RRAS2 Antibody - #DF9840

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Product: RRAS2 Antibody
Catalog: DF9840
Description: Rabbit polyclonal antibody to RRAS2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 23 kDa; 23kD(Calculated).
Uniprot: P62070
RRID: AB_2843034

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
RRAS2 Antibody detects endogenous levels of total RRAS2.
RRID:
AB_2843034
Cite Format: Affinity Biosciences Cat# DF9840, RRID:AB_2843034.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C86394; Oncogene RRAS2; Oncogene TC21; Ras like protein TC21; Ras related protein R Ras2; Ras-like protein TC21; Ras-related protein R-Ras2; Related RAS viral (r ras) oncogene homolog 2; Related RAS viral oncogene homolog 2; RRAS 2; RRAS2; RRAS2_HUMAN; TC 21; TC21; Teratocarcinoma oncogene; Teratocarcinoma oncogene TC21;

Immunogens

Immunogen:
Uniprot:

P62070(RRAS2_HUMAN) >>Visit HPA database.

Gene(ID):
RRAS2(22800)
Expression:
P62070 RRAS2_HUMAN:

Ubiquitously present in all tissues examined, with the highest levels in heart, placenta, and skeletal muscle. Moderate levels in lung and liver; low levels in brain, kidney, and pancreas.

Sequence:
MAAAGWRDGSGQEKYRLVVVGGGGVGKSALTIQFIQSYFVTDYDPTIEDSYTKQCVIDDRAARLDILDTAGQEEFGAMREQYMRTGEGFLLVFSVTDRGSFEEIYKFQRQILRVKDRDEFPMILIGNKADLDHQRQVTQEEGQQLARQLKVTYMEASAKIRMNVDQAFHELVRVIRKFQEQECPPSPEPTRKEKDKKGCHCVIF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P62070 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S10 Phosphorylation
Y15 Phosphorylation
Y43 Phosphorylation
C55 S-Nitrosylation
Y105 Phosphorylation
K128 Ubiquitination
K177 Ubiquitination
C183 S-Nitrosylation
S186 Phosphorylation
T190 Phosphorylation
K192 Ubiquitination

Research Backgrounds

Function:

It is a plasma membrane-associated GTP-binding protein with GTPase activity. Might transduce growth inhibitory signals across the cell membrane, exerting its effect through an effector shared with the Ras proteins but in an antagonistic fashion.

PTMs:

May be post-translationally modified by both palmitoylation and polyisoprenylation.

Subcellular Location:

Cell membrane>Lipid-anchor>Cytoplasmic side.
Note: Inner surface of plasma membrane possibly with attachment requiring acylation of the C-terminal cysteine (By similarity with RAS).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously present in all tissues examined, with the highest levels in heart, placenta, and skeletal muscle. Moderate levels in lung and liver; low levels in brain, kidney, and pancreas.

Family&Domains:

Belongs to the small GTPase superfamily. Ras family.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

References

1). Whole-Transcriptome Analysis of Non-Coding RNA Alteration in Porcine Alveolar Macrophage Exposed to Aflatoxin B1. Toxins, 2022 (PubMed: 35737034) [IF=4.2]

Application: IF/ICC    Species: pig    Sample: 3D4/2 cells

Figure 7 Validation of miRNA-seq data availability with RT-qPCR and examination of the expression of NFKB1 and RRAS2 proteins with cell immunofluorescence assay. (A) Expression of miR-181a, miR-331-3p and miR-342 in 3D4/2 cells after 24 h AFB1 exposure. * Indicates extremely significant differences (p < 0.05), ** Indicates extremely significant differences (p < 0.01). All experiments were repeated 3 times. (B) The fluorescence intensity and positive percentages of NFKB1. Nuclear staining (blue) and NFKB1 positive cells (green). (C) The fluorescence intensity and positive percentages of RRAS2. Nuclear staining (blue) and NFKB1 positive cells (red). ** Indicates extremely significant differences (p < 0.01). All experiments were repeated 3 times.

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