NCKIPSD Antibody - #DF9899
| Product: | NCKIPSD Antibody |
| Catalog: | DF9899 |
| Description: | Rabbit polyclonal antibody to NCKIPSD |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 79 kDa; 79kD(Calculated). |
| Uniprot: | Q9NZQ3 |
| RRID: | AB_2843093 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9899, RRID:AB_2843093.
Fold/Unfold
54 kDa VacA-interacting protein; 54 kDa vimentin-interacting protein; 90 kDa SH3 protein interacting with Nck; AF3p21; dia interacting protein; Dia-interacting protein 1; Diaphanous protein-interacting protein; DIP; DIP-1; DIP1; NCK interacting protein with SH3 domain; NCK-interacting protein with SH3 domain; Nckipsd; ORF1; SH3 adapter protein SPIN90; SH3 protein interacting with Nck 90 kDa; SPIN90; SPN90_HUMAN; VIP54; WASLBP; WASP-interacting SH3-domain protein; WISH; wiskott- Aldrich syndrome protein-interacting protein; Wiskott-Aldrich syndrome protein-interacting protein;
Immunogens
Highest expression in heart, brain, skeletal muscle, kidney and liver. Lower levels in placenta, lung, small intestine and leukocytes. Weak expression in colon, thymus and spleen.
- Q9NZQ3 SPN90_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MYRALYAFRSAEPNALAFAAGETFLVLERSSAHWWLAARARSGETGYVPPAYLRRLQGLEQDVLQAIDRAIEAVHNTAMRDGGKYSLEQRGVLQKLIHHRKETLSRRGPSASSVAVMTSSTSDHHLDAAAARQPNGVCRAGFERQHSLPSSEHLGADGGLYQIPLPSSQIPPQPRRAAPTTPPPPVKRRDREALMASGSGGHNTMPSGGNSVSSGSSVSSTSLDTLYTSSSPSEPGSSCSPTPPPVPRRGTHTTVSQVQPPPSKASAPEPPAEEEVATGTTSASDDLEALGTLSLGTTEEKAAAEAAVPRTIGAELMELVRRNTGLSHELCRVAIGIIVGHIQASVPASSPVMEQVLLSLVEGKDLSMALPSGQVCHDQQRLEVIFADLARRKDDAQQRSWALYEDEGVIRCYLEELLHILTDADPEVCKKMCKRNEFESVLALVAYYQMEHRASLRLLLLKCFGAMCSLDAAIISTLVSSVLPVELARDMQTDTQDHQKLCYSALILAMVFSMGEAVPYAHYEHLGTPFAQFLLNIVEDGLPLDTTEQLPDLCVNLLLALNLHLPAADQNVIMAALSKHANVKIFSEKLLLLLNRGDDPVRIFKHEPQPPHSVLKFLQDVFGSPATAAIFYHTDMMALIDITVRHIADLSPGDKLRMEYLSLMHAIVRTTPYLQHRHRLPDLQAILRRILNEEETSPQCQMDRMIVREMCKEFLVLGEAPS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NZQ3 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K84 | Ubiquitination | Uniprot | |
| Y85 | Phosphorylation | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| R107 | Methylation | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| S113 | Phosphorylation | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| S122 | Phosphorylation | Uniprot | |
| S147 | Phosphorylation | Uniprot | |
| Y161 | Phosphorylation | P12931 (SRC) | Uniprot |
| T180 | Phosphorylation | Uniprot | |
| T181 | Phosphorylation | Uniprot | |
| Y227 | Phosphorylation | P12931 (SRC) | Uniprot |
| R249 | Methylation | Uniprot | |
| T251 | Phosphorylation | Uniprot | |
| T253 | Phosphorylation | Uniprot | |
| T254 | Phosphorylation | Uniprot | |
| S256 | Phosphorylation | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| T311 | Phosphorylation | Uniprot | |
| Y404 | Phosphorylation | Uniprot | |
| K584 | Ubiquitination | Uniprot | |
| Y673 | Phosphorylation | Uniprot | |
| K712 | Ubiquitination | Uniprot |
Research Backgrounds
Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1). Induces microspike formation, in vivo (By similarity). In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42 (By similarity). May play an important role in the maintenance of sarcomeres and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion. Plays a role in angiogenesis.
Nucleus.
Note: Colocalizes with DRF1 at membrane ruffles, and with Nck at Z-disks in mature cardiac myocytes.
Highest expression in heart, brain, skeletal muscle, kidney and liver. Lower levels in placenta, lung, small intestine and leukocytes. Weak expression in colon, thymus and spleen.
Associates with the intermediate filaments, vimentin and desmin. Binds the first and third SH3 domains of NCK. Binds the proline-rich domains of N-WASP through its SH3 domain (By similarity). Similarly, binds diaphanous protein homolog 1 (DRF1). Binds the SH3 domains of GRB2 through its proline-rich domains. Interacts with Helicobacter pylori toxin vacA. Isoform 4 interacts with FHOD1. Interacts with FASLG. Interacts with TMIGD2.
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