MCM2 Antibody | Affinity Biosciences

WB
IHC
IF/ICC
Cites

1/13

Western blot analysis of extracts from various samples, using MCM2 Antibody.

AF0206 at 1/100 staining human colon carcinoma tissue sections by IHC-P.

AF0206 at 1/100 staining Human esophageal cancer by IHC-P.

AF0206 at 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P.

AF0206 at 1/100 staining Human kidney cancer by IHC-P.

AF0206 at 1/100 staining Human lung cancer and adjacent normal tissues by IHC-P.

AF0206 at 1/100 staining Human lung cancer by IHC-P.

AF0206 at 1/100 staining Human liver cancer and adjacent normal tissues by IHC-P.

AF0206 staining Hela cells by IF/ICC.

AF0206 staining A549 cells by IF/ICC.

Figure 3 High expression of miR-301 can promote the invasion, proliferation of breast cancer cells as well as inhibit their apoptosis.

Product:	MCM2 Antibody
Catalog:	AF0206
Description:	Rabbit polyclonal antibody to MCM2
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Horse, Rabbit, Dog
Mol.Wt.:	120kDa; 102kD(Calculated).
Uniprot:	P49736
RRID:	AB_2833393

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(86%), Horse(86%), Rabbit(86%), Dog(86%)

Clonality:

Polyclonal

Specificity:

MCM2 Antibody detects endogenous levels of total MCM2.

RRID:

AB_2833393
Cite Format: Affinity Biosciences Cat# AF0206, RRID:AB_2833393.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BM28; CCNL 1; CCNL1; CDC like 1; cdc19; CDCL 1; CDCL1; Cell devision cycle like 1; Cyclin like 1; D3S3194; DNA replication licensing factor MCM2; KIAA0030; MCM 2; MCM2; MCM2 minichromosome maintenance deficient 2 mitotin; MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae); MCM2 minichromosome maintenance deficient 2, mitotin; MCM2_HUMAN; MGC10606; Minichromosome maintenance complex component 2; Minichromosome maintenance deficient 2 (mitotin); Minichromosome maintenance deficient 2 mitotin; Minichromosome maintenance protein 2; Minichromosome maintenance protein 2 homolog; Mitotin; Nuclear protein BM28; OTTHUMP00000216047; OTTHUMP00000216050;

Immunogens

Immunogen:

Uniprot:

P49736(MCM2_HUMAN) >>Visit HPA database.

Gene(ID):

MCM2(4171)

Description:

MCM2 a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division.

Sequence:

P49736 MCM2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAESSESFTMASSPAQRRRGNDPLTSSPGRSSRRTDALTSSPGRDLPPFEDESEGLLGTEGPLEEEEDGEELIGDGMERDYRAIPELDAYEAEGLALDDEDVEELTASQREAAERAMRQRDREAGRGLGRMRRGLLYDSDEEDEERPARKRRQVERATEDGEEDEEMIESIENLEDLKGHSVREWVSMAGPRLEIHHRFKNFLRTHVDSHGHNVFKERISDMCKENRESLVVNYEDLAAREHVLAYFLPEAPAELLQIFDEAALEVVLAMYPKYDRITNHIHVRISHLPLVEELRSLRQLHLNQLIRTSGVVTSCTGVLPQLSMVKYNCNKCNFVLGPFCQSQNQEVKPGSCPECQSAGPFEVNMEETIYQNYQRIRIQESPGKVAAGRLPRSKDAILLADLVDSCKPGDEIELTGIYHNNYDGSLNTANGFPVFATVILANHVAKKDNKVAVGELTDEDVKMITSLSKDQQIGEKIFASIAPSIYGHEDIKRGLALALFGGEPKNPGGKHKVRGDINVLLCGDPGTAKSQFLKYIEKVSSRAIFTTGQGASAVGLTAYVQRHPVSREWTLEAGALVLADRGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTSLQARCTVIAAANPIGGRYDPSLTFSENVDLTEPIISRFDILCVVRDTVDPVQDEMLARFVVGSHVRHHPSNKEEEGLANGSAAEPAMPNTYGVEPLPQEVLKKYIIYAKERVHPKLNQMDQDKVAKMYSDLRKESMATGSIPITVRHIESMIRMAEAHARIHLRDYVIEDDVNMAIRVMLESFIDTQKFSVMRSMRKTFARYLSFRRDNNELLLFILKQLVAEQVTYQRNRFGAQQDTIEVPEKDLVDKARQINIHNLSAFYDSELFRMNKFSHDLKRKMILQQF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Dog

