	P68400 (CSNK2A1)	Uniprot
Y293	Phosphorylation		Uniprot
K302	Ubiquitination		Uniprot
Y380	Phosphorylation		Uniprot
K386	Ubiquitination		Uniprot
S392	Phosphorylation		Uniprot
K399	Ubiquitination		Uniprot

Research Backgrounds

P11473_VDR_HUMAN

Function:

Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells. Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR. The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes. Plays a central role in calcium homeostasis (By similarity).

Subcellular Location:

Nucleus. Cytoplasm.
Note: Localizes mainly to the nucleus (PubMed:28698609, PubMed:12145331). Localization to the nucleus is enhanced by vitamin D3.

Subunit Structure:

Homodimer in the absence of bound vitamin D3. Heterodimer with RXRA after vitamin D3 binding. Interacts with MED1, NCOA1, NCOA2, NCOA3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with the corepressor NCOR1. Interacts with SNW1. Interacts with IRX4, the interaction does not affect its transactivation activity. Interacts with CRY1 (By similarity). Interacts with CRY2 in a ligand-dependent manner (By similarity).

Family&Domains:

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Belongs to the nuclear hormone receptor family. NR1 subfamily.

Research Fields

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.

· Organismal Systems > Digestive system > Mineral absorption.

References

1). Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism. Cell metabolism (PubMed: 33186558) [IF=29.0]

Application: IF/ICC Species: Mouse Sample:

Figure 2Podocyte-Specific Jaml Deletion Alleviates Renal Injury in Diabetic Mice

2). Phloretin ameliorates diabetic nephropathy by inhibiting nephrin and podocin reduction through a non-hypoglycemic effect. Food & Function (PubMed: 35622066) [IF=6.1]

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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Phospho-Vitamin D Receptor (Ser208) Antibody - #AF3159