CCL1 Antibody | Affinity Biosciences

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Figure 3 Ferroptosis-inducing and immuno-modulatory properties of ESIONPs@EXO.

Product:	CCL1 Antibody
Catalog:	DF9910
Description:	Rabbit polyclonal antibody to CCL1
Application:	WB IHC
Reactivity:	Human
Mol.Wt.:	11 kDa; 11kD(Calculated).
Uniprot:	P22362
RRID:	AB_2843104

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

CCL1 Antibody detects endogenous levels of total CCL1.

RRID:

AB_2843104
Cite Format: Affinity Biosciences Cat# DF9910, RRID:AB_2843104.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C-C motif chemokine 1; Ccl1; CCL1_HUMAN; Chemokine CC Motif Ligand 1; inflammatory cytokine I-309; P500; SCYA1; SISe; small inducible cytokine A1; Small-inducible cytokine A1; T lymphocyte secreted protein I-309; T lymphocyte secreted protein I-309; T lymphocyte-secreted protein I-309; TCA3;

Immunogens

Immunogen:

Uniprot:

P22362(CCL1_HUMAN) >>Visit HPA database.

Gene(ID):

CCL1(6346)

Sequence:

P22362 CCL1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MQIITTALVCLLLAGMWPEDVDSKSMQVPFSRCCFSFAEQEIPLRAILCYRNTSSICSNEGLIFKLKRGKEACALDTVGWVQRHRKMLRHCPSKRK

Research Backgrounds

P22362_CCL1_HUMAN

Function:

Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8.

Subcellular Location:

Secreted.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the intercrine beta (chemokine CC) family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)

References

1). Iron Oxide Nanoparticles Engineered Macrophage-Derived Exosomes for Targeted Pathological Angiogenesis Therapy. ACS nano, 2024 (PubMed: 38412252) [IF=17.1]

Application: WB Species: Mouse Sample:

Figure 3 Ferroptosis-inducing and immuno-modulatory properties of ESIONPs@EXO. (A) The protein levels of NOX1, CD71, COX2, and GPX4 in ESIONPs@EXO-treated C166 determined by Western blot. (B) Statistical result of the protein levels in ESIONPs@EXO-treated C166. (C) Relative GSH level in ESIONPs@EXO-treated C166. (D) TEM representative images of mitochondria in C166 cells after treatment with ESIONPs@EXO for 24 h. Scale bar: 500 nm (upper) and 200 nm (lower). (E) Contents of various cytokines/chemokines in ESIONPs@EXO determined by protein array analysis. (F) The protein levels of CCL1, TIMP1, TIMP2, TNFα, IL-6, IL-9, CX3CL1, and CCL3 in EXO and ESIONPs@EXO determined by Western blot. (G) Statistical results of the protein levels in ESIONPs@EXO.

2). A novel lymphatic pattern promotes metastasis of cervical cancer in a hypoxic tumour-associated macrophage-dependent manner. ANGIOGENESIS, 2021 (PubMed: 33484377) [IF=9.8]

Application: IF/ICC Species: human Sample: CSCC

Fig. 5 |Sp1high LECs are fundamental to LVEM formation and lymphatic metastasis.i Immunofuorescence staining was applied to analyse Sp1 (purple), CCL1 (red), CD163 (green) and DAPI (blue)expression in CSCC tissues (Scale bar, 50 μm).

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CCL1 Antibody - #DF9910