RIOK1 Antibody | Affinity Biosciences

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Western blot analysis of RIOK1 using HeLa whole cell lysates.

Product:	RIOK1 Antibody
Catalog:	DF9888
Description:	Rabbit polyclonal antibody to RIOK1
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.:	66 kDa; 66kD(Calculated).
Uniprot:	Q9BRS2
RRID:	AB_2843082

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(92%), Bovine(100%), Horse(92%), Sheep(100%), Dog(92%), Xenopus(92%)

Clonality:

Polyclonal

Specificity:

RIOK1 Antibody detects endogenous levels of total RIOK1.

RRID:

AB_2843082
Cite Format: Affinity Biosciences Cat# DF9888, RRID:AB_2843082.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AD034; bA288G3.1; RIO kinase 1 (yeast); RIO kinase 1; RIOK1; RIOK1_HUMAN; Serine/threonine-protein kinase rio1;

Immunogens

Immunogen:

Uniprot:

Q9BRS2(RIOK1_HUMAN) >>Visit HPA database.

Gene(ID):

RIOK1(83732)

Sequence:

Q9BRS2 RIOK1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDYRRLLMSRVVPGQFDDADSSDSENRDLKTVKEKDDILFEDLQDNVNENGEGEIEDEEEEGYDDDDDDWDWDEGVGKLAKGYVWNGGSNPQANRQTSDSSSAKMSTPADKVLRKFENKINLDKLNVTDSVINKVTEKSRQKEADMYRIKDKADRATVEQVLDPRTRMILFKMLTRGIITEINGCISTGKEANVYHASTANGESRAIKIYKTSILVFKDRDKYVSGEFRFRHGYCKGNPRKMVKTWAEKEMRNLIRLNTAEIPCPEPIMLRSHVLVMSFIGKDDMPAPLLKNVQLSESKARELYLQVIQYMRRMYQDARLVHADLSEFNMLYHGGGVYIIDVSQSVEHDHPHALEFLRKDCANVNDFFMRHSVAVMTVRELFEFVTDPSITHENMDAYLSKAMEIASQRTKEERSSQDHVDEEVFKRAYIPRTLNEVKNYERDMDIIMKLKEEDMAMNAQQDNILYQTVTGLKKDLSGVQKVPALLENQVEERTCSDSEDIGSSECSDTDSEEQGDHARPKKHTTDPDIDKKERKKMVKEAQREKRKNKIPKHVKKRKEKTAKTKKGK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Horse

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BRS2 As Substrate

Q9BRS2

Site	PTM Type	Source
S21	Phosphorylation	Uniprot
S22	Phosphorylation	Uniprot
S24	Phosphorylation	Uniprot
K81	Ubiquitination	Uniprot
Y83	Phosphorylation	Uniprot
S89	Phosphorylation	Uniprot
R95	Methylation	Uniprot
S102	Phosphorylation	Uniprot
T107	Phosphorylation	Uniprot
K124	Ubiquitination	Uniprot
T175	Phosphorylation	Uniprot
S204	Phosphorylation	Uniprot
K218	Ubiquitination	Uniprot
Y223	Phosphorylation	Uniprot
S225	Phosphorylation	Uniprot
K299	Ubiquitination	Uniprot
T410	Phosphorylation	Uniprot
K411	Methylation	Uniprot
K426	Methylation	Uniprot
K426	Ubiquitination	Uniprot
K438	Ubiquitination	Uniprot
Y440	Phosphorylation	Uniprot
K451	Ubiquitination	Uniprot
Y466	Phosphorylation	Uniprot
T468	Phosphorylation	Uniprot
T470	Phosphorylation	Uniprot
K473	Ubiquitination	Uniprot
K474	Ubiquitination	Uniprot
K481	Ubiquitination	Uniprot
T494	Phosphorylation	Uniprot
T509	Phosphorylation	Uniprot
K539	Ubiquitination	Uniprot

Research Backgrounds

Q9BRS2_RIOK1_HUMAN

Function:

Involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in processing of 18S-E pre-rRNA to the mature 18S rRNA. Required for the recycling of NOB1 and PNO1 from the late 40S precursor. The association with the very late 40S subunit intermediate may involve a translation-like checkpoint point cycle preceeding the binding to the 60S ribosomal subunit (By similarity). Despite the protein kinase domain is proposed to act predominantly as an ATPase (By similarity). The catalytic activity regulates its dynamic association with the 40S subunit (By similarity). In addition to its role in ribosomal biogenesis acts as an adapter protein by recruiting NCL/nucleolin the to PRMT5 complex for its symmetrical methylation.

Subcellular Location:

Cytoplasm>Cytosol.

Subunit Structure:

Associates with the precursor of the 40S ribosome subunit. Interacts (via its N-terminus) with PRMT5 (via its N-terminus). Interacts with WDR77. Found in a PRMT5 complex composed of PRMT5, WDR77 and RIOK1. Interacts (via its C-terminus) with NCL; this interaction targets NCL for PRTM5 methylation.

Family&Domains:

Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.

Research Fields

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

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RIOK1 Antibody - #DF9888