SEPT5 Antibody - #DF9881

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Product: SEPT5 Antibody
Catalog: DF9881
Description: Rabbit polyclonal antibody to SEPT5
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Dog, Chicken, Xenopus
Mol.Wt.: 43 kDa; 43kD(Calculated).
Uniprot: Q99719
RRID: AB_2843075

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Dog(100%), Chicken(91%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
SEPT5 Antibody detects endogenous levels of total SEPT5.
RRID:
AB_2843075
Cite Format: Affinity Biosciences Cat# DF9881, RRID:AB_2843075.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5 Sep; CDCrel 1; CDCREL; CDCrel-1; CDCREL1; Cell division control related protein 1; Cell division control-related protein 1; H5; HCDCREL 1; OTTHUMP00000197271; OTTHUMP00000197272; OTTHUMP00000197273; OTTHUMP00000197274; OTTHUMP00000197275; OTTHUMP00000197276; OTTHUMP00000197396; OTTHUMP00000197410; Peanut like 1; Peanut like 1 homolog; Peanut like protein 1; Peanut-like protein 1; Platelet glycoprotein Ib beta chain; PNUTL1; SEPT5; SEPT5_HUMAN; Septin 5; Septin-5;

Immunogens

Immunogen:
Uniprot:

Q99719(SEPT5_HUMAN) >>Visit HPA database.

Gene(ID):
SEPT5(5413)
Expression:
Q99719 SEPT5_HUMAN:

Expressed at high levels in the CNS, as well as in heart and platelets (at protein level).

Sequence:
MSTGLRYKSKLATPEDKQDIDKQYVGFATLPNQVHRKSVKKGFDFTLMVAGESGLGKSTLVHSLFLTDLYKDRKLLSAEERISQTVEILKHTVDIEEKGVKLKLTIVDTPGFGDAVNNTECWKPITDYVDQQFEQYFRDESGLNRKNIQDNRVHCCLYFISPFGHGLRPVDVGFMKALHEKVNIVPLIAKADCLVPSEIRKLKERIREEIDKFGIHVYQFPECDSDEDEDFKQQDRELKESAPFAVIGSNTVVEAKGQRVRGRLYPWGIVEVENQAHCDFVKLRNMLIRTHMHDLKDVTCDVHYENYRAHCIQQMTSKLTQDSRMESPIPILPLPTPDAETEKLIRMKDEELRRMQEMLQRMKQQMQDQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Xenopus
91
Chicken
91
Horse
0
Sheep
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99719 As Substrate

Site PTM Type Enzyme
R6 Methylation
T13 Phosphorylation
Y24 Phosphorylation
T29 Phosphorylation
K41 Ubiquitination
S77 Phosphorylation
S225 Phosphorylation
S249 Phosphorylation
T251 Phosphorylation
K318 Ubiquitination
S327 Phosphorylation
T336 Phosphorylation
T341 Phosphorylation
K343 Ubiquitination
K363 Methylation

Research Backgrounds

Function:

Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in platelet secretion (By similarity).

PTMs:

In platelets, phosphorylated in response to thrombin, phorbol-12-myristate-13-acetate and collagen.

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton.
Note: In platelets, found in areas surrounding alpha-granules.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed at high levels in the CNS, as well as in heart and platelets (at protein level).

Subunit Structure:

Septins polymerize into heterooligomeric protein complexes that form filaments, and can associate with cellular membranes, actin filaments and microtubules. GTPase activity is required for filament formation (By similarity). Interacts with SEPTIN2 and SEPTIN5. In platelets, associated with a complex containing STX4. Interacts with PRKN. This interaction leads to SEPTIN5 ubiquitination and degradation (By similarity).

Family&Domains:

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

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