CXCL11 Antibody - #DF9917

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Product: CXCL11 Antibody
Catalog: DF9917
Description: Rabbit polyclonal antibody to CXCL11
Application: WB IHC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.: 10 kDa; 10kD(Calculated).
Uniprot: O14625
RRID: AB_2843111

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(91%), Bovine(91%), Horse(100%), Sheep(100%), Rabbit(82%)
Clonality:
Polyclonal
Specificity:
CXCL11 Antibody detects endogenous levels of total CXCL11.
RRID:
AB_2843111
Cite Format: Affinity Biosciences Cat# DF9917, RRID:AB_2843111.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

b R1; b-R1; Beta-R1; betaR1; C-X-C motif chemokine 11; Chemokine (C-X-C motif) ligand 11; Chemokine C-X-C motif ligand 11; CXC11; CXCL11; CXL11_HUMAN; H174; I TAC; I-TAC; Interferon gamma inducible protein 9; Interferon gamma-inducible protein 9; Interferon inducible T cell alpha chemoattractant; Interferon-inducible T-cell a chemoattractant I-TAC; Interferon-inducible T-cell alpha chemoattractant; IP 9; IP-9; IP9; ITAC; MGC102770; SCYB11; SCYB9B; Small inducible cytokine B11; Small inducible cytokine subfamily B (Cys-X-Cys) member 11; Small inducible cytokine subfamily B (Cys-X-Cys) member 9B; Small inducible cytokine subfamily B, member 11; Small inducible cytokine subfamily B, member 9B; Small-inducible cytokine B11;

Immunogens

Immunogen:
Uniprot:

O14625(CXL11_HUMAN) >>Visit HPA database.

Gene(ID):
CXCL11(6373)
Expression:
O14625 CXL11_HUMAN:

High levels in peripheral blood leukocytes, pancreas and liver astrocytes. Moderate levels in thymus, spleen and lung. Low levels in placenta, prostate and small intestine. Also found in epidermal basal layer keratinocytes in skin disorders.

Sequence:
MSVKGMAIALAVILCATVVQGFPMFKRGRCLCIGPGVKAVKVADIEKASIMYPSNNCDKIEVIITLKENKGQRCLNPKSKQARLIIKKVERKNF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Sheep
100
Pig
91
Bovine
91
Rabbit
82
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14625 As Substrate

Site PTM Type Enzyme
S49 Phosphorylation
Y52 Phosphorylation

Research Backgrounds

Function:

Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

High levels in peripheral blood leukocytes, pancreas and liver astrocytes. Moderate levels in thymus, spleen and lung. Low levels in placenta, prostate and small intestine. Also found in epidermal basal layer keratinocytes in skin disorders.

Family&Domains:

Belongs to the intercrine alpha (chemokine CxC) family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

References

1). Integrated immuno-transcriptomic analysis of ovarian cancer identifies a four-chemokine-dominated subtype with antitumor immune-active phenotype and favorable prognosis. British journal of cancer, 2024 (PubMed: 39095528) [IF=8.8]
2). Study on the relationship between CXCR3 and its ligands and tubal tuberculosis. LIFE SCIENCES, 2021 (PubMed: 33454369) [IF=6.1]

Application: IHC    Species: Human    Sample: tubal tuberculosis

Fig. 4. CXCR3, CXCL9, CXCL10 and CXCL11 IHC staining. A: High-risk pregnancy monitoring control group CXCR3 Immunohistochemistry (×200); B: Mild Tubal Tuberculosis Group CXCR3 Immunohistochemistry (×200); C: Severe Tubal Tuberculosis Group CXCR3 Immunohistochemistry (×200); D: High-risk pregnancy monitoring control group CXCL9 Immunohistochemistry (×200); E: Mild Tubal Tuberculosis Group CXCL9 Immunohistochemistry (×200); F: Severe Tubal Tuberculosis Group CXCL9 Immunohistochemistry (×200); G: High-risk pregnancy monitoring control group CXCL10 Immunohistochemistry (×200); H: Mild Tubal Tuberculosis Group CXCL10 Immunohistochemistry (×200); I: Severe Tubal Tuberculosis Group CXCL10 Immunohistochemistry (×200); J: High-risk pregnancy monitoring control group CXCL11 Immunohistochemistry (×200); K: Mild Tubal Tuberculosis Group CXCL11 Immunohistochemistry (×200); L: Severe Tubal Tuberculosis Group CXCL11 Immunohistochemistry (×200).
3). Upregulation of ITGB6 in primary palmar hyperhidrosis. Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 2023 (PubMed: 37212774) [IF=2.1]

Application: WB    Species: Human    Sample:

Fig. 6. Regulatory effects of integrin β6 (ITGB6) on CXCL3, CXCL5, CXCL10, CXCL11, and WNT2 expression levels in sweat gland cells. Sweat gland cells extracted from patients with primary palmar hyperhidrosis were treated with blank control or transfected with overexpression negative control (OE NC) or ITGB6 OE. The CXCL3, CXCL5, CXCL10, CXCL11, and WNT2 (A) mRNA (ITGB6 OE compared to control, Tukey’s honest significant difference (HSD), p < 0.001, all), and (B,C) protein expression (ITGB6 OE compared to control, CXCL3, CXCL5, and CXCL11: Tukey’s HSD, all p < 0.001; CXCL10: Tukey’s HSD, p = 0.004; WNT2: Tukey’s HSD, p = 0.027). The data are presented as mean ± 95% confidence interval (95% CI)

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