SH2B3 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of extracts from EC304 cells, using SH2B3 Antibody.

DF9898 at 1/100 staining Rat brain tissue by IHC-P.

Product:	SH2B3 Antibody
Catalog:	DF9898
Description:	Rabbit polyclonal antibody to SH2B3
Application:	WB IHC IF/ICC
Reactivity:	Human
Mol.Wt.:	63 kDa, 80 kDa; 63kD(Calculated).
Uniprot:	Q9UQQ2
RRID:	AB_2843092

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

SH2B3 Antibody detects endogenous levels of total SH2B3.

RRID:

AB_2843092
Cite Format: Affinity Biosciences Cat# DF9898, RRID:AB_2843092.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

IDDM20; Lymphocyte adapter protein; Lymphocyte specific adapter protein; Lymphocyte specific adapter protein Lnk; Lymphocyte-specific adapter protein Lnk; SH2B adapter protein 3; SH2B adaptor protein 3; Sh2b3; SH2B3_HUMAN; Signal transduction protein Lnk;

Immunogens

Immunogen:

Uniprot:

Q9UQQ2(SH2B3_HUMAN) >>Visit HPA database.

Gene(ID):

SH2B3(10019)

Expression:

Q9UQQ2 SH2B3_HUMAN:

Preferentially expressed by lymphoid cell lines.

Sequence:

Q9UQQ2 SH2B3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNGPALQPSSPSSAPSASPAAAPRGWSEFCELHAVAAARELARQYWLFAREHPQHAPLRAELVSLQFTDLFQRYFCREVRDGRAPGRDYRDTGRGPPAKAEASPEPGPGPAAPGLPKARSSEELAPPRPPGPCSFQHFRRSLRHIFRRRSAGELPAAHTAAAPGTPGEAAETPARPGLAKKFLPWSLAREPPPEALKEAVLRYSLADEASMDSGARWQRGRLALRRAPGPDGPDRVLELFDPPKSSRPKLQAACSSIQEVRWCTRLEMPDNLYTFVLKVKDRTDIIFEVGDEQQLNSWMAELSECTGRGLESTEAEMHIPSALEPSTSSSPRGSTDSLNQGASPGGLLDPACQKTDHFLSCYPWFHGPISRVKAAQLVQLQGPDAHGVFLVRQSETRRGEYVLTFNFQGIAKHLRLSLTERGQCRVQHLHFPSVVDMLHHFQRSPIPLECGAACDVRLSSYVVVVSQPPGSCNTVLFPFSLPHWDSESLPHWGSELGLPHLSSSGCPRGLSPEGLPGRSSPPEQIFHLVPSPEELANSLQHLEHEPVNRARDSDYEMDSSSRSHLRAIDNQYTPL

PTMs - Q9UQQ2 As Substrate

Q9UQQ2

Site	PTM Type	Enzyme	Source
S10	Phosphorylation		Uniprot
S16	Phosphorylation		Uniprot
S18	Phosphorylation		Uniprot
K99	Ubiquitination		Uniprot
S103	Phosphorylation		Uniprot
S120	Phosphorylation		Uniprot
S150	Phosphorylation		Uniprot
T159	Phosphorylation		Uniprot
T165	Phosphorylation		Uniprot
Y203	Phosphorylation		Uniprot
Y273	Phosphorylation	P43403 (ZAP70) , P06239 (LCK)	Uniprot
S330	Phosphorylation		Uniprot
S337	Phosphorylation		Uniprot
S343	Phosphorylation		Uniprot
K373	Ubiquitination		Uniprot
S511	Phosphorylation		Uniprot
S553	Phosphorylation		Uniprot
Y555	Phosphorylation		Uniprot
S559	Phosphorylation		Uniprot
S560	Phosphorylation		Uniprot
Y572	Phosphorylation		Uniprot

Research Backgrounds

Q9UQQ2_SH2B3_HUMAN

Function:

Links T-cell receptor activation signal to phospholipase C-gamma-1, GRB2 and phosphatidylinositol 3-kinase.

PTMs:

Tyrosine phosphorylated by LCK.

Tissue Specificity:

Preferentially expressed by lymphoid cell lines.

Subunit Structure:

Binds to the tyrosine-phosphorylated TCR zeta chain via its SH2 domain.

Family&Domains:

Belongs to the SH2B adapter family.

Research Fields

· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)

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SH2B3 Antibody - #DF9898