SERPINB10 Antibody | Affinity Biosciences

Cites

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Figure 6 Western Blot Validation of Proteins in Neutrophil Activation Networks.

Product:	SERPINB10 Antibody
Catalog:	DF9894
Description:	Rabbit polyclonal antibody to SERPINB10
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Horse, Rabbit, Dog
Mol.Wt.:	45 kDa; 45kD(Calculated).
Uniprot:	P48595
RRID:	AB_2843088

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Horse(91%), Rabbit(91%), Dog(82%)

Clonality:

Polyclonal

Specificity:

SERPINB10 Antibody detects endogenous levels of total SERPINB10.

RRID:

AB_2843088
Cite Format: Affinity Biosciences Cat# DF9894, RRID:AB_2843088.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Bomapin; Peptidase inhibitor 10; PI-10; PI10; Protease inhibitor 10; Protease inhibitor 10 (ovalbumin type bomapin); Serpin B10; Serpin peptidase inhibitor clade B (ovalbumin) member 10; SERPINB10; SPB10_HUMAN;

Immunogens

Immunogen:

Uniprot:

P48595(SPB10_HUMAN) >>Visit HPA database.

Gene(ID):

SERPINB10(5273)

Expression:

P48595 SPB10_HUMAN:

Expressed specifically in myeloid cells and the bone marrow.

Sequence:

P48595 SPB10_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDSLATSINQFALELSKKLAESAQGKNIFFSSWSISTSLTIVYLGAKGTTAAQMAQVLQFNRDQGVKCDPESEKKRKMEFNLSNSEEIHSDFQTLISEILKPNDDYLLKTANAIYGEKTYAFHNKYLEDMKTYFGAEPQPVNFVEASDQIRKDINSWVERQTEGKIQNLLPDDSVDSTTRMILVNALYFKGIWEHQFLVQNTTEKPFRINETTSKPVQMMFMKKKLHIFHIEKPKAVGLQLYYKSRDLSLLILLPEDINGLEQLEKAITYEKLNEWTSADMMELYEVQLHLPKFKLEDSYDLKSTLSSMGMSDAFSQSKADFSGMSSARNLFLSNVFHKAFVEINEQGTEAAAGSGSEIDIRIRVPSIEFNANHPFLFFIRHNKTNTILFYGRLCSP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Rabbit

Dog

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P48595 As Substrate

P48595

Site	PTM Type	Enzyme	Source
S307	Phosphorylation		Uniprot
S318	Phosphorylation		Uniprot

Research Backgrounds

P48595_SPB10_HUMAN

Function:

Protease inhibitor that may play a role in the regulation of protease activities during hematopoiesis and apoptosis induced by TNF. May regulate protease activities in the cytoplasm and in the nucleus.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Mostly found in the nucleus.

Tissue Specificity:

Expressed specifically in myeloid cells and the bone marrow.

Family&Domains:

Belongs to the serpin family. Ov-serpin subfamily.

Research Fields

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

References

1). Proteomic analyses reveal higher levels of neutrophil activation in men than in women with systemic lupus erythematosus. Frontiers in Immunology, 2022 (PubMed: 35799787) [IF=7.3]

Application: WB Species: Human Sample:

Figure 6 Western Blot Validation of Proteins in Neutrophil Activation Networks. (A) Western blot analysis of BST2, PGAM1 and SERPINB10 in male SLE and female SLE. (B) Boxplot showing gray value of BST2 in male SLE and female SLE. (C) Boxplot showing gray value of PGAM1 in male SLE and female SLE. (D) Boxplot showing gray value of SERPINB10 in male SLE and female SLE.

2). Non-Esterified Fatty Acid-Induced Apoptosis in Bovine Granulosa Cells via ROS-Activated PI3K/AKT/FoxO1 Pathway. Antioxidants, 2023 (PubMed: 36829992) [IF=7.0]

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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SERPINB10 Antibody - #DF9894