ROBO1 Antibody - #DF9877
| Product: | ROBO1 Antibody |
| Catalog: | DF9877 |
| Description: | Rabbit polyclonal antibody to ROBO1 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| Mol.Wt.: | 181 kDa; 181kD(Calculated). |
| Uniprot: | Q9Y6N7 |
| RRID: | AB_2843071 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9877, RRID:AB_2843071.
Fold/Unfold
Deleted in U twenty twenty; DUTT 1; DUTT1; FLJ21882; H Robo 1; H-Robo-1; hRobo 1; Robo 1; Robo1; ROBO1_HUMAN; Roundabout 1; Roundabout axon guidance receptor homolog 1; Roundabout homolog 1; Roundabout homolog1 precurser; Roundabout1; SAX 3; SAX3;
Immunogens
- Q9Y6N7 ROBO1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKWKHVPFLVMISLLSLSPNHLFLAQLIPDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPAAQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIVEQNKLNKDYRANDTVPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKLKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSMDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y6N7 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T42 | Phosphorylation | Uniprot | |
| S75 | Phosphorylation | Uniprot | |
| S80 | Phosphorylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| S251 | Phosphorylation | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| T321 | Phosphorylation | Uniprot | |
| T520 | Phosphorylation | Uniprot | |
| T660 | O-Glycosylation | Uniprot | |
| S661 | O-Glycosylation | Uniprot | |
| K743 | Ubiquitination | Uniprot | |
| K750 | Ubiquitination | Uniprot | |
| S855 | Phosphorylation | Uniprot | |
| S930 | Phosphorylation | Uniprot | |
| Y932 | Phosphorylation | Uniprot | |
| K937 | Ubiquitination | Uniprot | |
| S940 | Phosphorylation | Uniprot | |
| T942 | Phosphorylation | Uniprot | |
| T944 | Phosphorylation | Uniprot | |
| T946 | Phosphorylation | Uniprot | |
| T948 | Phosphorylation | Uniprot | |
| Y949 | Phosphorylation | Uniprot | |
| Y1019 | Phosphorylation | Uniprot | |
| K1026 | Ubiquitination | Uniprot | |
| Y1038 | Phosphorylation | P00519 (ABL1) | Uniprot |
| K1046 | Ubiquitination | Uniprot | |
| K1051 | Ubiquitination | Uniprot | |
| S1055 | Phosphorylation | Uniprot | |
| K1059 | Ubiquitination | Uniprot | |
| Y1073 | Phosphorylation | P00519 (ABL1) | Uniprot |
| T1075 | Phosphorylation | Uniprot | |
| S1094 | Phosphorylation | Uniprot | |
| K1100 | Ubiquitination | Uniprot | |
| K1106 | Ubiquitination | Uniprot | |
| Y1114 | Phosphorylation | P00519 (ABL1) | Uniprot |
| K1121 | Ubiquitination | Uniprot | |
| Y1126 | Phosphorylation | Uniprot | |
| R1237 | Methylation | Uniprot | |
| T1240 | Phosphorylation | Uniprot | |
| S1259 | Phosphorylation | Uniprot | |
| T1260 | Phosphorylation | Uniprot | |
| T1262 | Phosphorylation | Uniprot | |
| T1264 | Phosphorylation | Uniprot | |
| S1266 | Phosphorylation | Uniprot | |
| S1297 | Phosphorylation | Uniprot | |
| S1306 | Phosphorylation | Uniprot | |
| Y1311 | Phosphorylation | Uniprot | |
| Y1413 | Phosphorylation | Uniprot | |
| T1441 | Phosphorylation | Uniprot | |
| S1442 | Phosphorylation | Uniprot | |
| K1465 | Ubiquitination | Uniprot | |
| Y1476 | Phosphorylation | Uniprot | |
| S1492 | Phosphorylation | Uniprot | |
| T1494 | Phosphorylation | Uniprot | |
| S1497 | Phosphorylation | Uniprot | |
| S1528 | Phosphorylation | Uniprot | |
| Y1587 | Phosphorylation | Uniprot | |
| S1603 | Phosphorylation | Uniprot | |
| T1649 | Phosphorylation | Uniprot |
Research Backgrounds
Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA. May be required for lung development (By similarity).
Ubiquitinated. May be deubiquitinated by USP33.
Cell membrane>Single-pass type I membrane protein. Cell projection>Axon. Endoplasmic reticulum-Golgi intermediate compartment membrane>Single-pass membrane protein.
Note: Detected at growth cones in thalamus neurons. Detected at growth cones in thalamus neurons (By similarity). PRRG4 prevents cell surface location and both colocalize in the Endoplasmic reticulum/Golgi adjacent to the cell nucleus (By similarity).
Widely expressed, with exception of kidney.
Homodimer. Dimerization is mediated by the extracellular domain and is independent of SLIT liganding. Interacts with SLIT1 (By similarity). Interacts with SLIT2. Interacts with FLRT3. Interacts with MYO9B (via Rho-GAP domain).
Belongs to the immunoglobulin superfamily. ROBO family.
Research Fields
· Organismal Systems > Development > Axon guidance. (View pathway)
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