SH3GL3 Antibody - #DF9907

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Product: SH3GL3 Antibody
Catalog: DF9907
Description: Rabbit polyclonal antibody to SH3GL3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 39 kDa; 39kD(Calculated).
Uniprot: Q99963
RRID: AB_2843101

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(82%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(100%), Dog(91%)
Clonality:
Polyclonal
Specificity:
SH3GL3 Antibody detects endogenous levels of total SH3GL3.
RRID:
AB_2843101
Cite Format: Affinity Biosciences Cat# DF9907, RRID:AB_2843101.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CNSA3; EEN 2B L3; EEN B2; EEN-B2; EEN2BL3; EENB2; Endophilin 3; Endophilin A3; Endophilin-3; Endophilin-A3; H.sapiens mRNA for protein containing SH3 domain, SH3GL3; HsT19371; SH3 domain containing GRB2 like protein 3; SH3 domain GRB2 like 3; SH3 domain protein 2C; SH3 domain-containing GRB2-like protein 3; SH3D2C; SH3G3_HUMAN; SH3GL3; SH3P13;

Immunogens

Immunogen:
Uniprot:

Q99963(SH3G3_HUMAN) >>Visit HPA database.

Gene(ID):
SH3GL3(6457)
Expression:
Q99963 SH3G3_HUMAN:

Brain and testis.

Sequence:
MSVAGLKKQFHKASQLFSEKISGAEGTKLDDEFLDMERKIDVTNKVVAEILSKTTEYLQPNPAYRAKLGMLNTVSKIRGQVKTTGYPQTEGLLGDCMLKYGKELGEDSTFGNALIEVGESMKLMAEVKDSLDINVKQTFIDPLQLLQDKDLKEIGHHLKKLEGRRLDYDYKKKRVGKIPDEEVRQAVEKFEESKELAERSMFNFLENDVEQVSQLAVFIEAALDYHRQSTEILQELQSKLQMRISAASSVPRREYKPRPVKRSSSELNGVSTTSVVKTTGSNIPMDQPCCRGLYDFEPENQGELGFKEGDIITLTNQIDENWYEGMIHGESGFFPINYVEVIVPLPQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
91
Bovine
91
Sheep
91
Dog
91
Pig
82
Xenopus
64
Chicken
64
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99963 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
S14 Phosphorylation
T83 Phosphorylation
Y86 Phosphorylation
Y168 Phosphorylation
S248 Phosphorylation
Y255 Phosphorylation
K261 Acetylation
S263 Phosphorylation
S264 Phosphorylation
S265 Phosphorylation
K277 Acetylation

Research Backgrounds

Function:

Implicated in endocytosis. May recruit other proteins to membranes with high curvature (By similarity).

Subcellular Location:

Cytoplasm. Early endosome membrane>Peripheral membrane protein.
Note: Associated with postsynaptic endosomes in hippocampal neurons. Associated with presynaptic endosomes in olfactory neurons.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Brain and testis.

Subunit Structure:

Interacts with ARC (By similarity). Interacts with DNM1, SGIP1 and SYNJ1. Interacts with the huntingtin exon 1 protein (HDEX1P) containing a glutamine repeat in the pathological range and promotes formation of insoluble polyglutamine-containing aggregates in vivo. Interacts with DYDC1. Interacts with FASLG. Interacts with ATX2. Interacts with BIN2.

Family&Domains:

An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes.

Belongs to the endophilin family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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