Rabbit

Bovine

Xenopus

Sheep

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49736 As Substrate

P49736

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
S4	Phosphorylation	P50613 (CDK7) , O00311 (CDC7)	Uniprot
S5	Phosphorylation	P50613 (CDK7) , O00311 (CDC7)	Uniprot
S7	Phosphorylation	P50613 (CDK7) , O00311 (CDC7)	Uniprot
T9	Phosphorylation		Uniprot
S12	Phosphorylation		Uniprot
S13	Phosphorylation	P24941 (CDK2) , P68400 (CSNK2A1) , O00311 (CDC7) , P50613 (CDK7)	Uniprot
T25	Phosphorylation		Uniprot
S26	Phosphorylation		Uniprot
S27	Phosphorylation	P50613 (CDK7) , P24941 (CDK2) , P68400 (CSNK2A1) , O00311 (CDC7)	Uniprot
S31	Phosphorylation	O00311 (CDC7)	Uniprot
S32	Phosphorylation		Uniprot
T35	Phosphorylation		Uniprot
T39	Phosphorylation		Uniprot
S40	Phosphorylation	P68400 (CSNK2A1) , O00311 (CDC7) , P50613 (CDK7) , P24941 (CDK2)	Uniprot
S41	Phosphorylation	O00311 (CDC7) , P24941 (CDK2) , P68400 (CSNK2A1) , P50613 (CDK7)	Uniprot
S53	Phosphorylation	P68400 (CSNK2A1) , P24941 (CDK2) , P50613 (CDK7) , O00311 (CDC7)	Uniprot
T59	Phosphorylation	P50613 (CDK7)	Uniprot
Y81	Phosphorylation		Uniprot
Y90	Phosphorylation		Uniprot
T106	Phosphorylation		Uniprot
S108	Phosphorylation	P50613 (CDK7) , P24941 (CDK2) , P68400 (CSNK2A1) , Q13535 (ATR) , O00311 (CDC7)	Uniprot
R126	Methylation		Uniprot
Y137	Phosphorylation		Uniprot
S139	Phosphorylation	P24941 (CDK2) , P68400 (CSNK2A1) , O00311 (CDC7) , P50613 (CDK7)	Uniprot
T158	Phosphorylation		Uniprot
S170	Phosphorylation		Uniprot
K178	Ubiquitination		Uniprot
K200	Ubiquitination		Uniprot
S209	Phosphorylation		Uniprot
K216	Acetylation		Uniprot
K216	Ubiquitination		Uniprot
S220	Phosphorylation	O00311 (CDC7) , O14965 (AURKA)	Uniprot
K224	Ubiquitination		Uniprot
S229	Phosphorylation		Uniprot
Y234	Phosphorylation		Uniprot
R307	Methylation		Uniprot
C315	S-Nitrosylation		Uniprot
S381	Phosphorylation		Uniprot
K384	Ubiquitination		Uniprot
K394	Ubiquitination		Uniprot
K450	Ubiquitination		Uniprot
T457	Phosphorylation		Uniprot
K462	Ubiquitination		Uniprot
K469	Acetylation		Uniprot
K469	Ubiquitination		Uniprot
K476	Ubiquitination		Uniprot
S484	Phosphorylation		Uniprot
K492	Ubiquitination		Uniprot
T527	Phosphorylation		Uniprot
K529	Ubiquitination		Uniprot
K538	Ubiquitination		Uniprot
S566	Phosphorylation		Uniprot
K591	Ubiquitination		Uniprot
K613	Ubiquitination		Uniprot
R623	Methylation		Uniprot
R677	Methylation		Uniprot
K722	Ubiquitination		Uniprot
K728	Ubiquitination		Uniprot
K734	Ubiquitination		Uniprot
K742	Acetylation		Uniprot
K742	Ubiquitination		Uniprot
K745	Ubiquitination		Uniprot
R751	Methylation		Uniprot
K752	Ubiquitination		Uniprot
S754	Phosphorylation		Uniprot
T757	Phosphorylation		Uniprot
T763	Phosphorylation		Uniprot
S769	Phosphorylation		Uniprot
S801	Phosphorylation		Uniprot
T857	Phosphorylation		Uniprot
K863	Ubiquitination		Uniprot
K868	Ubiquitination		Uniprot
S878	Phosphorylation		Uniprot
K896	Acetylation		Uniprot
K896	Ubiquitination		Uniprot

Research Backgrounds

P49736_MCM2_HUMAN

Function:

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis.

PTMs:

Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.

Subcellular Location:

Nucleus.

Subunit Structure:

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (Probable). Interacts with DBF4 (By similarity). Interacts with KAT7. May interact with MCM10. Component of the replisome complex composed of at least DONSON, MCM2, MCM7, PCNA and TICRR.

Family&Domains:

Belongs to the MCM family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)

· Genetic Information Processing > Replication and repair > DNA replication.

References

1). MiR-301 regulates the SIRT1/SOX2 pathway via CPEB1 in the breast cancer progression. Molecular Therapy-Oncolytics, 2021 (PubMed: 34377766) [IF=5.7]

Application: WB Species: Human Sample: breast cancer cells

Figure 3 High expression of miR-301 can promote the invasion, proliferation of breast cancer cells as well as inhibit their apoptosis. A: Detection of miR-301 expression level by RT-qPCR; B:Detection of breast cancer cell proliferation by CCK-8; C: Distribution chart of breast cancer cell cycle; D: Detection of migration ability of breast cancer cells by Transwell; E, The relative number of invasive breast cancer cells, as measured by Transwell assay. F: Apoptosis rate of breast cancer cells; G: Detection of mRNA expression level of proliferation-related factor MCM2, migration- and invasion-related factors MMP2, apoptosis-related factors caspase-3 by RT-qPCR; H: Detection of protein expression level of proliferation-related factor MCM2, migration- and invasion-related factors MMP2,apoptosis-related factors caspase-3 by Western blot; The continuous variables were expressed by mean ± standard deviation; * P < 0.05 compared with NC group; Paired Sample t-test,Independent Sample t-test or one-way ANOVA were selected to test the inter-group differences of continuous variables; The difference of data of each group at different time was analyzed by Two-way ANOVA. We repeated the experiment three times in order to obtain accurate and stable results

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MCM2 Antibody - #AF0206