SLIT3 Antibody | Affinity Biosciences

IHC
IF/ICC
Cites

1/6

DF9909 at 1/100 staining Human pancreatic cancer by IHC-P.

DF9909 at 1/100 staining Rat heart tissue by IHC-P.

DF9909 at 1/100 staining Rat brain tissue by IHC-P.

DF9909 staining Hela cells by IF/ICC.

Figure 2 The identification 4 prognostic EMT-related genes comprising the E-signature.

Figure 1.

Product:	SLIT3 Antibody
Catalog:	DF9909
Description:	Rabbit polyclonal antibody to SLIT3
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	168 kDa; 168kD(Calculated).
Uniprot:	O75094
RRID:	AB_2843103

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%)

Clonality:

Polyclonal

Specificity:

SLIT3 Antibody detects endogenous levels of total SLIT3.

RRID:

AB_2843103
Cite Format: Affinity Biosciences Cat# DF9909, RRID:AB_2843103.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ10764; KIAA0814; MEGF 5; MEGF5; Multiple EGF-like domains protein 5; Multiple epidermal growth factor like domains 5; Multiple epidermal growth factor-like domains protein 5; SLIL 2; SLIL2; SLIT 1; Slit 2; Slit 3; Slit homolog 3 (Drosophila); Slit homolog 3; Slit homolog 3 protein; Slit-3; SLIT1; Slit2; Slit3; SLIT3_HUMAN;

Immunogens

Immunogen:

Uniprot:

O75094(SLIT3_HUMAN) >>Visit HPA database.

Gene(ID):

SLIT3(6586)

Expression:

O75094 SLIT3_HUMAN:

Predominantly expressed in thyroid.

Sequence:

O75094 SLIT3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAPGWAGVGAAVRARLALALALASVLSGPPAVACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTVGQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSEPPSCNANSISCPSPCTCSNNIVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIVKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRSRFSSECFMDLVCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGNPRCQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPRCPEQCTCMETVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAVPRELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKGPVDINIVAKCNACLSSPCKNNGTCTQDPVELYRCACPYSYKGKDCTVPINTCIQNPCQHGGTCHLSDSHKDGFSCSCPLGFEGQRCEINPDDCEDNDCENNATCVDGINNYVCICPPNYTGELCDEVIDHCVPELNLCQHEAKCIPLDKGFSCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDTINGYTCTCPQGFSGPFCEHPPPMVLLQTSPCDQYECQNGAQCIVVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVELASAKVRPQANISLQVATDKDNGILLYKGDNDPLALELYQGHVRLVYDSLSSPPTTVYSVETVNDGQFHSVELVTLNQTLNLVVDKGTPKSLGKLQKQPAVGINSPLYLGGIPTSTGLSALRQGTDRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGCKSCTVCKHGLCRSVEKDSVVCECRPGWTGPLCDQEARDPCLGHRCHHGKCVATGTSYMCKCAEGYGGDLCDNKNDSANACSAFKCHHGQCHISDQGEPYCLCQPGFSGEHCQQENPCLGQVVREVIRRQKGYASCATASKVPIMECRGGCGPQCCQPTRSKRRKYVFQCTDGSSFVEEVERHLECGCLACS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75094 As Substrate

O75094

Site	PTM Type	Source
T417	Phosphorylation	Uniprot
S419	Phosphorylation	Uniprot
K473	Acetylation	Uniprot
K561	Acetylation	Uniprot
S582	Phosphorylation	Uniprot
S605	Phosphorylation	Uniprot
T609	Phosphorylation	Uniprot
S1173	Phosphorylation	Uniprot
T1295	O-Glycosylation	Uniprot
T1385	Phosphorylation	Uniprot
T1387	Phosphorylation	Uniprot
S1388	Phosphorylation	Uniprot
Y1389	Phosphorylation	Uniprot

Research Backgrounds

O75094_SLIT3_HUMAN

Function:

May act as molecular guidance cue in cellular migration, and function may be mediated by interaction with roundabout homolog receptors.

Subcellular Location:

Secreted.

Tissue Specificity:

Predominantly expressed in thyroid.

Research Fields

· Organismal Systems > Development > Axon guidance. (View pathway)

References

1). A novel epithelial-mesenchymal transition (EMT)-related gene signature of predictive value for the survival outcomes in lung adenocarcinoma. Frontiers in Oncology, 2022 (PubMed: 36185284) [IF=4.7]

Application: WB Species: Human Sample: LUAD tumor

Figure 2 The identification 4 prognostic EMT-related genes comprising the E-signature. (A) The standard deviation distribution of all mRNAs. In the left panel, location corresponding to the red columns were standard deviation (SD) of genes with frequency greater than 500, where SD was used as the abscissa and the vertical axis represented the number of genes. The right panel showed that the gene frequency distribution chart obtained by LASSO analysis. The green baseline meant that frequency was equal to 500, the part exceeded the green line was genes with frequency greater than 500. (B) KM curves showed the overall survival (OS) of patients grouped according to expression patterns of 4 prognostic EMT-related genes comprising the E-signature in TCGA-LUAD datasets. (C) Differential expression analysis of DKK1, LOXL2, MGP and SLIT3 originated from TCGA-LUAD dataset. (D) The expression of 4 genes in fresh LUAD tumor samples (T) and adjacent normal-frozen tissues (N) detected by Western blot

2). Targeting Bioinformatics Predicted Biomarkers Associated with Cell Proliferation and Migration for Treating Gliomas: Preclinical Studies in a GL261 Mouse Model. Neuroglia, 2023

Application: IHC Species: Human Sample:

Figure 1. Expressions of PLXNB2, SLIT3, and SPON1 in human high-grade gliomas. Representative immunohistochemistry (IHC) images for PLXNB2 (A), SLIT3 (B), and SPON1 (C) in human GBM tumor sections. (D) Negative control in a low-grade glioma for SLIT3. Magnification is 20× for slides in (A–D). (E) Percentage (%) of patient protein expression (IHC) for PLXNB2, SLIT3, and SPON1 in high-grade (HG) gliomas highly expressed (HG E) or not expressed (HG NE). The % of patient expression for each biomarker in HG was 80% or higher.

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SLIT3 Antibody - #DF9909