Product: CXCL13 Antibody
Catalog: DF9918
Description: Rabbit polyclonal antibody to CXCL13
Application: WB
Reactivity: Human, Monkey
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 13 kDa; 13kD(Calculated).
Uniprot: O43927
RRID: AB_2843112

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Monkey
Prediction:
Pig(86%), Bovine(86%), Sheep(86%), Rabbit(86%), Dog(86%)
Clonality:
Polyclonal
Specificity:
CXCL13 Antibody detects endogenous levels of total CXCL13.
RRID:
AB_2843112
Cite Format: Affinity Biosciences Cat# DF9918, RRID:AB_2843112.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ANGIE; ANGIE2; B cell attracting chemokine 1; B cell-attracting chemokine 1; B lymphocyte chemoattractant; B-cell chemoattractant; B-cell-attracting chemokine 1; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); BCA-1; BLC; BLR1L; C-X-C motif chemokine 13; Chemokine (C-X-C motif) ligand 13; Chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); Chemokine, CXC motif, ligand 13; CXC chemokine BLC; CXCL13; CXL13_HUMAN; SCYB13; Small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant); Small inducible cytokine B13; Small inducible cytokine subfamily B, member 13; Small-inducible cytokine B13;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O43927 CXL13_HUMAN:

Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen.

Sequence:
MKFISTSLLLMLLVSSLSPVQGVLEVYYTSLRCRCVQESSVFIPRRFIDRIQILPRGNGCPRKEIIVWKKNKSIVCVDPQAEWIQRMMEVLRKRSSSTLPVPVFKRKIP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
86
Bovine
86
Sheep
86
Dog
86
Rabbit
86
Xenopus
57
Horse
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43927 As Substrate

Site PTM Type Enzyme
S39 Phosphorylation
S40 Phosphorylation
K63 Sumoylation
K69 Ubiquitination

Research Backgrounds

Function:

Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen.

Family&Domains:

Belongs to the intercrine alpha (chemokine CxC) family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

References

1). Long Noncoding RNA SNHG5 Knockdown Alleviates Neuropathic Pain by Targeting the miR-154-5p/CXCL13 Axis. NEUROCHEMICAL RESEARCH, 2020 (PubMed: 32248399) [IF=4.4]

Application: WB    Species: mouse    Sample:

Fig. 5 |CXCL13 is a down-stream target of miR-154-5p. a The binding site of miR-154-5p on CXCL13 was shown. b The interaction between miR-154-5p and CXCL13 was verified using luciferase activity. c The CXCL13 protein level was detected at day 3, day 7 and day 14 after SNL model establishment.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.