CXCL5 Antibody - #DF9919

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Product: CXCL5 Antibody
Catalog: DF9919
Description: Rabbit polyclonal antibody to CXCL5
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Rabbit
Mol.Wt.: 12 kDa; 12kD(Calculated).
Uniprot: P42830
RRID: AB_2843113

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Rabbit(83%)
Clonality:
Polyclonal
Specificity:
CXCL5 Antibody detects endogenous levels of total CXCL5.
RRID:
AB_2843113
Cite Format: Affinity Biosciences Cat# DF9919, RRID:AB_2843113.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AMCFII; C-X-C motif chemokine 5; C-X-C motif chemokine ligand 5; chemokine (C-X-C motif) ligand 5; Cxcl5; CXCL5_HUMAN; ENA 78; ENA-78 (8-78); ENA-78(1-78); ENA-78(9-78); ENA78; Epithelial derived neutrophil activating protein 78; Epithelial-derived neutrophil-activating protein 78; Lipopolysaccharide-induced CXC chemokine; Neutrophil activating peptide ENA 78; Neutrophil-activating peptide ENA-78; neutrophil-activating protein 78; SCYB5; Small inducible cytokine B5; small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78); small inducible cytokine subfamily B, member 5; Small-inducible cytokine B5;

Immunogens

Immunogen:
Uniprot:

P42830(CXCL5_HUMAN) >>Visit HPA database.

Gene(ID):
CXCL5(6374)
Sequence:
MSLLSSRAARVPGPSSSLCALLVLLLLLTQPGPIASAGPAAAVLRELRCVCLQTTQGVHPKMISNLQVFAIGPQCSKVEVVASLKNGKEICLDPEAPFLKKVIQKILDGGNKEN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
83
Pig
0
Horse
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P42830 As Substrate

Site PTM Type Enzyme
S5 Phosphorylation

Research Backgrounds

Function:

Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.

PTMs:

N-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Monomer. Homodimer.

Family&Domains:

Belongs to the intercrine alpha (chemokine CxC) family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

References

1). CXCL5 Downregulation in Villous Tissue Is Correlated With Recurrent Spontaneous Abortion. Frontiers in Immunology, 2021 (PubMed: 34603292) [IF=7.3]

Application: IHC    Species: Human    Sample: placental villous tissue

Figure 1 CXCL5 expression in human placental villous tissue from the RSA group and the control group. (A) Expression of CXCL5 mRNA in the RSA group (n = 15) and control group (n = 13) by RT-PCR. (B, C) Representative immunohistochemical staining images and quantification of CXCL5 in the RSA group (n = 15) and normal group (n = 13). Relative gene expression was normalized to GAPDH. ImageJ is used to quantify the staining intensity. Magnification, ×200 and ×400. Scale bars = 100 μm. Data are presented as the mean ± SD. Results are reported as fold change compared with the control group. (*p < 0.05, **p < 0.01). RSA, recurrent spontaneous abortion; mRNA, messenger RNA; RT‐PCR, quantitative real-time polymerase chain reaction; CXCL5, C-X-C motif chemokine ligand 5; IHC, immunohistochemistry.

Application: IHC    Species: Human    Sample: placental villous tissue

Figure 1 CXCL5 expression in human placental villous tissue from the RSA group and the control group. (A) Expression of CXCL5 mRNA in the RSA group (n = 15) and control group (n = 13) by RT-PCR. (B, C) Representative immunohistochemical staining images and quantification of CXCL5 in the RSA group (n = 15) and normal group (n = 13). Relative gene expression was normalized to GAPDH. ImageJ is used to quantify the staining intensity. Magnification, ×200 and ×400. Scale bars = 100 μm. Data are presented as the mean ± SD. Results are reported as fold change compared with the control group. (*p < 0.05, **p < 0.01). RSA, recurrent spontaneous abortion; mRNA, messenger RNA; RT‐PCR, quantitative real-time polymerase chain reaction; CXCL5, C-X-C motif chemokine ligand 5; IHC, immunohistochemistry.
2). Transcriptomics Reveals Effect of Pulsatilla Decoction Butanol Extract in Alleviating Vulvovaginal Candidiasis by Inhibiting Neutrophil Chemotaxis and Activation via TLR4 Signaling. Pharmaceuticals (Basel, Switzerland), 2024 (PubMed: 38794163) [IF=4.6]
3). Upregulation of ITGB6 in primary palmar hyperhidrosis. Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 2023 (PubMed: 37212774) [IF=2.1]

Application: WB    Species: Human    Sample:

Fig. 6. Regulatory effects of integrin β6 (ITGB6) on CXCL3, CXCL5, CXCL10, CXCL11, and WNT2 expression levels in sweat gland cells. Sweat gland cells extracted from patients with primary palmar hyperhidrosis were treated with blank control or transfected with overexpression negative control (OE NC) or ITGB6 OE. The CXCL3, CXCL5, CXCL10, CXCL11, and WNT2 (A) mRNA (ITGB6 OE compared to control, Tukey’s honest significant difference (HSD), p < 0.001, all), and (B,C) protein expression (ITGB6 OE compared to control, CXCL3, CXCL5, and CXCL11: Tukey’s HSD, all p < 0.001; CXCL10: Tukey’s HSD, p = 0.004; WNT2: Tukey’s HSD, p = 0.027). The data are presented as mean ± 95% confidence interval (95% CI)

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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